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Increasing of the proliferation and differentiation of lymphocytes in LP were found. Conclusions Lymphocyts in LP play an important role on the mucosal immunity of SPF mice when they are first inoculated with Candidas. The reducing amount of Candidas in intestine is related to increasing of the specific IgA antibodies in the intestinal mucus.

带菌动物首次白念菌肠内给入后以固有层中淋巴细胞反应为主，增殖及分化均增加；白念菌总量和粘附数量减少与局部粘膜免疫反应及特异IgA抗体含量上升的关系十分密切。

Methods After Candida albicans were inoculated into the specific pathogen free mice, the total amount of Candidas in cecum and the amount of which attached to the mucosal membrane were counted at different interent intervals. The lymphocyte proliferation in Peyer's patch and in lamina proper was shown by BrdU incorporation, meanwhile B cells isotype switch in PP was investigated. IgA plasma cells were shown by immunohistochemical staining. Specific IgA antibodies to Candida albicans were measured with ELISA.

采用无特殊病原菌动物经口喂入白念菌，在不同时相处死后，观察肠内白念菌总数及肠粘膜表面白念菌粘附数量；取肠系膜淋巴结做白念菌选择培养，观察移位体内发生率；采用Brdu体内掺入显示局部粘膜淋巴细胞的增殖情况；流式细胞计数潘伊尔氏结细胞表面IgA阳性率；免疫组化染色后计数固有层中IgA浆细胞数量变化；ELISA法测定肠粘液中特异抗白念菌IgA含量。

Glucose was fermented in a chemostat under anaerobic condition inoculated by mixture of two kinds of sludges and rumen from sheep.

以两种厌氧污泥和一种绵羊胃液的混合物为接种物，对葡萄糖进行厌氧发酵。

In an earlier investigation [14], chemostats were inoculated with surface water from the same location as in the present study and grown for 40 days on a rich medium containing a mixture of vitamins and 3 mg/l of the fungicide 2,6-dichloro-4-nitroaniline.

在一次更加早期的调查[ 14 ]， chemostats 被接种了与水面从地点和一样在本研究中和增长40 天在一个富有的媒介包含混合物维生素和3 毫克杀真菌剂2，6 dichloro 4 硝基苯胺(dichloran 的) l 。

A kind of polymer degrading microbe JHW-31 was selected from Daqing Oilfield. The microbe was inoculated and cultured in the culture medium confected with polymer and a little sucrose for 7 days under the condition of pH 7.2 and temperature 45℃. The viscosity reduction rate of the polymer reached 92.1%,and the molecular weight of the polymer decreased from 18×106 to 0.5×106. The gross of the polymer decreased a little bit.

摘要从大庆油田筛选到聚合物降解菌种JHW-31，将菌种置于45℃、pH值为7.2的环境条件下，在添加少量蔗糖的聚合物培养基中培养7d，聚合物黏度可降低92.1%，并使其分子量从18×106降至0.5×106，聚合物总量也有所减少。

Op-HA/PLGA and PLGA chloroform solution were covered on the surface of coverslip of which rabbit osteoblasts were inoculated and cultured in vitro for 7 days.

将op-HA/PLGA和PLGA氯仿溶液分别于盖玻片表面铺成薄膜，并接种兔成骨细胞，体外培养7 d。

The primers and the detection programs have been used to detect the pathogens in the inoculated-diseased cucumber plants .The details are reported as follows: 1. By using the primers ITS1/ITS4, only Fusarium isolates could be amplified to produce a special fragment of 750bp. This product generated 480bp, 200bp and 100bp fragments after digested by restriction enzyme HinfⅠonly from the pathogen of Cucurbits Fusarium Wilt.

引物ITS1/ITS4只在镰孢属菌株上扩增到一条750bp分子片段，其他供试菌株无扩增条带产生，扩增产物经HinfⅠ酶解后，只有瓜类尖镰孢菌产生480bp、200bp和100bp 3条带，说明该PCR-RFLP程序对瓜类尖镰孢菌具有特异性；巢式PCR引物BIK1/BIK4和BIK2/BIK3只有在黄瓜尖镰孢菌菌株上扩增到一条800bp分子片段，其他菌株均无扩增条带产生，说明该巢式PCR程序对黄瓜尖镰孢菌具有特异性。

Glutinosa and Solarium lycopersicum,while Tomato yellow leaf curl China virus and its DNAβinduces distinctive vein thickening and enations as well as leaf curling.To investigate the genetic determinants of the phenotypic differences, we inoculated Nicotiana spp.with infectious clones of TbCSV/DNAβand TYLCCNV/DNAβpseudorecombinants and showed that symptoms co-segregated with the DNAβcomponent.

烟草曲茎病毒(Tobacco curly shoot virus，TbCSV)/DNAβ在烟草和番茄上能引起曲叶症状，而中国番茄黄化曲叶病毒(Tomato yellow leaf curl China virus，TYLCCNV)/DNAβ除引起曲叶外还引起寄主的脉突和耳突症状。

Objectives To investigate the mechanism of generation of L-form of M.tuberculosis and its significance on the development,diagnosis and treatment of tuberculosis. Methods M. tuberculosis was inoculated into the non-high osmotic medium with rifampin, isoniazid or ethambutol and then the L-form was observed by microscopy daily.

目的 观察常用抗结核药物对结核分支杆菌L型的诱导作用以及结核分支杆菌L型在非高渗透压培养基内的某些特性及其抗菌药物敏感性，探讨结核分支杆菌L型形成的机制及其在结核病的发生、诊断及治疗上的意义。

0.5-1.0 cm segments of single leaf of Wanshu 7 were pruned and inoculated on MS solid media with different hormone, and cultured for 30 days in the fallowing conditions of temperature of (27±1)℃, photoperiod of 16 h/d and light intensity of 1 500-2 000 lx.

方法］剪取万薯7号0.5~1.0 cm长的单叶节段，接种到不同激素处理的MS固体培养基，在温度(27±1)℃，光周期16 h/d，光强1 500~2 000 lx条件下培养30 d。

Do you know, i need you to come back

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Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。