查询词典 inoculation

The following methods were used in the study, such as viral inoculation of animal, pathological method, flow cytometry, RT-PCR, in situ hybridization, immunohistochemistry and terminal deoxy nucleotidyl transferase mediated dUTP nick end labling. Morphological changes of the infected chickling, dynamic changes of T, B cells and T subsets, changes of adhesion molecules on the lymphocyte surface, necrosis and apoptosis of lymphocyte and neurons, changes of MIP-1β and IL-8 producing cells in the brain, cell types of perivascular tuffing in cerebral tissue were systematically studied. The key research results were.(1)The average percentage of CD19+cell in blood, bursa and spleen, after 3, 5 days of inoculation, was significantly Phybridization revealed that the number of IL-8 and MIP-1βproducing cells was increased in the infected brain.

在研究过程中，采用了病毒接种技术、普通病理学研究方法、流式细胞仪技术、RT-PCR技术、原位杂交技术、免疫组化技术、凋亡细胞末端标记技术，系统研究了不同日龄的SPF雏鸡人工感染AEV-NH937株后的病理变化，T和B淋巴细胞、T淋巴细胞亚群的动态变化规律，淋巴细胞表面某些粘附分子的变化，淋巴细胞和神经元的坏死与凋亡，雏鸡脑组织中产生趋化因子MIP-1β和IL-8细胞的变化规律，脑组织中围官性细胞浸润的细胞类型。

Furfur. At the same time, selected the culture supernatant. Used the whole-fat-cow"s-milk plate method, the Folin-phenol method and the QuantiCleave proteinase Assay Kit to test the proteinase activity of mycelial phase and yeast phase of M. furfur. The effect of temperature and pH on this assay was also evaluated.Result:①The whole-fat-cow"s-milk plate method: Seven days after inoculation, the proteinase activity of the mycelial phase supernatant (12.5 ± 1.2mm) is higher than that of the yeast phase (9.25±0.71mm)(p.05); With the passage of the inoculation time, the proteinase activity of the mycelial phase is rising; the temperature has no obvious effect on the proteinase

结果：①全脂牛奶平板法：培养第7天菌丝相上清液蛋白酶活性(12.5±1.2mm)高于酵母相(9.25±0.71mm)(p＜0.05)；菌丝相上清液蛋白酶活性随培养天数延长而增大；25℃和37℃两种温度对上清液蛋白酶活性无明显影响(P＞0.05)；②Folin-酚法：上清液蛋白酶活性随培养天数、菌落计数、菌丝诱导率增加而增大，培养液pH值呈下降趋势；同一天的菌丝相上清液蛋白酶活性明显高于酵母相(p＜0.05)；三种温度(25℃、37℃、49℃)及三种pH值(3.8、5.6、7.4)对上清液蛋白酶活性无明显影响；温度37℃、pH5.6时菌丝相上清液蛋白酶活性最高；③QuantiCleave蛋白酶试剂盒：菌丝相及酵母相上清液中均含有蛋白酶且

The results showed that the occurrence of Fusarium wilt was earlier when treated with the root exudates of Jinyan 4, and the infection rate was significantly higher at 15 days after inoculation, but nearly the same as the control at 20 days after inoculation.

结果表明：感病品种根系分泌物处理的黄瓜枯萎病发病早，接种后第15天病株率显著高于对照，至第20天时病株率与对照相近；而抗病品种根系分泌物处理的病株率一直显著小于对照。

The inoculation method chick embryo inoculation, isolation and culture of virus solutionApplication Test hemagglutination test and hemagglutination inhibition.

采用鸡胚接种法中的尿囊腔接种法，分离培养提供的病毒液，应用血球凝集试验和血凝抑制试验检测。

The optimal fermentation process was found through detecting the contents of biotechnology fulvic acid. Results The optimal fermentation process was that inoculation 2%, the inoculation proportion of Candida tropicalis, Pseudomonas and Candida utilis as 1:2:2, 2.0% urea added in Jowar juice, pH 6.0, fermenting 72h at 30℃. The content of fulvic acid could reach more than 20%.

