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Methods:The conservative region of mTOR gene was inserted into pLXIN reversedly, then the vector was packaged in PT67 cells by transfection with lipofectamine and transfected to VSMC. The efficiency of antisense inhibition was verified,and the changes of p70S6k and 4E-BP1 were also determined at the same time. The proliferation activity was determined by flowcytometry and MTT.

采用脂质体介导转染包装细胞后构建mTOR的反义重组逆转录病毒载体(pLXIN-AmTOR)感染VSMC，检测mTOR及其下游底物包括真核细胞启动子4E结合蛋白1(4E-BP1)和核糖体蛋白S6激酶(p70S6k)的表达变化，以及VSMC细胞增殖周期和增殖活性的改变。

Stamens 5, inserted at margin of disc; filaments filiform-subulate; anthers ovoid to oblong. Disc ringlike.

雄蕊5，在花盘在边缘的着生的；钻形花丝丝状；花药卵球形的到长圆形。

The plasmid pAO815 is then used in homologous recombination, and the recombinant plasmid pETnsGH is further converted, jointed and inserted into GS115 saccharomycetes for the foreign gene to express normally.

再选择质粒pAO815进行同源重组，进而有效地将重组质粒pETnsGH转化接合插入GS115菌种中，使外源基因sGH正常表达。

Staminodes apically entire and sagittate, or 3-sect; fertile stamens 3 (sometimes 1 or 2 aborted), all inserted opposite sepals

顶部的退化雄蕊是整个和箭头形，或者3全裂；可育雄蕊3(有时1或2 中止)，全部着生的与萼片对生 8 Murdannia 水竹叶属

Stamens inserted in widened part of corolla tube; filaments short or almost none; anthers oblong or narrowly triangular, free from pistil head, base sagittate or deeply cordate and not spurred; disc absent.

深裂花冠筒的加宽的雄蕊着生于；花丝短或几乎没有一个；花药长圆形或狭三角形，离生的雌蕊头状花序，基部箭头形的或深心形而并非距；花盘无。

Stamens inserted at middle of corolla tube; filaments short; anthers sagittate, included, adherent to pistil head at middle, cells with an empty tail; disc ringlike, as long as ovary, apex entire or 5-lobed.

雄蕊花冠筒的着生的在中部；花丝短箭头形的花药，包括，对雌蕊头状花序在中部，室具附着一空有尾；花盘环形，倍于子房，先端全缘或者5浅裂。

Filaments free or ± connate, inserted inside at top of disk; connectives dilated abaxially from base or from apex into thin, scarious appendages exceeding anthers.

花丝 分离或者合生，在花盘顶部的里面着生；药隔背部从基部膨大或从先端缩小为细瘦的干膜质附属物，长于花药。

Perianth rotate, spreading, or erect, inserted at rim of receptacle tube; segments numerous, outer ones sepaloid, inner ones petaloid.

花被辐状，平展的，或者直立，着生在花托筒的凸缘；裂片多数，外部的萼片状，内部的瓣状。

In the part of hardware module, card inserted detection and reset, data reception and transmission, card deactivation are discussed separatedly according to the module's function; In the part of software module, smart card interface register configuration, transmission protocol parse and the applications of MIPS interrupt are discussed.

在硬件模块部分，根据接口模块实现的功能，分为卡插入检测及复位、数据收发、使卡无效并移出这几部分来分别讨论；在软件部分，讨论了对智能卡接口模块寄存器的配置、传输协议的解析以及MIPS处理器中断的应用。

At wall-floor connections a sheet of expanded polystyrene is inserted which acts as thermal insulation at this point and also as permanent shuttering.

在相邻面墙和地板的接缝中放进一层聚苯乙烯泡沫，使接缝处能充分的隔热防寒。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。