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In this experiment, His 5 gene was inserted into Ura 3 gene and constructed a new plasmid pLRH33, which contained a complete His 5 gene and partial Ura 3 gene at the ends of His 5 gene.

实验将His 5基因插入到一个Ura 3基因的中间，构建了一个新的质粒pLRH33，从而打断了Ura 3 基因使之不能表达。

After treatment with the above hormones, the rats were established anesthesia with urethan (1g/kg, i. p.); polyethylene catheters were inserted into the external jugular vein and the common carotid artery for drug administration, and then blood pressure was recorded. The arterial catheter was connected to a pressure transducer, and the blood pressure was displayed on the channel of BL-310 Experimental System of Biological Function through IBM computer. We assessed the changes of resting arterial blood pressure, the pressor responses of angiotensin Ⅱ(Ang Ⅱ, 0.01mg/kg), noradrenaline (NA, 0.01mg/kg) and adrenaline (Adr, 0.016mg/kg). The depressor responses of Ach (0.006mg/kg) and carotid sinus baroreflex were also discussed after treatment with the hormones in OVX rats.

用不同雌激素处理21天后，将动物用25％的氨基甲酸乙酯腹腔麻醉（1g／kg），做颈总动脉插管，通过压力换能器，将血压变化输入计算机，通过BL-310智能型生物信号显示与处理系统的压力测定模块，观察去势鼠及不同雌激素替代治疗大鼠静脉注射血管紧张素Ⅱ（AngⅡ，0.01mg／kg）、去甲肾上腺素（NA，0.01mg／kg）、肾上腺素（Adr，0.016mg／kg）的升压反应以及乙酰胆碱（Ach，0.006mg／kg）的降压反应和压力感受性反射的变化。

Petals 4 or 5, inserted near mouth of calyx tube, imbricate or valvate in bud, conspicuous or not, or absent.

花瓣的4或者5，在萼筒的口部附近着生，在芽期覆瓦状或者镊合状排列，明显或不明显，或者无。

In order to detect the biologi cal function of this gene,1197 gene was inserted into expressing vec tor pGEX-3X .

经过一段时间的努力研究后，我们报导了引起该病的病原是一种无包含体的杆形病毒［1］。

Methods: The coding region of superoxide dismutase was amplified using PCR method from the E.co1i genome. The PCR product was cloned into PUC19-T vector and sequenced. In addition, the cloned coding region of Mn-SOD was inserted into the expression vector PET-28a to form the recombinant plasmid PET-28a-Mn-SOD and was then transformed into E.coli BL21 for expression.

用PCR方法从大肠杆菌2号基因组中扩增Mn-SOD基因编码区，克隆到pUCm-T Vector，测定核苷酸序列；再将基因编码区克隆到原核表达载体PET-28a，构建含Mn-SOD基因的重组表达质粒，转化到大肠杆菌BL21中进行IPTG诱导表达。

objective: to achieve the soluble expression of mn-sod gene in e.coli and assay the enzyme activity of the expressed product. methods: the coding region of superoxide dismutase was amplified using pcr method from the e.coli genome. the pcr product was cloned into puc19-t vector and sequenced.in addition,the cloned coding region of mn-sod was inserted into the expression vector pet-28a to form the recombinant plasmid pet-28a-mn-sod and was then transformed into e.coli bl21 for expression.

超氧化物歧化酶(superoxide dismutase，sod)是细胞体内歧化超氧阴离子自由基(o-2)的一个抗氧化酶，按其结合的金属性离子根据其中金属辅基的不同可分为4类：cu/zn-sod、mn-sod、fe-sod和ni-sod，它们通过催化超氧阴离子自由基0-2发生歧化反应，达到清除o-2的效果，具有防御氧毒性、增强机体抗辐射损伤能力、防衰老以及治疗某些肿瘤、炎症、自身免疫疾病等功效，广受国内外科研工作者的关注和重视[1]。

Once inserted into the system, the use of advanced electronic digital signal processing and coherent technology meant that no re-engineering of VERNet's existing fiber routes was needed and the new 40G and 100G wavelength services were active and working immediately.

植入新系统后，高级电子数字信号处理和相干技术的运用意味着VERNet现有的光纤路由不必重新设计，新的40G和100G波长服务马上即可上线运行。

A frame-by-frame address code time reference recorded on the spare track of a videotape or inserted in the vertical blanking interval for editing purposes.

一个逐帧的地址编码时间参照，记录在一盘录像带的备用轨道上，或插入到垂直的空白间隔中以备剪辑之需。

You agreed to see me, and you drove out to the hotel where I wassupervision," he inserted virtuously,"but you tripped on the stairs on

但在来我房间的路上，你失足从楼梯上摔了下来……当然，剩下的部分你都知道了。

The virus sliced into the vole's DNA and inserted the instructions to create more receptors.

该病毒嵌入田鼠的 DNA 并插入创造更多受体的指令。

What would he tell Judith and the children?

他将怎样告诉朱迪丝和孩子们呢？

I this is at that time, the opinion with peacockish true girl is full of in a heart.

这就是当时的我，一个心中布满虚荣的女孩子真实的想法。