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Following 72 h co-culture, desquamate, sporozoites, trophozoites, meronts, microgametocytes, macrogametocytes, zygote, thin-wall oocyst, and thick-wall oocyst appeared orderly. Between the 60th and 72th hour, many oocysts emerged. Inoculated by the C. parvum -infected cell culture supernatant at the 48th hour, the immunosuppressed mice became infected.

在感染后72 h内，隐孢子虫出现连续发育阶段，包括脱囊、子孢子、裂殖子、裂殖体、滋养体、配子体、合子、薄壁卵囊和厚壁卵囊，在60～72 h内形成卵囊；用感染48 h的细胞培养上清接种于免疫抑制小鼠， 10 d后有隐孢子虫卵囊排出。

Every explant was pricked with a dissector at the center of one subregion before inoculated on MS+BA 1mg/L+NAA 4mg/L+2,4D 05mg/L medium and incubated in darkness for 7 days,then transfered to MS+BA 1mg/L medium.85% of the explants regenerated somatic embryos directly around the prick after 40 days.

每一叶片在1个亚区中心部位用解剖针刺伤1点后接种于MS＋BA1mg／L＋NAA4mg／L＋2，4D05mg／L＋蔗糖20g／L的培养基上，暗培养7d后，转接到MS＋BA1mg／L＋蔗糖20g／L的培养基上继续暗培养，40d后，85％的叶片在刺伤口周围发生直接类型体细胞胚胎。

Study on formation of Dragon's blood in Dracaena cochinchinensis inoculated with Fusarium

特异性真菌作用于龙血树材质形成血竭的研究

The apical buds and axillary buds of Dracaena cambodiana Pierre ex Gagnep. were inoculated on MS medium containing 1 mg/L BA, 0.1 mg/L NAA, 100 mg/L PVP and 30 g/L sucrose for inducing shoots. After cultured for 40~50 d, the shoots bourgeoned. They were then transferred onto MS medium supplemented with 2 mg/L BA, 0.5 mg/L KT and 30 g/L sucrose for inducing clustered buds. After 25~10 d, the clustered buds formed. The proliferation rate of the clustered buds was 300%~500% per month.

以海南龙血树的顶芽和侧芽作为外植体，把其接种于MS+BA 1 mg/L+NAA0.1 mg/L+PVP100 mg/L+蔗糖30 g/L培养基上培养40~50 d可诱导其腋芽萌发，再把萌发后所形成的新芽切割下来接种于MS+BA 2mg/L+KT 0.5 mg/L+蔗糖30 g/L的培养基上培养25~30 d可诱导形成丛生芽，丛生芽在继代培养过程中每25~30 d可增殖3~5倍。

After 45 days of culture, the production ratio reached 1. 21g/g dry inoculated cell weight, while that in shaking flask after 72 days was

在该操作条件下对紫草细胞进行了双液相悬浮培养试验，热模试验的结果证明了反应器能满足细胞的培养要求。

After using both of the two immunosupprssion methods, we can establish the animal model successfully when inoculated by CSO, and the efficacity of the second method is better than the first one.2 In order to further understood the endogenous development of Cryptosporidium human strain in mice.

作者采用19日龄昆明系小白鼠作为模型动物，随机分成8组，分3个感染剂量进行人源隐孢子虫分离株感染系列筛选试验，并确定随剂量和时间不同对人源隐孢子虫在小白鼠寄生部位的影响。

The effect of its being inoculated with anaerobic sludge on the electrogenesis capacity of glucose simulated wastewater has been investigated.

另外，还对以水及好氧污泥作为电池阴极时系统的产电性能进行了对比。

A set of spelt monosomic lines as female parent were respectively crossed with wheat new variety SM96 and new line SM20005 to determine the chromosomal location of the gene conferring resistance to powdery mildew in both. Seeding of parents, F〓 and F〓 from monosomic F〓 were inoculated with race no. 15 of Blumeria graminis f. sp. tritici. Analysis of obtained data revealed that one major dominant resistant gene originating from wild Emmer in the variety SM96 and the line SM20005 was located on chromosome 4A.

为确定小麦新品种沈免96和新品系沈免20005含有的抗白粉病基因所在染色体，以一套斯卑尔脱单体系为母本分别与沈免96和沈免20005杂交，将亲本、F〓、单体F〓植株的F〓代群体在苗期用小麦白粉菌15号小种的单孢菌系接种鉴定，单体分析结果表明，沈免96及沈免20005所含源于野生二粒小麦的抗小麦白粉菌15号小种的1个显性主效基因位于4A染色体上。

Inoculating lactic acid bacteria resulted in a rapid drop in pH,and had the ability to control the growth of pathogenetic microorganisms in ensilage of the corn straw. The fiber content of the straw inoculated with cellulase as decreased from 35.3% to 25.3%,and the level of water soluble carbohydrate was higher than that of the control.

玉米秸秆黄贮过程中接种乳酸菌可以迅速降低原料的pH值，有效抑制腐败菌生长；添加纤维素酶，可以使秸秆的纤维含量由原来的 35 。3%降低到 2 5 。3%，并增加可溶性碳水化合物含量；同时使用酵母菌，可使粗蛋白含量提高 10 %。

The region between the circular brown band generated by cell lipid exosmosis and the band of the wound in the non-transgenic cotton Z35's leaves, is wider than that of the transgenic cotton T-34's leaves, and no micro-HR was found in Z35, the intensity of leaves wilting is much serious, by contrast, micro BR was found around the wound region within the transgenic cotton leaves inoculated with the pathogen, but the micro-HR was not found in the transgenic cotton treated with water and around wound region of non-transgenic cotton Z35 leaves which showed that there was micro-HR defence in transgenic hpa1xoo cotton.

棉花黄萎病菌侵染叶片后，非转基因棉花叶部伤口周围细胞无微过敏性反应，受病原菌侵染的叶片萎蔫程度较重；转hpa1xoo基因棉花叶部伤口周围细胞有微过敏反应，叶部刺伤处仅有较小的坏死斑，且不相连，发病较轻，而清水处理的转基因棉花与非转基因棉花叶片中伤口周围均无微过敏反应，说明病原菌侵染诱导转hpa1xoo基因棉花产生微过敏防御反应，转hpa1xoo基因棉花较非转基因棉花有较强的抗病性。

I can not make it blossom and suits me

我不能让树为我开花

When temperatures are above approximately 80 °C discolouration of the raceways or rolling elements is a frequent feature.

当温度高于 80 °C 左右时，滚道或滚动元件褪色是很常见的特征。