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Episome is an additional genetic element that can exist either as an autonomous entity or be inserted into the continuity of the chromosome of a host cell.

附加体是能插入寄生细胞连续的染色体之中，也能以自主实体而存在的一种附加遗传因素。

To further elucidate the mechanism of BMP in regulating cell differentiation and to provide basis for BMP gene therapy, this study centers on the effects of BMP2 gene transfection on fibroblasts and contains the following works:① A 1.2kb fragment containing full length human BMP2 coding region was inserted into pBK-CMV (expressed both in protocaryon and eukaryon) by using predetermined orientation methods, resulting in phagemid expression vector for BMP2 (pBK-B2).② pBK-B2 was transfected into NIH3T3 cells by using LipofectAMINE.

为了进一步从分子水平探讨BMP对细胞分化的调控作用，并为BMP基因疗法的建立提供依据，本实验围绕BMP2基因转染对成纤维细胞的生物学作用进行了以下几个方面的工作：①将含有人BMP2全长编码序列的基因片段(1.2kb)定向克隆于噬菌粒表达载体pBK-CMV中，成功构建了人BMP2噬菌粒表达载体pBK-B2。

In sham operation group,the external carotid artery was surgically prepared for insertion of the filament,but the filament was not inserted.

健康雄性SD大鼠，体重250～300 g，随机分为假手术组、缺血组、L NA治疗组。

Ti underlayer improved the magnetic and high recording characteristics of the single CoCr film and CoCr/FeNi double layer media, CoCr/FeNi double layer floppy disk with inserted Ti underlayer have more smooth surface and better envelope curve.

钛膜底层的插入使得上部磁层的结晶取向及磁特性明显改善，结晶和磁性的均匀性提高。用钛膜打底的软盘，随t〓减薄，记录灵敏度提高，重放电压增加。

But the Ti layer inserted between CoCr film and FeNi film, decreases the reproduced volatage of low density and D〓, when the main pole is 0.2μm thick and the track density is 256 TPI, the bits density D〓 is larger than 100KBPI for the manufactured floppy disks.

但钛膜插在CoCr和FeNi之间时，尽管只有10nm，却使重放输出和记录密度都下降。所研制的软盘取磁头主磁极厚度T〓=0.2μm，道密度为256TPI情况下，位密度D〓＞100KBPI。

The minor core protein s C-encoding gene of Muscovy duck reovirus was cloned into theprokaryotic expression vector pET32a. The recombinant plasmid pET32a-s C was amplified andextracted after being transformed into E.coli DH5a competent cells. Restriction analysis withEcoRⅠand SacⅠand sequences analysis indicated that the recombinant plasmid was inserted withcorrect open reading frame. The fusion protein about 50 ku was produced after induction with 0.15mmol/L IPTG of E.coli competent cells transformed with pET32a-sC. The SDS-PAGE andWestern-bloting test indicated that the fusion protein reacted with the convalescents sera of duckinfected with Muscovy duck reovirus. The indirect ELISA method was developed by using thepurified fusion s C protein as coating antigen. The optimal concentration of s C was 5μg/ml, thedilution of serum sample was 1:40; The results showed that preparation of an ELISA by using sCas coating antigen in detecting 50 field duck sera in comparison with the AGIP were more sensitiveand specific than agar gel immuno-diffusion AGIP test. The results suggest that presence ofantibody against viral protein sC in duck may be a good indicator by the sC-ELISA for detectionof duck infection with reovirus.

同时，本研究将编码外壳蛋白σC的基因克隆于原核表达载体pET32a上，经过EcoRⅠ和SacⅠ双酶切鉴定和序列分析后，得到阳性重组质粒pET32a-σC；将阳性重组质粒pET32a-σC转化到大肠杆菌BL-21感受态细胞中进行诱导表达，经SDS-PAGE和Western-blbtting检测分析，融合表达的蛋白能够与番鸭呼肠孤病毒感染的康复鸭血清发生特异性反应；将融合表达的蛋白纯化后作为包被抗原，建立了检测鸭血清中呼肠孤病毒抗体的间接酶联免疫吸附试验检测方法，此方法中抗原的最佳包被浓度为5μg／ml、标准阳性血清的最适稀释倍数为1：40倍，用此方法对50份鸭血清样品进行检测，并与琼脂糖凝胶扩散试验检测抗体的法相比较，证明此ELISA方法具有良好的特异性和敏感性，本研究为今后鸭呼肠孤病毒诊断试剂盒的研制奠定了基础。

Methods Twenty-seven patients suffering from mallet finger accompanied by avulsional fracture were treated with Ishiguro method or its modified method. During the operation, a blocking pin was inserted percutaneously with DIP flexed, then the DIP was fixed at a position of full extension with another Kirschner wire. When the fracture fragment was big with rotation displacement, a third Kirschner wire could be used to correct the displacement and fix fracture. Results The patients were followed up 2 months to 6 years and 6 months with an average of 9.3 months postoperatively.

采用石黑法及其改良法治疗27例伴撕脱骨折的锤状指，在透视下运用闭合穿针的方法，在远侧指间关节屈曲位时于背侧插入阻挡针，然后用另一根克氏针将DIP固定于充分伸直位；当较大的骨折块向背侧有旋转移位时，可加用一根克氏针撬拨复位并固定骨折块。

Will be placed on the net pot fire, inserted broth.

将净锅置于火上，添入高汤。

As the simulated computation shows, under the simple condition of initial firing data, only two trajectories are used to approach the target with one more inserted value.

经模拟计算表明：在初始射角赋予非常简单的条件下，只用两条弹道，再通过一次插值，即可逼近目标。

This article respectively introduced finally the method of flash inserting the PowerPoint and the web page at last. This courseware is used in class, so it is inserted into the PowerPoint.

最后还分别介绍了flash插入PowerPoint和网页的方法，鉴于此课件是上课教学之用，故将其插入PowerPoint中。

Shop assistant: Please check at the door.

店员：请您到门口柜台交费。

In the end, transportation of PAHs in the environmental matrix was preliminarily investigated and discussed according to PAHs data of soils and sediments from a certain region in Shanghai. The result indicated that there are two or more pathways on PAHs transportation in the environmental matrix.

最后，对上海特定地区内PAHs在环境介质中的迁移径途进行了分析讨论，结果表明上海市土壤和沉积物等环境介质中PAHs的迁移存在多种途径。