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While compared with routine linearity injection, arc-needle injection 131I-iodinated oil step-by-step sector-shapely through implant C6 glioma and SMMC-7721 hepatoma at subcutis, Rat survival time increased, the body weight kept less descent, their lumps necrose wholely.

对C6、中文提要放射性核素在瘤体内的均匀弥散、持续滞留和辐射剂量估算研究 sMMc一7721的皮下瘤模型瘤内弧形针分段扇形注射'3,I一碘化油,与直行针线状注射相比,动物存活时间长,体重下降少,肿块皇整体坏死。

Healthy SD male rats were given DEG with the dose of 8 mL/kg body weight (intraperitoneal injection I.P.). The level of the serum Cr, BUN and CO2CP(CO2 Combining Power CO2CP)were tested before and after the injection the 2nd , 5th , 7th, and 14th day. The nephridial tissue of all the rats was taken for pathological examinations.

选用健康雄性SD(Sprague-Dawley)大鼠,腹腔注射8 mL/kg的DEG,于注射前和注射后第2天、5天、7天以及14天尾静脉采血检测血肌酐、尿素氮和CO2结合率(CO2CP),收集死亡大鼠及第7天和14天处死大鼠的肾组织进行病理检查。

TyPe II collagen induced arthritisln the rat ank1e joint andoVathumin as antigen induced arthritis WA in the rabbit knee joint wereestab1ish2 Qualitative evaluation of me in skin, muscle, synovium, cedilagearound joint and blood was performed by OMA3 The CIA rats were treated on day 7 after hind paw swelling and erythemaAnimals were injected intravenously with ase at a dose of 10mg/kg,tWenty minuots 1ater, one ankle of the rats random1y assigned was exPosedlaser irradiation at l00J/cm fOr l000 seconds, and another ankle wasM grouP wihout laser The other two groups is unmanipulatedcontrol group and untreated CIA group Bimaleolar ankle widthmeasuremellts were taken in all animals every tWo days using amicrometer The histopathology of the ank1e Joint was assessed at day 21after disease onset4 The pro1iferating cell nuclear antigen WCNA of CIA treated by PDT andthe HMME group without laser was doterdrined by immunohistochemiStry5 The AfA rabbits were treated on day 7 after knee swelling and erythemaThe theraPy invo1ved lntravenous injection of l0mg/kg HMME, fOl1owedby 20 minues period in dim light, and transdermal light treatment with\l00 J/cm2 fOr l000 seconds The inner sides of the treated Anees wereirradiated at first, and then the outer side did 24 hours later, the synovialtissue of the Anees joint were removed and in situ cel1 aPoptosis wasdetCCted With tednal deoxync1eotidyl transferase-mediated dUTP nickend labelingR6suIt8:l The pathologic changes of CIA and AIA include subsynovial inflammation,opovial hyPerplasia, pannus formation, cartilage and bone destructionresemble RA.2 The studies demonstrated that there are different uptake of HMME withinskin, muscle, synovium, cartilage and b1ood, and the synovium cou1draPidly uPtake more ase than skin and cartilage at the firSt 30 minuesaller intravenous injection of HMME3 The bimaleolar anke width had no different among PDT treated group,H group withollt 1aser and untreated CIA group But hlstologicalevaluation showed statiStical1y significallt reductions in synovialhyperplasia, pannus formation and cart1lage reosion, bone destruction andtotal score in PDT treated group4 Image analysis showed that the ratlo bforeen the areas of the coufltedobect to that of the entire area in PDTtreated grOup is lower than that in conirol group, but the integrated oPticaldensity had no different between the two groups5 Imape analysis showed that the ratio between the area of the countedobject to that of the e

