查询词典 inoculation

The digestion process includes the following steps: adding 0.15 % concentration collagenase II solution compounded with DMEM into blood vessel smooth muscle tissue block and acting in water bath for 1.5 hr until forming floccular tissue; cleaning with 0.02 % EDTA solution, adding 0.125 % trypsase solution and water bath vibrating to digest at 37 deg.c to obtain dispersed smooth muscle cells; terminating enzyme action with 10 % new born ox serum culture liquid; regulating cell concentration with 20 % new born ox serum culture liquid to 50,000-500,000/L, inoculation, and culturing in culture tank with 5 % CO2 and first 37 deg.c to cell fusion of 70-80 %; and subculturing conventionally.

向血管平滑肌组织块中，加入用DMEM配制的0.15％胶原酶II溶液，水浴箱中作用1.5h，至组织呈絮状，用0.02％的EDTA清洗，然后，加入0.125％胰蛋白酶溶液，于37℃水浴振荡消化，即可得到分散的平滑肌细胞，用10％新生牛血清的培养液终止酶的作用。加入含20％新生牛血清的培养液，调节细胞密度为5×104～5×105个/L，接种，放入5％CO2、37℃培养箱中培养。当细胞达到70％～80％融合时即可按常规方法传代。

The main research contents in this thesis mainly study its flocculently active: separating the flocculently active microbial strain of EM and the best inoculation quantity of EM and flocculant character of EM.

本文对EM的絮凝特性进行了以下两方面的研究：1通过增殖培养和定向培养试验，筛选出EM中絮凝剂产生菌；2通过絮凝试验，探讨筛选出絮凝剂产生菌絮凝剂合成的影响因素和絮凝能力的影响因素。

In this research, we have studied the effects of a series of factors (genotypes, basicmedia, plant growth regulators, explants, inoculation methods, leaf ages and darkperiod) in the inducing adventitious shoot of strawberry(Fragaria ananassa Duchcv.

本试验以草莓品种&丰香&和&章姬&为试材，研究了草莓离体再生过程中基因型、基本培养基、植物生长调节剂配比、不同外植体、接种方式、苗龄和暗处理对草莓离体再生不定芽的影响，获得了草莓高效的离体再生体系。

Scab resistance, agronomic traits and their improvement effects of 4 wheat gene pools were studied with different recurrent selection populations under single-floret inoculation with Gibberella zeae.

用长期库LP和抗源库RP的C_1、C_4，近期库GPⅠ和CPⅡ的C_0、C_3轮选群体，在赤霉病单花接种条件下，对其3轮选择前后的抗赤霉病性和10个农艺性状的表现及具改良效果进行了分析。

Gibel carp were inoculated with formalin killed Aeromonas hydrophilaand fed with diets supplemented with appropriate amount of yeast β-glucan. 28 days after the inoculation, the infulence of dietary yeast β-glucan on the resistance of gibel carp against Aeromonas hydrophila were determined by examing their gained weight, agglutinating antibody titer, lysozyme activity, phagocytic activity and relative percent survival by challenging with live A.

在饲料中添加定量的酵母β-葡聚糖，投喂经注射接种福尔马林灭活的嗜水气单胞菌(Aeromonas hy-drophila)菌苗的异育银鲫28 d后，通过测定供试鱼的增重量、血清中凝集抗体效价、溶菌酶活性、谷丙转氨酶活性、血清总蛋白含量、白细胞吞噬活性以及活菌攻毒后的免疫保护率，探讨了酵母β-葡聚糖对受免异育银鲫免疫应答的增强作用。

So it is held that inoculation of bean crops with appropriate rhizobium is an approach to further promoting the merits of N nutrition of the legumious/gramineous intercropping system.

因此，本研究认为豆科作物接种合适的根瘤菌，是进一步提高豆科/禾本科作物间作系统氮营养间作优势的又一重要途径。

The higher the inoculation concentration of the strain is during seed germination, the more quantity the strain in host plant tissues. When strain B504-1121 was inoculated on lower petioles, it could transfer into upper tissues, however it only habitate in leaves if it was inoculated on leaves.

在下部叶片叶柄处接种的菌体可向上移动，通过茎进入上部叶片；在叶部接种的菌，则只在接种叶内定殖，不再向其他部位转移。

The BLSAg appeared in the spleen,the tonsillia cecalia and thymus,the bursa Fabricius,the red marrow and Harderian's gland at the lst week,7th week and 19th week post inoculation.

BLSAg阳性淋巴细胞早期主要分布在胸腺和外周免疫器官的T 淋巴细胞区，第 7周在胸腺、法氏囊和外周免疫器官的T、B淋巴细胞区。

Inoculation of leaf on Helminthosporium Maydis Nisk and observation of anther and pollen and fertility restoration-retention identification indicated that JnA should be classified into group S of cytoplamic male sterility. At the same time, fertility observation of F2 and microscopy identification of F1 pollen through I-KI dyeing confirmed this conclusion.

首先对玉米细胞质雄性不育系JnA进行小斑病菌接种鉴定，同时对其花药、花粉形态观察，通过恢保关系鉴定，F2育性分离观察及F1花粉碘液染色镜检，初步确定JnA属于S组细胞质雄性不育。

To evaluate the efficacy of the recombinant fowlpox virus vaccine against the heterotype IBV, the chickens were challenged with the homotype IBV LX4 strain and the heterotype IBV LTJ95I strain after three weeks post vaccination. Antibodies against IBV were detected in vaccinated chickens one week post inoculation. The CD4+ T-lymphocytes in the peripheral blood increased rapidly in all groups challenged with IBV, except for the vaccinated group challenged by heterotype strain and the low level of CD4+ T-lymphocytes remained until end of the experiment. In all the groups, a high level of CD8+ T-lymphocytes only was observed in the vaccinated group after challenging with IBVhomotype strain. The morbidity and the mortality of this group were 21.43 % and 0 %, respectively, which showed significant difference with other groups. In addition, the lesions of chickens and virus shedding were less in the vaccinated group challenged by IBV homotype strain comparing with other groups, but there was no difference for the average body weight of chickens in all groups.

结果显示，重组疫苗接种1周后，免疫鸡产生抗IBV的抗体；而且外周血中CD4+和CD8+ T淋巴细胞的含量略高于非免疫对照组；攻毒后，异源强毒株攻毒的免疫组CD4+ T淋巴细胞呈下降趋势，并且该组低水平CD4+的状态一直持续到试验结束，而其他组CD4+ T淋巴细胞均迅速上升，峰值达到14.5 %；同源强毒株攻毒的免疫组CD8+ T淋巴细胞呈高水平的表达，而其他组攻毒后均无明显变化；保护率结果显示，同源强毒株攻毒免疫组的发病率和死亡率为21.43 %和0 %，与其他各组相比均有显著差异；另外同源强毒株攻毒的免疫组病理损伤与异源强毒株攻毒的试验组相比明显减轻，其排毒时间和排毒量也均有所减少；强毒攻毒后所有试验组体重无显著差异。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。