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METHODS: After AIF△1-120 gene was successfully cloned, the shorter truncated human AIF gene (AIF△1-400) was constructed by deleting the Nterminal mitochondrial localization sequence, the coding sequence of flavine adenine dinucleotide binding domain and nicotinamide adenine dinucleotide binding domain of AIF protein.The truncated human AIF gene (AIF△1-400) was inserted into the pIRES2EGFP and pcDNA3 eukaryotic expression vectors, and the coexpression vectors were then transfected into HeLa cells with LipofectAMINE.

在AIF△1-120基因克隆成功的基础上进一步改造，构建截去AIF基因线粒体定位信号，FAD结合结构域和NADH结合结构域（1-400位氨基酸）编码序列的AIF△1-400基因，将其克隆入pcDNA3和pIRES2EGFP真核表达载体，用脂质体法转染HeLa细胞，通过荧光显微镜观察，MTT检测等方法检测该基因在转染细胞中的表达及其对肿瘤细胞的促凋亡活性。

After tweaking to make it just right, it was put on a floppy disc and inserted into a computer-controlled mill to cut a template.

经过调整，使之恰到好处，这是把软盘和光盘插入到计算机控制的工厂削减一个模板。

Receptacle 1. The tip of the angiosperm flower stalk upon which the floral organs are inserted.

花托：被子植物花茎顶部花着生的部位。

I hurriedly inserted it in to a flowerpot.

我慌忙将它插到花盆里。

A long, thin guide wire with a smooth rounded tip is first inserted and then guided, under X-ray control using a fluorescent screen or a TV image intensifier, into the vessel to be examined.

长期以来，薄导丝与顺利四舍五入秘诀是第一次插入，然后引导下， X射线控制使用荧光屏幕或电视图像增强器，到船只加以研究。

When a capillary tube, in whose inner hole an optical fiber has been fixed and whose axial deviation amount between an outer peripheral surface of the capillary tube and a core center of an end surface of the optical fiber is 1.5 mum or less, is inserted into the insertion portion of the thin tube and the end surface of the optical fiber is fixed at a position at which a distance of the end surface to a focal point position of the partially spherical lens becomes -40 mum or less, emission light has an emission light bend of 0.2 DEG or less with respect to the center axis of the thin tube.

当将光纤固定在内孔且外周面与光纤的端面的纤芯中心的轴偏移量在1.5μm以内的毛细管插入细管的插入部，并且将光纤的端面固定于部分球面透镜的焦点位置±40μm以内的距离的位置上时，出射光曲率为出射光相对细管中心轴在0.2°以内。

A leaf or group of pages inserted into a publication.3. A foldout, especially one that opens to double the page size.

折叠插页一张插页，尤指打开的使纸张的尺寸扩大为原来的两倍的插页

Foretype's guess is shown as a tooltip, which can be inserted by pressing either the Tab or Enter keys.

典型的揣测是显示一个可以被通过按回车键或者跳格键插入的工具提示。

Contains the channel sink provider for a formatter sink that is to be inserted into the channel sink chain.

包含要插入信道接收器链的格式化程序接收器的信道接收器提供程序。

Coby Karl was inserted with 3:19 to play and made a technical free throw in front of Denver's bench.

科比·卡尔上场3'19''，并在掘金的提不上上博得一次技术犯规。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。