查询词典 inoculating

And the conditions in cell suspension culture of Mikania micrantha were studied. The results showed that sucrose was the compatible carbon sucrose, and 30g/L sucrose concentration can satisfy the growth of Mikania micrantha cell; ammonium was absorbed under different sucrose concentration that haven't demonstrated significant specificity, and was completely absorbed on the lag phase and the early logarithmic phase; while nitrate was mainly absorbed on logarithmic phase. The density-dependent of Mikania micrantha cell starting to grow and density-inhibited of cell growth were proposed, the fittest inoculating quantity of Mikania micrantha in cell suspension culture was 40g/L.

并对微甘菊细胞悬浮培养条件进行研究，结果表明，微甘菊细胞生长适宜的碳源是蔗糖，并且较合适的蔗糖浓度为30g／L；在不同蔗糖浓度下氨基氮的吸收差别不大，氨基氮在迟滞期和对数期前期已基本消耗完毕，对数期生长主要利用硝基氮，因而提出，在微甘菊细胞液体悬浮培养中，氮源可以采用初始低氨基氮浓度，对数期中间维持高硝基氮，而且对数期中逐步流加少量的氨基氮；微甘菊细胞迟滞期分裂启动存在密度依赖现象，对数期生长存在密度抑制现象，最适接种量为40g／L。

Results: The TRAP positive multinuclear cells were induced from rat marrow mononuclear cells after inoculating for 5 clays. RT-PCR analysis showed that ASIC1, ASIC2 and ASIC3 mRNA were present in the osteoclasts, and the corresponding proteins expressions were detected.

结果：诱导5d后可见抗酒石酸盐酸性磷酸酶阳性多核细胞出现，大鼠破骨细胞在mRNA和蛋白水平均可检测到ASIC1、ASIC2和ASIC3基因的表达产物。

And 6. 0, suitable dissolved oxygen and certain quantity of inoculating size would be helpful to the production of phytase.

在较优的培养基和适宜发酵条件下，A.niger496-1菌株的最高酶活力达到了297u／mL。

The resistance to disease of transgenic plants were tested by inoculating leaves with Puccinia coronata var.coronata cummins.

对所得到的转基因草地早熟禾植株人工接种冠锈菌夏孢子进行抗病性鉴定并测定了抗病性相关指标。

Two-dimensional gel electrophoresis were employed to analyze the proteome from leaves of cucumber R17 at 24, 48 and 72 h, after inoculating Sphaerotheca fuliginea .

以黄瓜抗白粉病品系R17为试材，在接种白粉病菌24、48和72 h后提取叶片蛋白质，采用双向电泳技术研究白粉病菌胁迫条件下黄瓜叶片蛋白质组的变化。

The results indicated that effectiveness among the strains and the alfalfa cultivars was great different. Compare with the control uninoculated treatment, inoculating has significantly increased alfalfa dry matter and nodule numbers.

不同苜蓿品种接种不同菌种，相互间差异较大，与中苜一号匹配较好的菌系是NM006、30085和45236，；与RS匹配较好的菌系是30015、QH180和45236；与Vector匹配较好的菌系有45236、30015和30085；与WL323匹配较好的菌系是30015、45236和25132。

The results show that compared with the uninoculated pear fruit, the PPO activity of the pear fruit inoculated by the pathogen markedly increase in 48 hours after the inoculating treatment, the PAL activity is on the rise during the 120 hours after inoculation, POD and CAT activities reach a peak at 72h after inoculation, and the SOD activity of the inoculated treatment is also obviously higher than that of the contrast, which suggests that those enzymes are closely-related to pear fruit against Botryosphaeria berengriana f.sp.

结果表明：鸭梨果实中多酚氧化酶活性在接种轮纹病菌48 h内显著高于对照，苯丙氨酸解胺酶活性在接种120 h内一直呈上升趋势，过氧化物酶和过氧化氢酶活性在接种后72 h出现活性高峰，超氧化物歧化酶活性在接种后120 h内也显著高于对照，说明这些酶对鸭梨果实抵抗轮纹病密切相关。

When inoculating, the modalities ofcells maybe round, triangle or virgulate; and began to be adhesive for 48h; andformation of colony could be formed on day 4-5; Many cells grew in clonalmanner on day 7, and the cultured cells were characterized by large spindle-shapedappearance by the time of 10 days.

刚接种时细胞形态呈圆形、三角形或棒杆状，48h 后细胞开始贴壁，4-5d 可见有集落形成，第7d 形成多个细胞克隆，第10d 细胞呈长梭形，2w 左右可达到基本融合；传代细胞呈均匀的纺锤形，基本上无悬浮细胞。

Inoculating test plants through vector,observing symptom and using universal primers(R16mF2/R16mR1)for phytoplasma 16S rDNA were conducted.1.4 kb DNA fragments were amplified by polymerase chain reactionin DNA samples extracted from inoculated wheat and viruliferous Psammotettix striatus L.respectively.

采用介体接种植物，症状观察和应用植原体16S rDNA基因通用引物对R16mF2/R16mR1进行PCR扩增，在接种小麦和传毒介体中均扩增出1.4kb的特异片段，鉴定出小麦蓝矮植原体新寄主7种。

The production and characterization of endoglucanase from Streptomyces xylophagus KX6 was studied. Maximum endoglucanase yield of 0.538 IU/ml was achieved with medium pH8.0, containing CMC2Na 1.0% as carbon resource, soybean meal 1% as nitrogen resource, 2% inoculating volume, 30% 250 ml triangle flask bulk for medium volume at 40℃ 200r/min shaker for 48h.

实验中对其产酶的液态发酵条件进行了研究，碳源为1%羧甲基纤维素钠，氮源为1%豆粕粉，250ml三角瓶30 %装液量，接种量为2%，培养基初始pH为8.0，培养温度为40℃，200r/min培养48h后，发酵液中内切葡聚糖酶活达到0.538IU/ml。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。