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Albopictus had mesenteronal infection barrier or mesenteronal escape barrier after inoculation perorally with dengue type 2 virus.

当用不同剂量感染时，白纹伊蚊的感染阳性率和感染病毒剂量呈正相关。

This research studied the optimal strain combination through single strain and mixed strains fermentation from Sweet Sorghum juice. The suitable proportion and quantity of inoculation, the optimum nitrogen source and its ratio in the medium were established through mixed fermentation. The optimum pH values, fermentation time and temperature were obtained by L9 (3)3 orthogonal experiment. The optimum mixture of biotechnology fulvic acid, Azotobacter chooroccum, Phospho bacteria and Silicate bacteria were studied.

本文以甜高粱秸秆汁为原料，通过单菌及混合菌种发酵实验确定了发酵的最佳菌种组合；利用微生物混合发酵确定了发酵菌种的接种比例、接种量、最佳氮源及其添加量；用L_9(3)~3正交设计的方法，确定了发酵的最佳pH值、发酵时间和发酵温度；研究了生化黄腐酸液体肥料和圆褐固氮菌、钾细菌、磷细菌的复配工艺，并在作物上进行了田间试验。

the condition of ph 8.0, the intension of illumination 1.0 klx, the time of continuous cultivation 24 hours, the temperature 24 ℃ and the density of inoculation 1.95×105ml-1 are fit for haematococcus pluvialis's fast growing.

笔者从ph值、光照强度、接种密度、温度等因素研究雨生红球藻快速繁殖生长的最适宜条件，为大规模培养雨生红球藻摸索条件。

After natural disasters, polymicrobial infections may occur following heavy inoculation during trauma.

灾后，多种病原微生物感染可能发生在外伤期间大量的预防接种之后。

M treatment materials, and also tartaric acid and no maleic acid detected in G. v treatment materials. Inoculation also increased the content of organic acid secretion (increased by 19.80～56.87 mg/kg) of trifoliate root, and the application of AlPO4 greatly increased organic acid content (increased by 20.06～21.84 mg/kg); uninoculated plant root secreted a bit of organic acid; Mycorrhizal poncirus trifoliata seedlings excreted malic acid (42.87%), citric acid (39.22%) and oxalic acid (12.06%).

接种AM真菌的根系分泌的有机酸种类与对照有所不同，未接种处理枳分泌的有机酸有草酸、苹果酸、乳酸、乙酸、顺丁烯二酸和柠檬酸等6种，而接种G.m的则检测到草酸、酒石酸、苹果酸、乳酸、乙酸、柠檬酸、丁二酸等7种，G.v处理的检测到酒石酸，接种处理均未检测到顺丁烯二酸；接种丛枝菌根真菌增加了枳根系分泌有机酸的量比未接种处理增加19.80～56.87 mg/kg，且施用AlPO4后有机酸含量显著增加(增加20.06～21.84 mg/kg；未接种植株根系仅分泌少量有机酸；接种植株根系分泌的有机酸以苹果酸(42.87%)、柠檬酸(39.22%)和草酸(12.06%)为主。

The effects of variety preculture time, activate medium of A.tum, concentration of Ca, inoculation time, treated with AgNO3 and PVP or not, delay selection time on transformation frequency were studied as well. The results demonstrated that using 20Omg.LCef, 8 mg.L1Kam.

结果表明，使用200mg.L~(-1)Cef、8mg.L~(-1)Kam、预培养2天、使用GCJ8活化培养基、在预培养和共培养基中使用5倍Ca~离子浓度、侵染1分钟、使用4mg.L~(-1)AgNO3和0.7％PVP，延迟筛选5天时取得了最高的转化频率。

The results showed that, using transient GUS expression, the frequency of resistant calli and shoot differentiation as a criteria, wheat mature embryos were cultured on the preculture medium of CM4C1P for 14 days, and immersed in inoculation suspension Ⅰ for 3 hours, then the explants were co-cultured under desiccation conditions for 3 days, which was optimal conditions for Agrobacterium-mediated transformation of wheat mature embryos.

为了进一步完善根癌农杆菌介导的小麦遗传转化体系，以小麦成熟胚为转化受体，研究了根癌农杆菌介导法转化小麦的主要影响因素，结果表明，以gus基因瞬时表达率、抗性愈伤组织获得率和分化率为转化指标，小麦成熟胚在预培养基CM4C1P上预培养14d后，用侵染培养液Ⅰ侵染3h，再经干燥共培养方式处理3d，是根癌农杆菌介导成熟胚转化的合适条件。

The results showed that: the explants of strawberry leaves were rather sensitive to kanamycin, and the corresponding concentration of 20 mg/L could substantially inhibit the growth of non-transformed tisse and be used to screen the leaves of strawberry after transformation. Timentin at the concentration of 300 mg/L could be used to eliminate Agrobacterium tumefaciens and had minor impact on the growth of the explants. The preculture time of 2-4 days was beneficial to improve transformation frequency. Co-culture for 2-3 days not only had higher gus transient expression but also avoid the over-growth of agrobacterium. The bacterial concentration of OD(subscript 600nm) 0.4 was the best inoculum density and 10 minutes was the best inoculation time.

结果表明：草毒叶片外植体对卡那霉素较为敏感，适宜的筛选浓度为20mg/L，可明显抑制非转化组织的生长；300mg/L的羧吩青霉素可有效抑菌，对外植体生长影响也较小；预培养2-4d有利于转化；共培养2-3d有利于提高转化频率并避免了农杆菌的过度生长；OD(下标 600nm)值为0.4的菌液侵染10min效果最佳。

The preculture time of 2-4 days was beneficial to improve transformation frequency.Co-culture for 2-3 days not only had higher gus transient expression but also avoid the over-growth of agrobacterium.The bacterial concentration of OD_(600nm) 0.4 was the best inoculum density and 10 minutes was the best inoculation time.

结果表明：草莓叶片外植体对卡那霉素较为敏感，适宜的筛选浓度为20mg/L，可明显抑制非转化组织的生长；300mg/L的羧吩青霉素可有效抑菌，对外植体生长影响也较小；预培养2-4d有利于转化；共培养2-3d有利于提高转化频率并避免了农杆菌的过度生长；OD600nm值为0.4的菌液侵染10min效果最佳。

GCJ8 activate medium, preculture 2 days, 5 X Ca concentration in preculture medium and coculture medium, inoculation I mineture,4mg li1 AgN0 and 0.7% P\P, delay selection 5 days will obtain the highest transformation frequenc Using 'pingyitiancha'shoot apex, mediated by A.tum as above system, the HVAIgene, was transformed into pingyitiancha and obtained Kam resistance rerzeneration plants, it is confirmed that 6 plants of them is transformation plants by identified by PCR and dot blot

按照上述体系，以平邑甜茶茎尖分生组织为受体，将HVA1基因导入平邑甜茶中，获得了具有Kam抗性的再生植株，经PCR与点杂交鉴定，证实有6株为转化植株。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。