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The plasmid pcDNA3.1-3-1E and pcDNA3.1 was respectively used to immunize SPF chicks by injection intra-muscularly in thigh muscle at 14 and 21 days of age.Chicks in each group except that in the unchallenged control group were challenged with 5×104 E.acervulina sporulated oocysts at 28 days post-inoculation,and the immune protective efficacy was observed.

将构建的重组质粒以及空载体质粒分别于14、21日龄分2次经腿部肌肉注射免疫SPF雏鸡，28日龄除非免疫非感染对照组外各组攻击性感染5×104个堆形艾美球虫孢子化卵囊，观察真核表达质粒对球虫感染鸡的免疫保护作用并探讨其免疫保护机理。

Ten 10-day-old artificially-reared coccidian-free goslings were inoculated orally with 2.0×105-6.5×105 of sporulated oocysts of Eimeria stigmosa,respectively, and killed at intervals from 48th h to 180th h post-inoculation.

给10只10日龄雏鹅分别经口接种2.0×105~6.5×105个多斑艾美球虫孢子化卵囊，在接种后48~180h分期剖杀，对多斑艾美球虫的生活史和致病性进行了动态观察。

The research on the choice of bacteria vaccine and its addition, improvement of preparation and fermentation technique suggests that the amino nitrogen level can reach that of 180 at the time of only 60 days in artificial inoculation and the production period can be shortened to two thirds.

对豆酱制曲菌种的选择、制曲工艺条件的优化、种曲添加量及发酵工艺的优化研究表明，多菌种人工接种生产豆酱中氨基态氮的含量在第60天时就达到了自然发酵180天时的含量水平，生产周期可缩短三分之二。

The results showed that the optimum condition for straw anaerobic fementation is the straws was 8cm length and pretreatment with 10% ammonia liquor, inoculation proportion 1:1.5, control the humidity of sludge 91.7%, and the culture temperature 35℃, with supplement of 0.5% urea.

结果表明农作物秸秆厌氧消化产甲烷的适宜条件是：用质量分数10%的氨水预处理，秸秆长度8cm，接种量1：1.5，接种污泥含水率91.7%，反应温度35℃，尿素添加量为秸秆用量的0.5%。

At difierent time at ter inoculation with Fusarium oxysporum f.sp.cubenserace 4,the transcript pattems of WNB1 and WST3 was enhanced,which implied that the expression of WNB1 and WST3 may be related to the resistance of banana to Fusarium wilt.

用半定量PCR分析枯萎病菌诱导后野生蕉叶片中RGAs的表达情况，结果表明WNB1和WST3受枯萎病菌诱导后表达量增强，这表明WNB1和WST3的表达可能与香蕉枯萎病抗性相关。

The mycorrhizal formation,inoculation effectiveness,extraradical fungal biomass and P uptake contribution of six arbuscular mycorrhizal isolates screened from South China,Center China and North China on typical cinnamon soil,brown soil and red soil collected in above three regions were investigated using three-compartment glass-bead culture system associated with maize.

以分离于华北、华中和华南3个生态区及法国引进的丛枝菌根真菌为试验菌株，采用三室根箱培养的方法，研究了它们在华北、华中和华南3种典型土壤—褐土、棕壤和红壤上的菌根形成、接种效应、磷吸收贡献和根外菌物量情况。

The inoculation effectiveness of fungi varied with soil types,especially for BEG167.BEG167 performed positively on cinnamon soil,and neutral on brown soil,but negatively on red soil.Except for BEG150 all All other fungi,except BEG150,increased maize P uptake on cinnamon soil and brown soil,however,only BEG168 and BEG 141 improved P uptake significantly on acidic soil.

若以真菌的根外菌物量作为衡量AM真菌菌株土壤生态适应性的指标，BEG141和BEG167是生态适应性强的菌株，为广幅生态型菌株；菌株BEG168和BEG151次之，前者在棕壤上适应性高，后者在红壤上适应性高。

In order to improve the nutrition level of cottonseed cake power and reduce the content of dissociate free gossypol,solid fermentation was adopt to research the influence factor of solid cottonseed cake fermentation such as Strains、carbon sources and original pH,quantity of inoculation,fermentation time,fermentation temperature.

摘 要：为了提高棉籽饼粉的营养水平及降低游离棉酚的含量，采用固体发酵法，对棉籽饼粉固体发酵的影响因素如：菌种、碳源、以及pH值、接种量、发酵时间、发酵温度进行了研究。

The results showed that the free gossypol was reduced 52.6%, the protein was increased 5.97% and free amino acids was increased 181.1% when the fermentation Strains was Mucor,the proportion of cottonseed cake to wheat bran 6:1, solid to liquid 1:1, original pH 6.0,quantity of inoculation 5%,the fermentation temperature 25℃, the culture time 4 d.

结果表明：以毛霉为发酵菌种；棉籽饼：麦麸为6：1，固液比为1：1，初始pH值为6.0，接种量为5%，发酵温度为25℃，发酵时间为4d。游离棉酚含量降低了52.6%，蛋白质含量提高了5.97%，游离氨基酸含量提高了181.1%。

RESULTS:①Growth and adhension of osteoblasts: 24 hours after inoculation, osteoblasts were fully adhered to the wall in the BPCB group and cover slip group.

细胞在材料上的生长及附着：随着培养时间的延长，附着于材料表面和孔隙的细胞数量逐渐增多，并向孔隙内部长入，细胞形态以多角形为主。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。