结果 确定了甜高粱秸秆汁发酵生产生化黄腐酸液肥的最适工艺条件：即接种量2%，热带假丝酵母、假单胞杆菌与产朊假丝酵母接种比例为1：2：2，在甜高粱秸秆汁中加入2.0%的尿素，pH值为6.0，发酵温度为30℃，发酵72h，黄腐酸含量达20%以上。

In the condition of the above mentioned, field experiments were done. The results showed that:The process was the inoculation proportion of Candida tropicalis, Pseudo monas and Candida utilis as 1:2:2, inoculation 2%, 2.0% urea added in Jowar juice, pH 6.0, fermenting 72h at 30℃. The content of fulvic acid could reach more than 20%.5% mixture of Azotobacter chooroccum, Phospho bacteria and Silicate bacteria (2:1:2) were added to the product. The total number of bacteria reached 10 /ml.

研究结果表明：用热带假丝酵母、假单胞杆菌和产朊假丝酵母组合进行液体发酵，接种比例1∶2∶2，接种量2%，在甜高粱秸秆汁中加入2.0%的尿素，pH值为6.0，发酵温度为30℃，发酵72h，发酵产物中生化黄腐酸含量可达20%以上；将圆褐固氮菌、磷细菌、钾细菌以2∶1∶2的比例，按5%加入到发酵产物中，三种细菌总的活菌数可达10~8个/ml；生化黄腐酸复合生物液肥对各种作物的产量均有不同程度的提高，经济效益显著。

At the same concentrations of saccharose , the lactic acid accumulation amounts of the treatments with inoculation are lower than those of the controls , and in controls methylamine is detected because of the decomposition of protein by pathogenic bacteria , but in treatments of inoculation they are not detected .

相同蔗糖浓度下，接种处理的丁酸积累量均低于对照，不接种对照处理中均检测到了腐败菌分解蛋白质产生的甲胺，而接种的则没有。

Mycelium bourgeoning test of solid culturing with liquid strain showed the optimum culture condition was as follows: 4d strain age, 10% inoculation quantity, stirring inoculation mode. Mycelia would overgrow 250ml culturing bottle within 1 week. Compared with traditional technique, the result showed that solid culturing with liquid strain by this means would reduce culture period greatly.

通过液体菌种固体栽培菌丝萌发试验，确定最佳液体菌种种龄为：摇瓶菌种6d，发酵罐菌种4d，接种量为10％，搅拌式接种，以这种方式进行固体栽培，1周内即可使菌丝于250ml培养瓶种发透，与传统方法比较，大大缩短了栽培周期。

The results showed that the population administered with free adjuvant cholera vaccines,both low dose group and common dose group,occurred the weak adverse reaction with 10￣25% rate.The reaction rates between two dose groups of the same vaccine have not significant difference;The reaction rate of the first inoculation(19￣25%) is higher than those of the second inoculation(10￣15%)(P 05);The inoculating local reaction is very light ,only the very few subject occurred the scleroma with 15 mm of diameter.The reaction of aluminum absorbed cholera vaccine is slightly stronger than those of other groups.

结果表明，不加铝佐剂的O139型霍乱菌苗及O1群霍乱菌苗，不论是低剂量组（两剂全程接种45亿菌体）抑或常剂量组（全程接种90亿）接种人群均可产生10～25％的体温呈轻度升高的弱反应，并且同种菌苗的两剂量组的反应率无显著差异；第一剂接种的反应率（19～25％）较第二剂接种的（10～15％）高，P＜0.05；接种局部反应轻微，只有个别受试者出现直径小于15mm的硬结；加佐剂的O1群吸附霍乱菌苗的反应较其它组强。

Microscopic observation showed that the branch was fewer and the space is longer, but the difference of hypha diameter was not distinct. From the invading process, it had a spot of germinated cystospore 24 hours post-inoculation and there was no sporangium 96 post-inoculation when the concentration was 100μg/mL.

从显微镜下观察可见，药剂处理的菌丝分枝相对较少，分枝间距拉长，但对菌丝直径没有影响；从番茄晚疫病菌对番茄叶片的侵染过程来看，浓度为100μg／mL时，24h后，只有少量休止孢萌发，96h后，未产生孢子囊。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。