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In PM48, T-DNA was inserted into the ORF of the gene encoding a cell cycle-associated protein.

PM48的插入位点在一个编码细胞周期相关蛋白的基因ORF区。

After being digested with EcoR I and Xho I, they were inserted to the vectors pGEX-4T-l orientally, and then transformed into E.coli BL21(DE3) cells. The monoclone were selected and identified by means of restriction analysis, PCR and sequencing. As the result, the two recombinant plasmids with the different DNA fragements of the PrP gene were constructed and designated as pMY01[Ov rPrP(23-244)] and pMY02[Ov rPrP(91 -244)], respectively.

挑选单个菌落，提取重组质粒，经酶切、PCR扩增后琼脂糖凝胶电泳分析和测序鉴定，成功地构建了2种绵羊重组朊蛋白的表达质粒pMY01[OvrPrP(23～244)]和pMYO2[Ov rPrP(91～244)]。

In this study, the structure sequences of Shiga toxin 2B(stx2B) and Shiga toxin 1B(stx1B) were respectively amplified from EHEC O157:H7 by PCR. stx2B-L-stx1B fusion gene was constructed by a 12 bp linker ligating these two fragments. After digested with restriction endonuclease Nco Ⅰ and EcoR Ⅰ, the fusion gene was orientally inserted into expression vector pET28a.

利用PCR方法，从肠出血性大肠杆菌O157：H7的基因组DNA中，分别扩增出Stx1B和Stx2B亚基的结构基因片段，然后再通过PCR将这2个片段连接起来，并定向克隆到表达质粒pET28a的NcoⅠ和EcoRⅠ位点之间，构建了重组表达质粒pMB1。

Bisexual flowers: stamens 1 or 3, inserted on 1 side of ovary; filaments inconspicuous; connectives conspicuous, if stamens 3, connectives connate at base or free; anthers 1- or 2-loculed, longitudinally dehiscent; pistil 1, 1-carpelled; ovary inferior, 1-loculed; ovule 1, pendulous, orthotropous; style short or absent.

两性花：雄蕊1或者3，在子房的1侧着生；花丝不显著；药隔显著，如果雄蕊3，药隔在基部合生或离生；室花药1或2，纵向开裂；雌蕊1，1心皮；子房下位，1室；胚珠1，下垂，；花柱短或无。

Methods Mandibular angle oblique ostectomy by intraoral method. Move the chin forward and downward after horizontal ostectomy,with bone autograft inserted and fixed with Ti screw and plate.

口内入路下颌角斜形截骨，颏部水平截骨向前向下移动，骨片植入。

Once the osteotomy is completed, the guide pin is inserted into the expansion appliance and activated.

一旦截骨完成后，引导针插入扩张设备和激活。

Oxyfluoride materials are composed of fluoride micro-crystal which is inserted into oxide glass host. Rare earth ions were priority deposited into fluoride micro-crystal, with lower phonon energy environment.

氟氧化合物由氟化物微晶镶嵌于氧化物玻璃基质中构成的，掺杂的稀土离子优先沉淀于氟化物微晶中，处于低声子能量的环境。

Methods:One hundred and six patients (118 lunbar discs) whose intervertebral disc center were inserted into percutaneous puncture needle under X-ray fluoroscopy after local anesthesia were treated by semiconductor laser (power 0～15 W, wavelength 980 nm)combined with ozonator. The single interspace total laser energy was controlled at 1 000～1 200 J and the concentration of ozone was 40～50 mg/L.

患者俯卧位，局麻后在透视定位下经皮穿刺进入病变椎间盘中心，应用半导体激光（功率0～15 W，波长980 nm）、医用臭氧发生器联合进行髓核汽化减压及消融，单间隙总能量控制在1 000～1 200 J，臭氧浓度40～50 mg/L，共治疗106例118个椎间盘。

METHODS:(1)The bile-pancreatic ducts of rats were ligated,the catheters were inserted into right jugular vein and jejunum and the acute pancreatitis model was established.Forty pancreatitic rats were divided into four groups and were given simple parenteral nutrition(PN,n=10),early enteral nutrition(EEN,n=10), median enteral nutrition(MEN,n=10),and late enteral nutrition(LEN,n=10) respectively.

(1)结扎大鼠胆胰管，经右颈静脉及空肠插管建立大鼠急性胰腺炎营养支持模型。40只急性胰腺炎大鼠分别给予：单纯肠外营养(PN组，n=10)，早期肠内营养(EEN组，n=10)，中期肠内营养(MEN组，n=10)，晚期肠内营养(LEN组，n=10)。

Follicles 5- to many seeded; antepetalous stamens inserted ca. 2 mm from petal base; seeds papillate.

蓇葖果5-在种子多数；对瓣的雄蕊把约2毫米插入从花瓣基础；种子具乳突。

Shop assistant: Please check at the door.

店员：请您到门口柜台交费。

In the end, transportation of PAHs in the environmental matrix was preliminarily investigated and discussed according to PAHs data of soils and sediments from a certain region in Shanghai. The result indicated that there are two or more pathways on PAHs transportation in the environmental matrix.

最后，对上海特定地区内PAHs在环境介质中的迁移径途进行了分析讨论，结果表明上海市土壤和沉积物等环境介质中PAHs的迁移存在多种途径。