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In vivo tumor metastasis assay, three group cells were inoculated into footpad of 615 mouse subcutaneously. After three weeks, the mice were killed and their lymph nodes were isolated.

结果：1.Gelsolin在小鼠腹水型肝癌细胞系Hca-F细胞和Hca-P细胞胞浆中表达，Western-blot分析显示gelsolin在Hca-F细胞中高表达，在Hca-P细胞中表达较低(P.05)。2。

Furfur was inoculated to the mycelial culture liquid medium and the rapeseed oil culture liquid medium, and then incubated in vibrator to induce the production of mycelial phase and yeast phase of M.

用全脂牛奶平板法、Folin-酚法、QuantiCleave蛋白酶试剂盒测定菌丝相及酵母相糠秕马拉色菌胞外蛋白酶的活力，并检测温度、pH值对蛋白酶活性的影响。

These cells were then cultured in RPMI 1640 with 0.3% gelose for 10 to 14 days and the colonies were counted. LoVo cells and A549 cells were also inoculated subcutaneous in BALB/c nude mice.

将裸鼠皮下接种LoVo，A549细胞，72h后分组，每次注射20万IU/kg或100万IU/kg，500万IU/kg IFN-β，每天1次，连续10d，同时设置生理盐水对照组、标准品组和注射用环磷酰胺治疗组。

The half-life day of HCH, DDT and chlorpyrifos was 27～32, 27～33 and 22～24 respectively and the residue were detected in the substrates and fruitbodies when fungi were inoculated to the substrated, There were no cypermethrin detected in the fruitbodies, and it's half-life day in the substrate was 19～20. Carbendazim used for control germina was also detected in the substrates and fruitbodies with a half-life from 19 days to 20 days. Because of it's short half-life (2 days to 4 days), the degradation of Chlorothalonil in substrates and fruitbodie were quickly and no chlorothanonil were detected both in the substrates and fruitbodies when harves.

在接菌情况下，HCH、DDT和毒死蜱等杀虫剂在食用菌子实体和培养料中都有残留，培养料中的降解半衰期分别为27～32天、27～33天和22～24天；氯氰菊酯在培养料中的降解半衰期为19～20天，但在子实体中没有残留；多菌灵是防治食用菌病害的常用杀菌剂，在培养料和子实体中也都有残留，并且培养料中的降解半衰期为19～20天；百菌清极易降解，在培养料和子实体都没有残留，在培养料中的降解半衰期仅为2～4天。

Core in sterilized soil was inoculated with two strains of Glomus mosseae and G. caledonium.

用两种不同的VA菌根真菌Glomus nosseae和Glomus caledonium接种玉米进行盆栽试验。

Experiments were carried out to study the effects of P sources on phosphatase activity of rhizosphere and mycorrhizosphere of red clover inoculated with Glomus mosseae , and cultural system with three compartments was applied.

以红三叶草为研究对象，利用三室培养系统，在接种菌根真菌的条件下研究了不同磷源对根际和菌根际磷酸酶活性的影响。

objective to investigate the inhibitive effect of pseudolaric acid a on sarcoma 180(s180) cells inoculated into the hypoderm of mouse armpit.

目的 探讨土槿甲酸对接种于小鼠腋部皮下的肉瘤180（s180）细胞的抑制作用。

s180 cells were inoculated into the hypoderm of mouse armpit. different concentration of paa was then given to these mice for seven days by way of peritoneal cavity injection.

在小鼠腋部皮下接种s180细胞，随后用不同浓度的paa给各组小鼠作腹腔注射7 d。

ABSTRACT:OBJECTIVETo establish neoplasm transplantation models of melanoma in BALB/c miceMETHODSB16 melanoma cells in logarithmic growth phase were inoculated into BALB/c mice's back hypodermis and feet with different concentration to establish neoplasm transplantation of melanoma.

［目的］建立宜于进行早期观察及测量的小鼠黑色素瘤模型［方法］将不同数量的处于对数生长期的B16细胞株黑色素瘤细胞接种于BALB/c小鼠背部及足垫皮下，建立移植肿瘤动物模型。

Recombinant bovine prion protein was treated by chemical solution, and the treated Recombinant bovine prion protein was intracerebrally inoculated in the brain. The prion gene expression was quantified and the proteinase resistant protein was detected by western-blotting.

对重组牛朊蛋白进行了化学处理后脑内接种金黄地鼠，200天后对朊蛋白抗性进行了western-blotting检测，对脑组织朊蛋白基因的表达进行了分析。

I can not make it blossom and suits me

我不能让树为我开花

When temperatures are above approximately 80 °C discolouration of the raceways or rolling elements is a frequent feature.

当温度高于 80 °C 左右时，滚道或滚动元件褪色是很常见的特征。