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After inoculation of 48-72 hours, monolayer was found at the bottom of the plate.

接种后48~72 h 时， 6孔板底部细胞铺满单层。

The aim of the study is to understand the effect of Tobacco etch virus on maize dwarf mosaic disease. In the net house, an experiment is carried out by means of artificial inoculation.

为了研究烟草蚀纹病毒(Tobacco etch virus， TEV)对玉米矮花叶病的预防效果，在防虫网室内，采用人工摩擦接种病毒的方法进行生物学试验，并用RT-PCR和实时荧光定量PCR技术对预防效果进行了检测。

The results indicate that the best conditions of bean residue fermentations should be: adopting Mucor, 70% water capacity of the bean residue, pH6, 10% inoculation amount, about 28℃ in the temperature and 3 days of incubation time.

实验结果表明，豆渣发酵的优化条件为：采用毛霉在豆渣含水量为70%、pH值为6、接种量为10%、温度为28℃条件下发酵培养3d。

After the initial inoculation of measles vaccine and the effects of multiple cropping well, but with age, measles IgG antibody level decreased gradually, to deal with high-grade primary school (grades 4 to 6), junior high, high school students to carry out measles vaccination and immunization do good response, so the prevention and control of epidemics and outbreaks of measles.

经过麻疹疫苗初次接种与复种后效果良好，但随着年龄的增长，麻疹IgG抗体水平逐渐下降，应对小学高年级（4~6年级）、初中生、高中生进行麻疹疫苗强化免疫以及做好应急工作，从而预防和控制麻疹的流行和暴发。

In order to develop a safe and effective immunoadjuvant to enhance the immunity and resistance of animals against infection, a novel CpG Oligodeoxynucleotides containing 11 CpG motifs was synthesized and inserted into the VR1012 plasmid, designated as VR1C. Then the recombinant VR1C was entrapped with Chitosan nanoparticles prepared by the method of ionic cross linkage, and employed to inject muscularly 3-weeks old Kunming mice; the blank VR1012 packed with CNP and saline were used to inoculate mice as the control groups. 28 days after inoculation, all mice were orally fed with 0.4ml 2x108CFU/ per mouse virulent hemorrhagic enteritis E. coli to challenge the resistance against infection.

为研制安全高效免疫调节剂增强动物免疫抗病能力，本实验设计合成含11个C pG基序的寡聚核苷酸，重组构建含CpG的VR1012质粒(VR1C)；制备壳聚糖纳米颗粒包裹重组质粒(CNP-VR1C)，肌注接种3周龄昆明小白鼠，设壳聚糖包裹空质粒和生理盐水对照组；接种后28天口服大肠杆菌攻毒观察小鼠天然免疫的变化和对强毒感染的抵抗力，Sandwich ELISA测定血清免疫球蛋白和白细胞介素含量。

Distribution of silkworm Bm N cell attachment to Cytodex 3 microcarrier was observed and the influence of cell inoculation concentration and microcarrier concentration on cell growth was studied.

选择微载体Cytodex3对家蚕BmN（从SilkwormBombyxmori获得的细胞系）细胞进行高密度培养，并获得成功。

Test teaching results of many years proved that comprehensive application,such as field collection,marcket purchase,natural cultivation,simple inoculation and other materials for preparing slides and materials collection methods as well as temporary preservation,dry preservation at room temperature,cryodrying preservation and cryopreservation,could meet the need of slide making of fungus,bacterium,virus,nemathelminth and other typical disease in a long term.

多年的实验教学证明，综合应用田间采集、市场购买、自然培养、简易接种等制片材料采集方法与临时保存、室温干燥保存、低温干燥保存、冷冻保存等保存技术，可以长期满足真菌性病害各亚门、细菌性、病毒性及线虫性等典型病害制片材料的需要。

Results: The natural infection rate of chidren and women of child-bearing age is respectively 5.19%and 75.13%.There is no bad reaction after inoculating the nettle rash vaccine.All Children group antibody transfer to positive, while women of child-bearing age has only one negative, and GMT increase, it is a positive antibody level to propose long notably after vaccine inoculation.

结果儿童和育龄妇女的自然感染率分别为5.19%和75.13%，接种风疹疫苗后无不良反应，儿童组抗体全部转为阳性，育龄妇女仅1例为阴性，几何平均滴度增高，接种疫苗后显著提升抗体水平。

Unilateral peripheral inoculation of recombinant ectors on the ibrissal pad territory resulted in an about ninefold increase in proenkephalin A mRNA leels in trigeminal ganglion ipsilateral to the infected side.

重组体载体在单侧末梢初级传入去极化范围内的培养，最终导致感染同侧的三叉神经节脑啡肽前体A 的mRNA水平增加约9倍。

Experiment of the third part use the first part"s production as carrier, selecting Geotrich candidum and Candida tropicalis of higher production of protein also selecting producing the cellulose enzyme Bacillus subtilis FS and Lactobacillus bulgaricus by ourselves separated, design orthogonal test and a series of single factor experiment, finally assurancing the inoculation quantity of four strains germ, is Geotrich candidum 5%, Candida tropicalis 5%,Bacillus subtilis FS 5% of FS, Lactobacillus bulgaricus 3%, the most suitable development temperature is 30 "C , the development time is 15 days.

实验的第三部分是以第一步共培养所得的培养物为载体，选择产蛋白量高的白地霉、热带假丝酵母及本室筛选的产纤维素酶芽孢杆菌FS和自行分离的保加利亚乳酸杆菌，设计正交实验和一系列单因素实验，最终确定了四株菌的接种量，分别为白地霉5%，热带假丝酵母5%，FS5%，保加利亚乳杆菌3%，最适培养温度为30℃，培养时间为15天。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。