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Methods The total RNA was acquired from young rabbit particular cartilage, the BMP7 gene was inserted into pcDNA-3.1 to construct eukaryotic expression vector plasmid of pcDNA3.1-BMP7; the target gene-BMP7 was identified respectively by PCR analysis, restriction enounces analysis and nucleotide sequencing; the plasmid was transfected into adult rabbit knee articular chondrocytes, then the expression of BMP7 was detected by in situ hybridization, PCR and Western blotting.

从体外培养的幼兔膝关节软骨细胞中提取总RNA；按GenBank BMP7基因序列化学合成2条引物，采用RT-PCR方法得到BMP7基因；将BMP7基因片段插入到真核表达载体pcDNA 3.1中，构建pcDNA3.1-BMP7真核表达载体质粒；利用双酶切、PCR及核苷酸序列分析鉴定BMP7目的基因；将重组pcDNA3.1-BMP7真核表达载体质粒转染家兔关节软骨细胞，分别采用原位杂交、PCR、Western blotting法测定BMP-7表达。

Obtains by this idea, regards as the platform technology the modern system design method epitomizing, has discussed its composition important document and the realization method, and introduced it has inserted the type system the domain.

以此观念入手，将平台技术视为现代系统设计方法的集大成者，探讨了其组成要件和实现方法，并将其引入了嵌入式系统的领域。

Methods The bile duct of rabbit was excided and a pipe supporting bile duct was inserted through duodenal major papilla to establish a model. V.G staining and TGF-beta 1 immunohistochemistry staining on tubal wall of rabbit bile duct in different times were used to determine quantitatively the intensity of light TGF-beta 1 positive area and the relationship between the integral-optical density of bile duct tissue collagen content and time.

切开兔胆管并自十二指肠乳头行胆管内支撑方法建立模型，将不同时期梗阻段胆管壁进行V.G染色和β1型转化生长因子（transforming growth factor beta 1，TGF-β1）免疫组化染色，测定TGF-β1阳性区域的光度以及胆管组织胶原含量积分光密度随时间变化关系。

Shortly after Garuda's midnight, the eight-member team was inserted by Garudan flitters to within twenty miles of the crater, allowing them ample time to reach the farm-hive before sunrise.

嘎鲁达的午夜刚过，这个8人小队就由嘎鲁达搬运机输送到距离环形山20英里以内的地方，这样他们就有充足的时间在日出之前到达农场母巢。

She was in hospital, being force-fed lentil soup through a tube inserted into her nose.

因为她正在医院住院，插着鼻管，强迫接受扁豆汤饲喂。

In future, will there be surgically inserted ID chips on our forearms like in the movies?

&在未来，我们的前臂上面是否就真的像电影里边看到的一样，被植入ID芯片？&

It helps not to be doubled up with hysterical laughter when you have a length of hosing inserted into your backside.

当有一段软管状物体插入阁下后庭的时候，避免歇斯底里般地笑弯腰相信这一良言对你是有好处的。

According to their position in words, English affixes are divided into prefixes and suffixes (some languages have infixes , bound morphemes which are inserted into other morphemes).

根据他们的立场的话，英语词缀分为前缀和后缀（有些语言有infixes ，约束语素是插入其他语素）。

Likewise we confirm, approve and renew with the approval of the sacred council, the letters themselves along with their decrees, declarations, prohibitions, commands, exhortations, warnings, applications of ecclesiastical interdicts, and other sentences, censures and penalties, whether by canonical sanctions or by our own act, especially those in the letter summoning this sacred universal council, and each and all of the other clauses contained in the said letters, the meanings of which we wish to be considered as expressed as if they were inserted herein word for word, even though, as being definite and valid, they require no other confirmation or approval for a more extensive guarantee and demonstration of the truth.

同样，我们确认，批准和重新批准的神圣理事会，字母本身以及他们的法令，声明，禁止，命令，劝告，警告，应用教会interdicts ，和其他的判决，谴责和惩罚，无论是典型的制裁或由我们自己的行为，特别是那些在信中传唤这一神圣普遍理事会，每个和所有其他条款中所载的信件说，其中的意义，我们希望被看作是表示如果他们一词此处插入一句话，即使是明确的和有效的，他们不需要任何其他的确认或批准进行更广泛的保障和示范真相。

A stainless steel spring is inserted in the tube to reduce the risk of kinking or collapsing.

管体内置不锈钢弹簧，防管体压扁。

Shop assistant: Please check at the door.

店员：请您到门口柜台交费。

In the end, transportation of PAHs in the environmental matrix was preliminarily investigated and discussed according to PAHs data of soils and sediments from a certain region in Shanghai. The result indicated that there are two or more pathways on PAHs transportation in the environmental matrix.

最后，对上海特定地区内PAHs在环境介质中的迁移径途进行了分析讨论，结果表明上海市土壤和沉积物等环境介质中PAHs的迁移存在多种途径。