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Coll or MPAIV alone.the group inoculated with MPAIV and E.coli was more severe inflammation of the trachea, lung, and air sac and dispersion necrosis of parenchyma organs than that of groups inoculated with MPAIV or E.coli alone.

迅速，细菌数目也比A组多，l2 细菌数目差异显著，在3h后差异不显著。④各组脾脏。肾脏和肝脏在接种后偶尔可以分离到细菌，而且数量很少。

In the mycorrhizal inoculation test, the total dry weight of inoculated coneflower was more than control. The concentration of cichoric acid in the leaves of inoculated coneflower, 472.520 μg/mL was significantly higher than uninoculated control 245.638 μg/mL.

在菌根菌接种实验中，接种菌根菌的紫锥菊总乾重比未接种菌根菌的紫锥菊高，实验中也发现VA组叶子cichoric acid浓度为472.520

To study the cytopathogenic effect of mycoplasma membrane.the pro- toplasmic membrane of the mycoplasma causing swine enzootic pneumo- nia was prepared by the repeated Freeze-thaw method or the osmotic shock method,and was then inoculated into the rabbit kidney primary tissue cultures for examining their biological activities.The experimental results were as follows:in the cultures inoculated with 25～50μg of membrane protein ,the cytopathogenic effect was considerable.in the cul- tures inoculated with ...

用低渗解体及反复冻融裂解的方法，制备了猪喘气病病原霉形体膜，将所制备的膜制品接种兔肾原代单层细胞，作生物学活性观察，在接种量按蛋白含量计算为25～50微克膜蛋白的细胞管中观察致细胞病变作用，在接种17.5微克膜蛋白的细胞管中作用不明显，接种6微克膜蛋白的细胞管无致细胞病变作用。

Results The serum antibody level reached to 0.71±0.08-0.77±0.06 after genetic vaccine pCD-HCV1(100 μg/mouse) were inoculated into the mice(n=12) three or four times while blank vector pCD-SRα1 could not induce the mice(n=8) to generate antibody response in same way.After the antibody levels in mice(n=8) immunized by pCD-HCV1 had ascended to peak value(0.71), there was no trend of descending during the following 18 weeks of detection(0.68±0.06-0.75±0.07). Specific fragment of HCV cDNA identified by polymerase chain reaction from DNA extracted from the muscles of the mice after pCD-HCV1 had beed inoculated three months.

结果 基因疫苗pCD-HCV1(100 μg/只)3-4次接种小鼠后(n=12)，血清抗体水平达0.71±0.08-0.77±0.06，空载体pCD-SRα1免疫鼠血清抗体阴性；免疫鼠(n=8)抗体水平达高峰后(0.71)，持续检测18周未见下降趋势(0.68±0.06-0.75±0.07)；pCD-HCV1免疫小鼠3个月后，用聚合酶链反应仍可从肌肉组织中扩增出特异性HCV cDNA片段；免疫鼠PBMC对HCV合成肽CP9、基因重组抗原C、E1刺激后均出现增殖反应，刺激指数分别为4.07±1.58、3.88±0.70和3.69±1.30，与pCD-SRα1免疫鼠相比，差异有显著性(P.001)。

The plantlets of soybean, cucumber and garden balsam were inoculated by wild-type Agrobacterium rhizogenes K599, and hairy root was induced on inoculated sites in vivo. The frequencies of hairy root induction from wound cotyledons of soybean, cucumber and garden balsam were 100%, 65% and 91%, respectively.

利用野生型的发根农杆菌K599对属于不同科属的大豆、黄瓜和凤仙花进行活体感染实验，结果表明，发根农杆菌K599可使切割后的大豆、黄瓜和凤仙花子叶形成不定根，生根频率分别为100%、65%和91%。

The tumorigenetic rate in inoculating with 5×105 B16 cells was near 100%CONCLUSIONThe tumorigenetic rate has a positive relation to the number of inoculated B16 cells, and is high in BALB/c mice inoculated the B16 cells into back hypodermis.

