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In fact, because Hymen hole of the circle is very small,great majority female is in after first time sexual intercourse can contain one little toe only commonly, the meeting after the penis is inserted causes Hymen to be in or much place is torn, bring about first sexual life bleeds, later as coital number increase, vaginal mouth also increases gradually, hymen crack also assumes much office or show dated sex loss.

事实上，绝大多数女性在第一次性交后由于圆形的处女膜孔很小一般仅能容入一小指，阴茎插入后会致使处女膜一处或多处被撕破，导致首次性生活出血，以后随着性交次数增多，阴道口也逐渐增大，处女膜裂痕也呈多处或呈陈旧性损伤。

The study also raises doubts about whether "virtual colonoscopy," performed by a CT scanner, will ever be able to take the place of the colonoscope inserted into the rectum, as many patients had hoped.

该研究也增加了这样一种疑问，是否应该如许多病人希望的那样，用CT扫描来取代实质上的结肠镜检查。

Methods 200 patients attending for colonoscopy were randomly chosen and divided into control group(n=100)and mac group(n=100).control group was given normal colonoscopy.mac group was given fentanyl (1μg /kg) and propofol (1～2 mg/s) intravenously.if the patients reached classⅴ of ramsay score,inserted the colonoscope,and maintained with propofol 5～10 mg/min.

200例行肠镜检查者随机分为mac组(n=100)和对照组(n=100)，对照组行常规肠镜检查。mac组以芬太尼1 μg/kg静脉注射，继以异丙酚1～2 mg/s速度推注，ramsay镇静分级5级后行肠镜检查，术中5 ～10 mg/min速度维持mac。

Methods:The total RNA was extracted from mouse RAW264.7 cells stimulated by LPS.The sequence including the whole length of HMGB1 was amplified by RT-PCR and inserted into pMD-19T.The combinant vector was used as a template for PCR which was cloned into vector pMD-19T,then subcloned into expression vector pET-26b with pelB signal sequence and His-Taq sequence.After transforming E.coli BL21(DE3) and four hours induction by IPTG,HMGB1 expression confirmed by SDS-PAGE and the purification was performed by Ni2+-chelate affinity chromatograph.

脂多糖刺激后的RAW264.7细胞，提取总RNA，经RT-PCR扩增出含HMGB1的目的片段，克隆于pMD-19T载体，再亚克隆至含有pelB引导肽及His-标签肽的高效表达载体pET-26b，转化大肠杆菌BL21（DE3），经IPTG诱导后行SDS-PAGE鉴定目标蛋白表达，用镍鳌合琼脂糖凝胶亲和层析法分离纯化含His-标签肽的目的蛋白。

Methods:The total RNA was extracted from mouse RAW264.7 cells stimulated by LPS.The sequence including the whole length of HMGB1 was amplified by RT-PCR and inserted into pMD-19T.The combinant vector was used as a template for PCR which was cloned into vector pMD-19T,then subcloned into expression vector pET-26b with pelB signal sequence and His-Taq sequence.

脂多糖刺激后的RAW264.7细胞，提取总RNA，经RT-PCR扩增出含HMGB1的目的片段，克隆于pMD-19T载体，再亚克隆至含有pelB引导肽及His-标签肽的高效表达载体pET-26b，转化大肠杆菌BL21（DE3），经IPTG诱导后行SDS-PAGE鉴定目标蛋白表达，用镍鳌合琼脂糖凝胶亲和层析法分离纯化含His-标签肽的目的蛋白。

Stamens 4, subequal or anterior 2 longer, inserted on apical half of corolla tube; filaments complanate, glabrous or puberulent; anthers ovoid, cells 2, divaricate, becoming confluent at apex.

雄蕊4，前面的近等长或长，顶端的着生于花冠筒的一半；扁平的花丝，无毛或具微柔毛；花药卵形体，室，极叉开，变得的合流河在先端。

Stamens 4, didynamous, inserted on corolla tube; anther locules divergent, apically connivent.

上唇2裂。雄蕊4，二强雄蕊，着生于花冠筒；药室分叉，顶部靠合。

Stamens inserted at apex of corolla tube; filaments short; anthers sagittate, connivent, adherent to pistil head, spurred at base; disc absent.

雄蕊花冠筒的着生的在先端；花丝短花药箭头形，靠合，对雌蕊头状花序，基部具距附着；花盘无。

Stamens inserted high in corolla tube; anthers often connivent or connate around style, dehiscing by apical pores, often later splitting longitudinally.

在花冠筒里着生高的雄蕊；在花柱周围合生的花药通常靠合的或，通过顶孔开裂，通常过后纵向劈开。

Stamens inserted at apex of corolla tube; filaments short; anthers exserted, sagittate, connivent at middle, adherent to pistil head, cells spurred at base; disc 5-parted.

雄蕊花冠筒的着生的在先端；花丝短花药外露，箭头形，靠合的在中部，对雌蕊头状花序，室基部具距附着；花盘5深裂。

What would he tell Judith and the children?

他将怎样告诉朱迪丝和孩子们呢？

I this is at that time, the opinion with peacockish true girl is full of in a heart.

这就是当时的我，一个心中布满虚荣的女孩子真实的想法。