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Methods 2-3 days old inbred Balb/C suckling mice were inoculated intracerebrally with tissue suspension of soft parate petechia of HFRS patients and the HFRS virus RNA was tested in brain, lung and kidney tissues of sucking mice by nested PCR.

2～3天的近交系Balb/C乳鼠，脑内接种早期HFRS重症患者软腭粘膜出血点组织悬液，用套式聚合酶链式反应检测乳鼠脑、肺及肾组织中的HFRS病毒RNA。结果接种乳鼠发病死亡，剖检显示

In the early stage, the pathological change degree of parenchymatous cells upwards inoculated dot were more gently than those downwards. Then they went to be consistent later.

相同接种时间内，与接种点上下距离相同的松苗茎段细胞病变的趋势与线虫上下移动快慢差异是一致的。

In the same time, the pathological changes degree of parenchymatous cells which far away inoculated dot were more gently than those were near. Parenchyma axial died earlier than parenchyma radial.

接种相同时间，距离接种点较远部位的薄壁细胞相对较近部位的病变程度轻、数量少。

In group A, VX2 tumor tissue was inoculated into the rabbit kidney percutaneously under ultrasound guidance.

新西兰大白兔30只平均分为两组，A组采用超声引导下组织块注射法，B组采用手术直视下组织块接种法建立VX2肾癌模型，比较两种方法的成瘤率，种植后隔天一次监测VX2肾癌的超声声像变化。

The activities of PODSOD or CAT and H2O2 content were examined in cucumber leaves treated with BTH or inoculated with Pseudo peronospora cubensis f. sp. cucumerinum.

以'长春密刺'黄瓜幼苗为材料，对苯并噻二唑处理或霜霉菌接种后黄瓜叶片的过氧化物酶、超氧化物歧化酶、过氧化氢酶活性与H2O2含量的变化进行了研究。

And then, Plasmodiophora brassicae were inoculated on transformed roots and transformed plants respectively for identification and screening of club root disease-resistant transformants.

根据转化体上根肿病的发病程度，评价不同转化系对根肿病的抗病性表现以及抗病转化系的抗病机理。

In order to ascertain the susceptibility and the potential danger of WSSV to the cultured Portunus tritaberculatus, the healthy crabs were inoculated with WSSV by intramuscular injection and feeding.

利用对虾白斑综合征病毒WSSV，采用注射和投喂方式，对养殖三疣梭子蟹进行人工感染，以确定WSSV对养殖梭子蟹的易感性和可能存在的潜在危害。

In addition,disease incidence and lesion area of muskmelon inoculated with T.roseum could be controlled effectively by preharvest BTH treatment in the period of storing.

此外，采前BTH 4次处理还能明显降低果实采后损伤接种粉红单端孢的发病率和降低病斑面积。

With the protocorms of C. grandiflorium as inoculated materials and MS as basic medium, different combinations of growth regulating substances with different concentrations and different hormone proportions were designed to discuss the effects of different medium on the protocorm propagation, bud induction and rooting of C. grandiflorium and the effects of different hormone matching on the formation of roots.

方法］以大花蕙兰的原球茎为接种材料，以MS为基本培养基，设计不同生长调节物质浓度的组合、不同激素配比，探讨了不同培养基对大花蕙兰原球茎增殖、诱导芽及生根的影响和不同激素配比对形成根的影响。

For the sake of developing an effective, safe and no pollution disinfectant to reduce or eliminate food-borne pathogens on shell eggs, the bactericidal activity of neutral electrolyzed water (NEW, pH 6.0-7.5) generated by electrolysis of diluted hydrochloric acid in a non-membrane electrolytic cell for inactivation of Salmonella pullorum, Escherichia coli O157:H7 and artificial inoculated shell eggs initial logarithm of population of two pathogens on the eggs were approximately 6.19-6.26 log10 (cfu/g and 6.12-6.19 log10 respectively was evaluated.

为寻求一种高效、安全、无污染的禽蛋清洗消毒剂，采用无隔膜电解装置电解稀盐酸溶液制备中性电解水（pH值6.0～7.5）考查不同有效氯浓度、处理时间和温度条件下中性电解水对鸡蛋人工接种鸡白痢沙门氏菌（Salmonella pullorum，鸡蛋表面的初始菌落数对数为6.19～6.26 log10）和大肠杆菌O157：H7（鸡蛋表面的初始菌落数对数为6.12～6.19 log10）的杀灭效果。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。