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The difference between the test groups and the control group was statistically significant. It could be deduced that all the insecticides tested in this experiment damaged the spiders′ intestinal mucosa, compromising its function as a barrier and letting large molecules such as FTIC-D more easily penetrate. Among the insecticides, pyrethrin and Bt preparation are bio-insecticides, and the FITC-D concentrations of the spiders exposed to these two bio-insecticides were more than those of the spiders exposed to the chemical insecticides imidacloprid and dipterex. The difference between the bio-insecticide groups and the imidacloprid group was significant, but there was no significant difference between the bio-insecticide groups and the dipterex group.

结果表明:4种杀虫剂处理后拟环纹豹蛛血液中的荧光素浓度远高于对照组,各个处理组拟环纹豹蛛血液样本中的荧光素浓度均与对照组存在极显著差异,可见4种杀虫剂均将对拟环纹豹蛛肠黏膜的屏障功能造成损伤,导致大分子物质易于通过;其中1.5%除虫菊素水乳剂三保奇花和Bt制剂强杀处理后拟环纹豹蛛中肠黏膜的通透性高于仿烟碱类化学杀虫剂吡虫啉处理组,差异达到显著水平,但与有机磷杀虫剂敌百虫处理组差异不显著。

Effects of four insecticides on intestinal mucosa permeability of the wolf spider were studied by exposing spiders to insecticides and then measuring the concentration of fluorescein isothiocyanate-dextran in the spiders'blood. The results showed that FITC-D concentrations of the test groups were much more than that of the control group.

采用异硫氰酸荧光素-葡聚糖作为示踪物质,通过检测拟环纹豹蛛血液内的荧光素浓度,研究了两种生物源杀虫剂和两种化学源杀虫剂对拟环纹豹蛛肠黏膜通透性的影响。

The 9 common used insecticides of cypermethrin 4.5%EC, omethoate 40%EC, chlorpyrifos 40%EC, acetamiprid 3%EC, imidacloprid 10%EC provided excellent control for wheat aphids in fields. The control effects were over 90% at 7 days after spraying. The 9 insecticides had bad effects on aphidius and coccinellidae in three days after spraying and the number of two natural enemies decreased rapidly. But on the 7th day, the number of the two natural enemies increased and there were difference between those treated with imidacloprid, acetamiprid and abamectin and CK. BT had no evident difference compared to the control.

为了明确杀蚜药剂对麦蚜的防治效果、适宜的防治时期、对生态环境安全性和粮食作物食品安全性的影响,对4.5%氯氰菊酯、40%氧化乐果、40%毒死蜱、3%啶虫脒、10%吡虫啉等9种防治麦蚜的药剂进行了筛选,发现各药剂对麦蚜的防效较好,喷药后7d防效均在90%以上。9种农药在喷施后的3d内,对麦蚜的主要天敌蚜茧蜂和瓢虫影响较大,两种天敌数量锐减,但到7d时各处理蚜茧蜂数量均有回升,其中不同剂型的吡虫啉、啶虫脒和鲁植顺克处理的蚜茧蜂数量同对照相差不大。

Toxic effect of insecticides on Coccinella septempunctata was studied by two design methods of direct and indirect effect with 8 usual insecticides in apple production as materials.

方法]以苹果生产中常用的8种杀虫剂为材料,采用直接作用和间接作用两种设计方法,研究了杀虫剂对七星瓢虫的毒杀作用。

The lower dosage of commonly used insecticides mixed with Piperonyl Butoxide may have better control efficacy after the resistance of mosquitoes to insecticides was produced.

当淡色库蚊产生抗药性后,采用杀虫剂与氧化胡椒基丁醚混用的方法,可以减少杀虫剂的使用剂量,取得较好防治效果。

Objective The toxicity and synergism of several commonly used insecticides mixed with Piperonyl Butoxide against the larvae of Culex pipiens pallens collected from fields of three regions in Shandong province were determined for controlling vector mosquitoes by scientifically rational use of insecticides as well as delaying and overcome the occurrence and development of resis-tance.

目的 了解常用杀虫剂与增效剂氧化胡椒基丁醚(S 1 )混用对山东省三个不同地区现场淡色库蚊的毒力及增效作用。

The results showed that 3 acaricides, pyridaben, propargite, hexythiazox and 6 insecticides, abamectin, chlorfenapyr, cyhalothrin, emamectin benzoate, oxymatrine prosuler, envidor had good control effects and there was no significant difference among them. It had excellent control effects for 4 low-toxicity insecticides including propargite, hexythiazox, abamectin, and emamectin benzoate.

结果表明,3种专一性杀螨剂哒螨灵、克螨特、噻螨酮及6种广谱性杀虫杀螨剂阿维菌素、虫螨腈、高效氟氯氰菊酯、甲维盐、氧苦内脂和螺螨酯对叶螨均有良好的防治效果,彼此之间差异不显著。

Methods The method of the residual film in glass tube with 3 h exposure to insecticides was employed for determining toxicity of insecticides to both wheat aphid species, which were originally collected from different provinces of China during 2005-2007 and reared on wheat seedlings in the laboratory for more than 30 generations.

方法]从田间采集麦蚜在实验室内饲养30代以上,利用玻璃管药膜法测定其对杀虫剂的敏感度,每条毒力基线为2次以上独立测定数据合并后的计算结果。

Using a long-term reared housefly population which is very susceptible to pyrethroid insecticides as the susceptible strain, a population collected in 1993 in Shanghai and has been treaded by cyhalothrin as the resistant strain, we bioassayed their susceptibility to organophosphorus, carbamate, pyrethroid and organehloride insecticides. We also investigated the role of metabolic enzymes, i. e. esterase, GST, MFO and of target enzymes, i. e. AChE and 〓-ATPase in resistance to insecticides. Cyhalothrin resistance inheritance, the co-toxicity coefficient of cyhalothrin and methylene dithiocyanate was also studied. The insecticides resistance in adults and larvae of houseflies was compared and the mechanism of resistance differences between adults and larvae was discussed.

本文以采自野外并经过室内多代筛选的三氟氯氰菊酯抗性家蝇品系和室内多年饲养的敏感家蝇品系为试虫,测定了抗性家蝇品系和敏感家蝇品系对拟除虫菊酯杀虫剂三氟氯氰菊酯、氯菊酯、有机磷杀虫剂DDVP、氨基甲酸酯杀虫剂残杀威、有机氧杀虫剂DDT的抗性水平,并通过活体增效剂实验和离体生化实验研究了抗性品系的解毒酶和靶标酶的变化,本文还研究了三氟氯氰菊酯和增效剂I对抗性家蝇的增效作用及增效机理、家蝇幼虫和成虫对杀虫剂的抗性差异及其抗性差异的机理。

Based on the above research, The situation and level of insecticides resistance in the German cockroach, relationship between activity of P450s and resistance, cloning and identification cytochrome P450 CYP4 genes, phylogenetic relations of insect P450 and the role of CYP4 genes in insecticides resistance of the German cockroach were clarified. The methods to analysising early diagnostics of resistant colony was established.

通过上述研究工作,基本上阐明了德国小蠊的抗药性水平、抗药性的产生和发展、抗性群体的早期诊断、多功能氧化酶系的环氧化活力与抗药性的关系、德国小蠊CYP4基因的克隆和核心序列分析、昆虫P450基因的同源性及进化、CYP4基因在德国小蠊抗药性中的作用等几个方面的内容,并建立了抗药性的早期诊断技术。

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