在接种细胞数大于5×105个时BALB/c小鼠的肿瘤模型全部建立成功［结论］B16细胞株黑色素瘤细胞接种于BALB/c小鼠的肿瘤模型成功率与接种细胞数成正比，它具有建立肿瘤模型成功率高，观察及测量方便等优点，接种部位以背部皮下为宜。

All leguminous seeds were inoculated with specific Rhizobia in 1999 but SC and V were not inoculated in the last years of the study.

所有豆科植物种子接种根瘤菌在1999年与具体但SC和V没有接种在过去几年的研究。

After cultivated, tube plantlets weren't evidently different compared with the control (inoculated under no NaClO, free virus conditions) in main growth indexes,which showed that Oncidium' s tube plantlets could be inoculated under open conditions when film culture vessels were used.

培养后的试管苗在主要生长指标方面与对照(不添加NaClO、无菌条件下接种)相比无明显差异，表明采用树脂膜培养容器可以在有菌条件下进行文心兰试管苗的接种。

KM strain mice immunosuppressed with 7. 5～10mg/L DEX in drinking water had the higher oocysts output and fewer dead than other groups.(4) There was no significant difference infection rate between groups inoculated with different number CSO and groups inoculated CSO with conserved different time. Trial were divided into control, groups of 50mg/kg /d, 100 mg/kg /d and 200 mg/kg /d dose of the drug to study the anticryptosporidial activities of nitazoxanide in an immunosuppressed mice model. The experimental results showed the treatment with 200mg/kg bw /day was significant inhibition of oocyst excretion. To detect the two cryptosporidiums strain C.

实验结果表明：C.parvum虫种能够感染上本实验所采用的BALB／c和昆明小白鼠两种小鼠，而C.andersoni没有能够感染本次实验小鼠；且从小鼠感染后的排卵囊量和小鼠的生存状态来看，采用5～6周龄的KM鼠能获得最大量的隐孢子虫卵囊，小鼠的死亡数最少；每升饮水中地塞米松量为7.5mg～10mg组比12.5mg以上组小鼠死亡数量少，比5mg／L以下组排卵囊数量多；接种不同卵囊数和卵囊的保存时间这两个参数均对小鼠受感染后的排卵囊情况影响不大。

NJWGY3665 bacterial strain is inoculated in a glucose nutrient culture medium to carry out the slant culture, the culture temperature is 28 to 37 DEG C and the culture time is 2 to 6 days;(2) seed culture: a spore which is cultured on a slant is produced into a single-spore suspension by using sterile water, which is also inoculated in a seed culture medium for culture, the temperature is 28 to 32 DEG C, the rotational speed is 200rpm and the culture lasts for 2 to 6 days;(3) fermentation culture: the seed liquid is inoculated in a fermentation culture medium for culture, the temperature is controlled at 28 to 37 DEG C, the pH is controlled at 6.0 to 9.0, the rotational speed is 180 to 220rpm, the fermentation lasts for 5 to 9 days, so as to obtain the Streptomyces sp., methanol or ethanol is used for the extraction and separation of mycelium through the method of centrifugal separation, then the resin method is adopted for carrying out refining, so as to obtain the peptide antibiotics enramycin.

NJWGY3665菌种接到葡萄糖营养培养基中，进行斜面培养，培养温度28-37℃，培养时间2-6天；(2)种子培养：用无菌水将斜面上培养的孢子制成单孢子悬液，并接种到种子培养基中培养，温度为28～32℃，转速为200rpm，培养2-6天；(3)发酵培养：将种子液接种到发酵培养基中培养，温度控制在28-37℃，pH控制在6.0-9.0，转速180-220rpm，发酵5-9天，得到链霉菌，采用离心分离的方法将菌丝体用甲醇或乙醇进行提取分离，再采用树脂方法进行精制，获得多肽类抗生素安来霉素。

The concept of equivalent rotationally rigidity is offered and the formula of rotationally rigidity is obtained.

主要做了如下几个方面的工作：对伸臂位于顶部的单层框架—筒体模型进行分析，提出了等效转动约束的概念和转动约束刚度的表达式。

Male cats normally do not need aftercare with the exception of the night after the anesthetic.

男猫通常不需要善后除了晚上的麻醉。