查询词典 inoculation

Two organisms(SG1,SG2)were isolated from the affected birds,which were identified as Chlamydia psittaci by means of electromicroscopic observation,artificial culture media inoculation test,sulfanilamide sensitivity test and iodine staining.

从发病鸽分离到 2株菌(SG1、SG2 )，经电镜形态学观察、人工培养基接种试验、碘胺敏感性试验及碘染色试验鉴定为鹦鹉热衣原体。

The first passage cells were initially pured by enzyme digestion, then pured by enzyme digestion and repeated strapping alternatively in the second course of transferation and inoculation, the third period cultured cells showed typical clonali growth and were long fusiform fibroid cells with short growth cycle.

首次传代时应用胰酶消化法进行成纤维细胞的初次纯化，第2次转种时先后应用酶消化法和反复贴壁法再次进行细胞纯化，第3代以后基本可获得呈典型克隆性生长、长梭形纤维样细胞，生长周期短。

Results No post-inoculation pyrogenetic reaction were observed in both trial group and control group, the local reaction rates being 1.8% and 2.1%（P〉0.05）, respectively, indicating slight reactions.

方法按整群随机抽样原则，以进口同类疫苗作为对照开展现场临床试验；比较两种疫苗免疫后临床反应率、抗体阳转率、保护率及几何平均滴度。

Rechallenge was performed 60 days after first tumor inoculation, all mice showed complete protection, indicating that the DC-gp100 induced specific anti-melanoma memory CD8+CTL.

为了验证NV抗原表位可以降低gp100特异的CD8+T 细胞活化阈值，我们先将NV抗原肽负载DC，接着再分别负载不同浓度梯度的gp100抗原肽，以此免疫小鼠，7天后以淋巴细胞增殖实验判断gp100特异的CD8+T 细胞活化阈值。

If the second inoculation follows the previous one is less than 10 years, it is valid for 10 years from the day of reinoculation.

如果前次接种不满 10年又经复种，自受种的七日起，10年内有效

If the second inoculation follows the previous one in less than 10 years, it is valid for 10 years from the day of reinoculation.

如果前次接种不满十年经复种，自复种的当日起，十年内有效

If the second inoculation follows the previous one is less than 10 years, it is valid for 10 years from the day of reinoculation.

黄热病疫苗自接种后第10日起，10年内有效。

METHODS:The gene of human ET1 was synthesized according to the preferential codons of E. coli, cloned into the EcoRI and SalI sites of vector pThioHisA. The recombinant plasmid pThioHisA-ET1 was constructed , sequenced and transformed into E.coli TOP10. Induced and expressed fusion protein were identified and analysed by 12% SDS-PAGE and densitometry analyses. After the elution, denature and renature, the fusion protein Thioredoxin-ET1 was obtained by ProBondTM chromatogragraphy. The purity of Thioredoxin-ET1 was detected by HPLC. Inoculate Thioredoxin-ET1 once per mouse every 2 weeks in 25, 50 and 100μg separately on 3 groups for 4 times. 10 days after last inoculation, we obtained venipuncture blood.

根据人ET1的多肽序列合成ET1基因，将其插入到pThioHisA的EcoRI和 SalI位点，重组质粒pThioHisA-ET1进行酶切鉴定及序列测定验证后转化TOP10，IPTG诱导的重组菌经SDS-PAGE检测融合蛋白Thioredoxin-ET1的表达量；表达的融合蛋白用ProBond亲合层析纯化并经HPLC鉴测其纯度；每只小鼠按25、50、100ug/次剂量的Thioredoxin-ET1每两周免疫一次，共4次，最后一次免疫10d后制备抗血清，经Western blot和ELISA检测证明Thioredoxin-ET1融合蛋白具有ET1免疫反应原性。

The optimum technological parameters were the ratio of beer Saccharomycete to Lactobacillus plantarum 1︰2, the quantity of inoculation 0.9%, the fermentation temperature 28℃, and the fermentation time 24 h.

了降低牛肉香肠中的胆固醇含量，采用饱和D-最优试验设计方法优化香肠的工艺参数，以胆固醇降解率和感官评定为指标值，通过回归分析建立目标函数的数学模型，从而确定最优加工工艺参数为：啤酒酵母菌、植物乳杆菌配比为1︰2，接种量为0.9%，发酵温度为28℃，发酵时间为24 h。

The study on the reducing browning of callus induced from Aloe saponaria Haw indicated that: pretreatment before inoculation was useful for reducing callus browning.

对降低皂质芦荟诱导愈伤组织褐化情况的研究表明，外植体接种前进行预处理对控制诱导愈伤组织的褐化有一定的帮助。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。