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Methods: Thirty extracted human molars whose pits and fissures on the occlussal surface had been inoculated with Escherichia coli were randomly divided into three groups: group A was treated with newly confected phenol-formaldehyde resin which resinified the dental fissure contents, group B was sealed Bis-GMA resin in pits and fissures and group C as the control group was only treated by cleaning.

选择30颗人离体磨牙，在咬合面点隙裂沟接种大肠杆菌，随机分为3组；A组以新配制的酚醛树脂使点隙裂沟内容物树脂化，B组以成品Bis-GMA封闭剂作点隙裂沟封闭，C组仅进行表面清洗作为对照组；各组分别沿裂沟点隙处片切牙齿，放入LB培养基培养，观察大肠肝菌生长情况。

Results:We hae screened out the best method of drawing the materials from OB,which is crushing directly. we also conside the immunoadsorption as the best method of puring OECS.Coating the petri dish is profitable to pure the cells. meanwhile the appropriate cells density inoculated is important for eentual purity of culture.

结果：我们从中筛选出最佳取材方法为直接挤压法，最好的纯化嗅鞘细胞方法为免疫吸附法，同时发现包被培养皿对纯化细胞意义重大，而高密度接种细胞对提高细胞的最终纯度也是非常有利。

Due to potential instability of the prophages carrying the stx gene, in this study, the prophage of O157:H7 86-24 strain was cured with serial passages of bacteria, confirmed by PCR and DNA sequencing. The cultural supernatant of O157 prophage curing mutant was inoculated into Vero cells, indicating that the prophage curing mutant of O157 lost the toxigenicity to Vero cells.

由于原噬菌体本身具有潜在的不稳定性，本研究以O157：H7 86-24为始发菌株，将其进行传代培养，筛选原噬菌体消除菌株，对原噬菌体在O157基因组中的整合位点进行分析，并用Vero细胞对原噬菌体消除菌株的细胞毒性进行鉴定。

In order to explore the effects of PCV2 infection on PAM functions at the cellular, molecular, and genie level, PAMs from forty-day-old healthy piglets inoculated oro-nasally with PCV2 BF strain were collected at different days ...

通过测定感染后PAM的凋亡与吞噬功能、表面分子与抗原递呈功能相关分子及细胞因子的表达动态，从细胞、分子与基因水平系统分析了PCV2感染对猪肺泡巨噬细胞功能的影响。

The artificial infested soil containing 30 oospores per gram of air-dry soil was saturated with water and smeared on the basal stalk (0~10 cm) of the tested plants. The inoculated site was wrapped with a piece of aluminum foil to keep the inoculum from drying out.

接种时，以每克土约含30个卵孢子之壤土加水达於饱和含水量后，涂抹在供试玉米植株的茎基部(0~10cm)，并以铝箔包裹之，以防接种病土乾燥。

Methods Live attenuated rubella vaccine virus strain RA27/3 was inoculated to the monolayer of human diploid MRC-5 cells at an appropriate MOI and incubated in serum-and protein-free CDM culture medium. The virus liquid was harvested and, after addition of polyvinylpyrrolidone as a stabilizer and protective agent, lyophilized. Perform overall control tests on the prepared vaccine.

将风疹减毒活疫苗毒种RA27/3株按适当比例接种单层人二倍体细胞MRC-5株，使用无血清、无蛋白的CDM培养基培养病毒，收获病毒液，加入聚乙烯吡咯烷酮作为病毒稳定剂，制备疫苗原液，加入冻干保护剂，制备5批冻干疫苗，进行各项检定。

Methods Live at-tenuated rubella vaccine virus strain RA27 /3 was inoculated to the monolayer of human diploid MRC-5 cells at an appropriate MOI and incubated in serum-and protein-free CDM culture medium.

方法将风疹减毒活疫苗毒种RA27/3株按适当比例接种单层人二倍体细胞MRC-5株，使用无血清、无蛋白的CDM培养基培养病毒，收获病毒液，加入聚乙烯吡咯烷酮作为病毒稳定剂，制备疫苗原液，加入冻干保护剂，制备5批冻干疫苗，进行各项检定。

Leaf curling symptoms could be induced in Nicotians benthamiana and N. glutinosa plants when PaLCCNV were co-inoculated with ToLCCNV DNA β.

将PaLCCNV、ToLCCNV和ToLCCNVDNAβ三者共同接种后，PaLCCNV和ToLCCNV都能在寄主植物上复制，并且诱导曲叶症状。

It took place not only beside the inoculated dot, but also in the position where the position nematodes never arrived.

它不仅发生在接种点附近，而且发生在线虫还没有移动到的远离接种点的部位。

Methods:Specimens were inoculated on sabouraud culture medium and that on CHROMagar Candida at the same time, then the yeast fungus which grown on sabouraud culture medium was identified by API20C AUX, while that on CHROMagar Candida was judged directly according to the color and appearance of colonies .

将标本同时接种沙氏基和CHROMagar念珠菌显色培养基，沙氏基上生长的酵母菌经API20C AUX酵母菌鉴定系统鉴定，CHROMagar念珠菌显色培养基根据菌落颜色、形态直接判读。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。