查询词典 inoculate

In order to develop a safe and effective immunoadjuvant to enhance the immunity and resistance of animals against infection, a novel CpG Oligodeoxynucleotides containing 11 CpG motifs was synthesized and inserted into the VR1012 plasmid, designated as VR1C. Then the recombinant VR1C was entrapped with Chitosan nanoparticles prepared by the method of ionic cross linkage, and employed to inject muscularly 3-weeks old Kunming mice; the blank VR1012 packed with CNP and saline were used to inoculate mice as the control groups. 28 days after inoculation, all mice were orally fed with 0.4ml 2x108CFU/ per mouse virulent hemorrhagic enteritis E. coli to challenge the resistance against infection.

为研制安全高效免疫调节剂增强动物免疫抗病能力，本实验设计合成含11个C pG基序的寡聚核苷酸，重组构建含CpG的VR1012质粒(VR1C)；制备壳聚糖纳米颗粒包裹重组质粒(CNP-VR1C)，肌注接种3周龄昆明小白鼠，设壳聚糖包裹空质粒和生理盐水对照组；接种后28天口服大肠杆菌攻毒观察小鼠天然免疫的变化和对强毒感染的抵抗力，Sandwich ELISA测定血清免疫球蛋白和白细胞介素含量。

The results showed:among the 7 isolates, five isolates of HY3、GY1－3、ZJ1－1、HP1、FC3 had same colony shape, irregular shape, liquidlike, slimy, opacity with smooth surface;the other two isolates had same shape, irregular shape, dry, opacity with coarse surface. By inoculating eucalyptus with the 7 isolates, the plants were infected apparently, and the young plants of eucalyptus in control experiment with tap water were not infected. By cultivating eucalyptus cuttings with the bacterial suspensions without EPS, the incidence of disease was very distinct,but compared with the former bacteria suspension,the incidence of disease has decreased at different degrees. By screening out two isolates of strong pathogenicity and two isolates of weak pathogenicity from the 7 isolates,making the bacterial suspensions with them to inoculate the young plants of eucalyptus, two treatments of cutlings and ramets with rats were set with 5 repetitions in every treatment, the results of data analysis showed: for the cutlings, the bacterial contents in upper and middle parts、upper and lower had significant difference;for ramets with roots, the bacterial contents in upper, middle parts, lower had significant difference between each other; For both the cutlings and ramets with roof, the bacterial contents in xylem and phloem had significant difference. The interaction between vertical and horizontal parts for the bacterial content had significant difference. For the two isolates of HY3 and 93B which were screened out at last,their activities of the cellulase were: 1.955ug/ and 1.288ug/ respectively, and had significant difference; the activities of pectase were: 1.325 ug/and 1.24ug/ respectively, and had no significant difference. The content of EPS extracted from the two isolates of HY3 and 93B was very different: 7.08x10-8ug/cell and 5.17x10-8ug/cell.

结果显示：7个菌株中，其中5个菌株HY3、GY1－3、ZJ1－1、HP1、FC3的菌落形态相同：不规则形状、流体、粘性、不透明、表面光滑；另外2个菌株93B、GN1菌落形态相同：不规则形状、干燥、不透明、表面粗糙；用7个菌株接种剪根桉树苗，发病情况非常明显，而自来水对照实验中桉树苗却不发病；无EPS菌悬液培养桉树剪根苗，发病率也很明显，但是相比原菌液，则发病率有不同程度的下降；从7个菌株中间筛选出来2个强致病性菌株和2个弱致病性菌株，用它们配制菌悬液培养桉树苗，设置剪根和不剪根两个处理，每个处理设置五个重复，数据分析结果显示：对于剪根苗，上部和中部、上部和下部的含菌量有显著的差异，中部和下部含菌量差异不显著；带根苗，上部、中部、下部含菌量彼此之间差异显著；不管是剪根苗还是带根苗，木质部和韧皮部含菌量之间的差异都非常显著；上中下与木韧交互作用中，含菌量差异非常显著；最后筛选出来的强弱2个菌株HY3和93B，它们的纤维素酶活性分别为：1.955ug/和1.288ug/，具有显著的差别；果胶酶的活性分别为：1.325 ug/和1.24ug/，没有显著的差别，而且HY3和93B两个菌株细胞分泌的胞外多糖的含量差异很显著，分别为：7.08×10-8ug/cell和5.17×10-8ug/cell。

These ingredients includes epidermal growth factor, hepatocyte growth factor, dimethyl sulfoxide, phenobarbital sodium and all trans-retinoic acid. We isolate and culture primary 10-weeks-old human fetal hepatocytes, observe hepatocytes form and its function markers. Inoculate hepatocytes with HBV Dane particles to observe if HBV susceptibility of fetal hepatocytes can be induced in vitro. To detect HBV covalently closed circular DNA within hepatocytes, we improve the existent PCR method.

