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The results were as follows: 1.Inoculated on the induction medium at the late uninucleate stage,the microspores could initiate embryogenesis by first divisions in both symmetric and asymmetric patterns,but the former was predominant.

通过观察玉米小孢子胚胎发生的细胞形态学特征，发现：离体培养条件下单核后期的花粉可以通过均等分裂和不均等分裂两条途径形成胚状体，与前人的研究结果一致。

Recombinant plasmid pSVH 7 DNA of avian influenza virus H7 subtype heamagglutinin gene was encapsulated with DC-chol/DOPE liposomes and PC/chol/SA liposomes separately. Two-week old SPF chickens were intramuscularly inoculated with 50 μ g/0.2ml of the liposome entrapped PSVH 7 DNA. Four-weeks later, each chicken was challenged with 0.1ml 〓 AIV . One week after the challenge, the secretion of the cloacas was collected and transfected to chicken embryos to isolate the virus. The virus was isolated from 6/6 of the control group, 1/6 of the naked DNA group, 1/6 of the PC/chol/SA entrapped DNA group and 0/6 of the DC-chol/DOPE liposome entrapped group. The HI antibody titers (log2) of the four groups were 6. 83±0.98, 7. 0±1. 26, 7. 83±1. 17 and 8. 00±0.89 respectively 1-week after challenge, and 8. 5±0.55, 8. 17±0.82, 8. 68±0.45 and 9. 33±0.54 respectively 2-week after challenge. The results showed that inoculation of liposome entrapped DNA significantly enhanced resistance to virosis in animals.

将含禽流感病毒H7亚型血凝素基因的重组质粒pSVH7用DC-chol阳离子脂质体和胆固醇／卵磷脂／十八胺脂质体包裹，免疫2周龄SPF鸡，4周后用同型禽流感病毒进行人工感染，1周后采集泄殖腔分泌物分离病毒，结果未免疫组6／6分离到病毒，裸质粒DNA免疫组1／6分离到病毒胆固醇／卵磷脂／十八胺脂质体包裹DNA免疫组1／6分离到病毒，DC-chol脂质体DNA免疫组没有分离到病毒（0／6）：人工感染后1周各组的HI抗体（Log2）分别为6.38±0.98，7.00±1.26，7.83±1.17，8.00±0.89，2周后为8.50±0.55，8.67±0.82，8.68±0.45，9.33±0.52，脂质体包裹组在同期均高于未免疫组和裸DNA免疫组，表明脂质体包裹质粒DNA免疫动物后，能增加动物对病毒感染的抵抗力和反应能力。

This paper summarized factors, both in biologic and abiological aspects that affected successful colonization and activity of bacterial inoculation. The impacts of inoculated bacteria on population structure of specific rhizobacterial communities and environment should also be paid an eye on.

本文主要针对国内外在影响微生物根际定殖的因素以及影响引入微生物活性的因素两方面的研究进行综述，同时强调对接种剂引入所引起的生物种群及生态环境变化应予以重视。

The results showed that under 8% of raw total solids concentration, 6.8-7.4 of pH, the methane tank sludge, 20% of them inoculated, after 30 days of fermentation, it was 9 days of period that the effect was execellent. The total aerogenesis tolerance was 42490.70 mL, the day aerogenesis tolerance was 1416.36 mL, the pit held aerogenesis rate was 0.217 m~3/m~3·d , the stuff held aerogenesis rate was 0.241 m~3/m~3·d, the raw material aerogenesis rate was 0.090 m~3/kg·TS.

结果表明，在原料的总固体浓度为8％、pH值介于6.8～7.4、接种沼气池污泥量为原料干物质重量的20％、发酵周期为30d时，对发酵系统间隔9d换料的效果最好，其在总产气量、日均产气量、池容产气率、料容产气率和原料产气率分别达到了42490.70mL、1416.36mL、0.217m~3／m~3·d、0.241m~3／m~3·d和0.090m~3／kg·TS，都比不换料的对照处理有很大提高，分别提高了153.41％、153.41％、152.33％、153.68％和150％，沼气中的甲烷含量也在70％以上。

Methods The specimens were inoculated into amniotic and allantoic cavities of the embryonated chicken eggs for viral isolation.

病毒分离采用常规的鸡胚双腔接种，毒株检定用常量半加敏HI测定。

In order to discuss the optimal condition in which GPMV proliferates in chicken embryo and its pathogenicity, GPMV of different titer was inoculated into 911-day chicken embryo and the allantoic fluid was harvested after 36, 48, 60, 72 and 84 h, the HA titer was measured as well.

为了探讨鹅副粘病毒在鸡胚中增殖的最佳条件及其致病性，将不同血凝滴度的鹅副粘病毒分别接种于9~11日龄鸡胚尿囊腔，收获孵化36、48、60、72和84 h的鸡胚尿囊液，测定血凝滴度。

XRD, EDS, ETIR and SEM reveal the precipitate obtained from YN23-inoculated flasks to be a mixture of potassium jarosite and ammoniojarosite, with morphological features similar to the latter.

接种25h后，当Fe(上标 2+)氧化率达到90%时，开始出现沉淀，pH也达到1.92的最高值；到第95h，当沉淀累积到7.9gl^(-1)，时，沉淀反应停止，此时pH达到1.32的最低点。

P. ussuriensis seedlings mixed with Amorpha fruticosa are inoculated by VA mycorrhizal fungi could make P.

大青杨与紫穗槐混交并接种丛枝菌根真菌后，可使大青杨根际营养&富集&。

Ussuriensis seedlings mixed with Amorpha fruticosa are inoculated by VA mycorrhizal fungi could make P. ussuriensis rhizosphere nutrient enrichment.

大青杨与紫穗槐混交并接种丛枝菌根真菌后，可使大青杨根际营养&富集&。

P. ussuriensis seedlings mixed with Amorpha fruticosa are inoculated by VA mycorrhizal fungi could make P. ussuriensis rhizosphere nutrient enrichment.

大青杨与紫穗槐混交并接种丛枝菌根真菌后，可使大青杨根际营养&富集&。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。