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Expression of Bilirubin Oxidase cDNA from Myrothecium Verrucaria in E. coliThe 1. 6kb bilirubin oxidase cDNA from Myrothecium Verrucaria was excised from plasmid pGEM-T/BOX, and inserted into expression vector, pET-3a which were under the control of a T7 promoter.

用限制性内切酶将1.6kb疣疱漆斑菌胆红素氧化酶cDNA从质粒pGEM-T／BOX切下，插入到表达质粒pET-3a的T7启动子下游，构建成疣疱漆斑菌胆红素氧化酶表达质粒pET-3a／BOX。

Control wiring plug 10.2 can only be inserted or emoved when the the withdrawable part is in he test/disconnected position.

只有接地开关处在分闸位置时，手车方可进入工作位置

The minimal ventricular rates ranged from 46 to 80 beats per minute. VVJ mode pacemakers were inserted through xyphoid approach in all live-birth infants. Complications were noted in three of them (50%). Antinuclear antibody and anti-SSA/Ro antibody were positive in all 8 mothers (100%). The anti-SSB/La antibody was positive in 6 of the eight mothers (75%). Five infants tested positive for anti-SSA/Ro antibody.

所有活产婴孩均经由剑突下途迳植入心律调节器，3个(50%)有并发症。8个母亲(100%)有阳性抗核抗体及anti-SSA/Ro抗体，6个母亲有阳性anti-SSB/La抗体。5个婴孩有阳性anti-SSA/Ro抗体，所有婴孩均没有anti-SSB/La抗体。

A highly conserved sequence of 210 bp of AtNHX1 gene that encodes a Na+/H+ antiport protein was isolated from A.thaliana,the 210 bp fragment was amplified as RNAi target site and used in construction of RNAi vector,and was inserted into pHANNIBAL as reverted repeat with an intron.The expressional box was linked into the vector of pART27,NPTⅡgene is the screening marker in this vector.

利用RNAi研究植物基因功能，可选择靶基因的一段单一序列使基因家族中的单个成员沉默；也可以选择某一基因家族的多个成员具有的一段同源性很高的保守序列，设计针对这一序列的dsRNA表达载体使多个基因同时沉默，RNAi成为功能基因组学研究的重要手段。

Methods The left anterior descending coronary artery was ligated and gas embolism was performed through vein tube inserted distal to the ligated site of LAD in 6 dogs,while 8 dogs underwent ligation of LAD.cTnI,CK,CK-MB,LDH and electrocardiographic parameters were evaluated before,and 30min,60min,90min,120min,and 180min after occlusion.

最后用红四氮唑和伊凡氏蓝染色确定心肌梗死区和缺血危险区并称其湿重，计算二者比值。结果结扎前降支血管后30、60、90、120、180min时间点，实验组cTnI、CK、NST和∑ST较对照组明显高，差异有统计学意义(P.05)，在部分时间点LDH、CK-MB亦升高(P.05)。实验组心肌梗死与缺血危险区比值(0.58±0.16)%较对照组(0.29±0.31)%明显高，差异有统计学意义(P.05)。对照组2例(25%)发生致死性室颤。

The activity of neurones in RVLM was recorded extracellularly with single barrelled glass microelectrodes. Monopolar stimulating electrodes were inserted into the defense areas of hypothalamus and midbrain (dorsal periaqueductal grey, dPAG).

头端延髓腹外侧区是心血管交感活动的基本中枢，其神经元不仅参与基础心血管活动的维持，而且能对不同状态下的信息进行整合，从而调节心血管活动水平以适应不同状态下机体需要。

The text is automatically inserted into a text box in the same Journal note.

文字会自动插入到当前日记本便笺的文字框。

Some thin wire is inserted under canvas to help shape the final positioning.

在伞布的底下要插入些细线，以保证最后的定型。

They will be inserted in to the groove before assembly.

它们必须在装配前被放进型材槽中。

They will be inserted in to the groove before assembly.

它们必须在装配前被塞进型材槽中。

Shop assistant: Please check at the door.

店员：请您到门口柜台交费。

In the end, transportation of PAHs in the environmental matrix was preliminarily investigated and discussed according to PAHs data of soils and sediments from a certain region in Shanghai. The result indicated that there are two or more pathways on PAHs transportation in the environmental matrix.

最后，对上海特定地区内PAHs在环境介质中的迁移径途进行了分析讨论，结果表明上海市土壤和沉积物等环境介质中PAHs的迁移存在多种途径。