基于上述假设，我们设计了10种培养条件，在自行设计的无血清培养基中添加不同的细胞分化诱导物，包括表皮生长因子、肝细胞生长因子、二甲基亚砜、苯巴比妥钠、全反式维甲酸等，分离孕10周龄人胎肝细胞进行原代培养，观测肝细胞形态及功能指标变化，并定时给予HBV感染，观察能否在体外诱导胎肝细胞出现HBV易感性；为检测肝细胞内HBV共价闭合环状DNA(covalently closed circular DNA，cccDNA)，我们改进了现有的PCR检测法。

In the chance of Qing Terminal New Deal, Japan attracted Chinese young people to study in Japan so as to bring up emotion to Japan, and attempted to inoculate these ridgepoles with asianism and militarism. Besides, Japan dispatched flock of counselors, teachers and technologists. Many of these people were espionages, they received orders from the Japanese government, and they butt in Chinese affairs and detect information as teacher or consultant.

为了不使中国在被日本独霸之前被其它国家瓜分，日本打着&保全中国&的幌子，以帮助清朝新政为契机，吸收中国学生到日本留学，培养中国人的亲日西南交通大学硕士研究生学位论文第日页感情，并企图对这些国家栋梁灌输亚洲主义、军国主义等思想；此外，还向中国派遣大量的顾问、教习和技术人员，其中大量谍报谋略人员身负重任，以教习顾问的身份为掩护，插手中国事务，探取中国的情报，并妄图以日本的方式&改造&中国。

But Vitamin and inoculate period have little effects. The HPLC analysis results indicate there is no PSP in Gymnodinium spp., a little PSP of 10.81 fmol /cell in Alexandrium catenella.

HPLC检测分析藻细胞乙酸提取物得到以下结论：裸甲藻细胞内不含有PSP，而亚历山大藻细胞内含有PSP且每细胞含量为10.81fmol／cell，易形成有毒赤潮。

By determining the extract to the chicken embryo's maximum lethal concentration and hemagglutination. By different medicine liquor concentration direct action in virus, inoculate embryo determine the change of virus titer, found that all 8 compoundings have the effect of antivirus, and Mahuang is the essential component.

测定提取液对鸡胚的最大致死浓度和对红细胞的凝集作用；以药液的不同浓度直接作用于病毒，接种鸡胚检验病毒滴度的变化，观察发现8种配方都有抑制病毒的作用，其中麻黄是发挥抗病毒作用的主要成分。

The first method adopts different kind microbes to ferment buckwheatextraction. The method can increase DCI and its derivation content in buckwheat seed solution, while itreduces sugar content in extraction. The research adopts Saccharomyces cerevisia, Mucor, Bacterium coli, Torulopsis apicola, Aspergillus oryzae Bacillus subtilis, Candida mycoderma, Black mold that inoculate inbuckwheat extraction. Determine content change of various compotent with tracing.

方法一采用不同种类微生物发酵荞麦提取物，增加荞麦籽粒水溶液中D-手性肌醇及其衍生物的含量，同时减少提取物中总糖含量，本研究采用酿酒酵母、毛霉、大肠杆菌、蜜蜂生球拟酵母、米曲霉、枯草芽孢杆菌、解酯假丝酵母、黑曲霉接种于荞麦提取物中进行培养，并跟踪测定提取物中各种成分的含量变化。

METHODS:The gene of human ET1 was synthesized according to the preferential codons of E. coli, cloned into the EcoRI and SalI sites of vector pThioHisA. The recombinant plasmid pThioHisA-ET1 was constructed , sequenced and transformed into E.coli TOP10. Induced and expressed fusion protein were identified and analysed by 12% SDS-PAGE and densitometry analyses. After the elution, denature and renature, the fusion protein Thioredoxin-ET1 was obtained by ProBondTM chromatogragraphy. The purity of Thioredoxin-ET1 was detected by HPLC. Inoculate Thioredoxin-ET1 once per mouse every 2 weeks in 25, 50 and 100μg separately on 3 groups for 4 times. 10 days after last inoculation, we obtained venipuncture blood.

根据人ET1的多肽序列合成ET1基因，将其插入到pThioHisA的EcoRI和 SalI位点，重组质粒pThioHisA-ET1进行酶切鉴定及序列测定验证后转化TOP10，IPTG诱导的重组菌经SDS-PAGE检测融合蛋白Thioredoxin-ET1的表达量；表达的融合蛋白用ProBond亲合层析纯化并经HPLC鉴测其纯度；每只小鼠按25、50、100ug/次剂量的Thioredoxin-ET1每两周免疫一次，共4次，最后一次免疫10d后制备抗血清，经Western blot和ELISA检测证明Thioredoxin-ET1融合蛋白具有ET1免疫反应原性。

Inoculate a single colony in BMGY medium, grow at 30 °C with 250 r/min until culture reatch an OD6oo=2.0. Harvest the cells by centrifuging at 1,500 r/min for 5 min at room temperature. Resuspend cell pellet to an OD6oo=10 in BMMY medium to start induction.

将经过酶切鉴定和测序正确的阳性克隆电转入毕赤酵母GS115中，挑取酵母转化单菌落，接种于BMGY培养基，30℃、250r/min培养至OD_(600)=2.0时室温1，500r/min离心5min，用BMMY培养基重悬菌体使OD_(600)=1.0，重新放入摇床进行诱导。

Suspensions of field samples suspected to contain FMD virus should be clarified and inoculate d into cell cultures or unweaned mice.

将可疑含FMD病毒的田间样品酊悬浮液经澄清后接种于细胞培养物或未断奶乳鼠。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。