治疗组在大鼠出现踝关节红肿后1周,炎症达到高峰时进行PDT治疗。随机治疗大鼠一侧的踝关节,另。2。一一侧作单纯HMME 对照。治疗方法是大鼠麻醉后尾静脉注入 HMME10ngkg,20分钟后踝关节照光,激光波长627.sum,功率密度 100mwcm',照射时间1000秒,能量密度100)/。治疗后避光喂养72 小时。隔日一次测量大鼠的踝关节左右横径,治疗后两周取关节进行病理d 观察。 4。大鼠CIA模型用上述方法进行PDT治疗后,治疗组和单纯HMME 组用兔疫组化SP法检测石蜡切片的核增殖抗原。 5。兔AIA模型在关节炎出现第七天进行PDT治疗,随机治疗一侧膝关节,另一侧作自身对照。兔耳静脉注入I'arrainrelomg/Kg,20分钟后,膝关节用金蒸气激光照射,激光能量密度100)儿旷。24 /J'时后取膝关节滑膜作病理检查,并用脱氧核昔酸末端转移酶介导的缺口末端标记法原位检测凋亡细胞。结果: 1。模型观察:CIA大鼠炎症高峰期滑膜下炎细胞浸润明显,滑膜细胞明显增殖,炎症达到高峰后二周,血管缀形成,并侵蚀和破坏软骨和骨, CIA模型病理改变与人类RA相似。兔AIA模型膝关节滑膜病理可见滑膜细胞增生,滑膜下炎细胞浸润,也与人类RA滑膜改变相似。 2。关节周围组织中光敏剂含量的测定结果表明,各组织对HMME 的吸收速度和吸收量不同,荧光值一时间曲线不同,滑膜组织比皮肤和软骨对 HMME的吸收多,在 2 0分钟时即有明显差异。 3.PDT对CIA模型的治疗结果表明:PDT治疗后关节炎组、单纯 HMME组和治疗组踝关节左右横径统计学检验差异没有显著性,但病理评分PDT治疗组滑膜增生、血管资形成及软骨破坏、骨破坏和总分比关节炎对照组和HMME对照组好,统计学检验差异有显著性。。3_军医进修学院硕士学位论文中文摘要 4.PDT治疗组PCNA阳性细胞较对照组少,图像分析结果表明面密度(阳性染色的面积总和与统计视野面积的比值)治疗组小于对照组,统计学检验差异有显著性。。 5.PDT治疗组凋亡阳性细胞较对照组明显增多,图像分析结果单位视野内阳性细胞数和面密度PDT治疗组高于对照组,统计学检验差异有显著性。凋亡细胞核直径PDT治疗组较小,与对照组相比,统计学检验差异有显著性。结论:二。CIA、AIA的病理改变类似人类RA,可作为研究RA病因、发病机制、检查及治疗方法的模型。 2。各组织对HMME的吸收速度和吸收量不同,滑膜组织比皮。

The microcirculation disturbance in mice was brought by rapid injection of adrenaline.The micro vascular diameter,the blood flow speed and micrangium crossing net patefaction number in auricle of mice at normal condition and in the 10,20,30 min after injection of adrenaline were observed by microcirculation microscope with analytical system.

连续给药4 d,末次给药30 min时将小鼠固定于微循环观测分析系统显微镜下,观测小鼠正常及腹腔注射盐酸肾上腺素(1 mg·kg-1)10、20和30 min时小鼠耳廓微血管的管径、血流速度及毛细血管交叉网开放数目。

Electroencephalographys were detected in SE and SC groups after 2 h of drug injection. Long term potential at perforant pathway and dentate gyrus and EEG were recorded in LE and LC groups after two weeks of drug injection.

注射药物2 h后,SE和SC组检测脑电图;药物注射后2周,LE和LC组检测内嗅区前穿通纤维-海马齿状回长时程增强和EEG。

Methods: Sixty-four patients undergoing total knee arthroplasty were randomized either to receive a periarticular intraoperative injection containing ropivacaine, ketorolac, epimorphine, and epinephrine or to receive no injection.

将64位需行全膝关节成形术的患者随机分为两组,一组在术中接受局部药物注射,药物组成包括ropivacaine、 ketorolac、epimorphine和epinephrine;另一组术中不做局部注射止痛。

The effect of a vitreous injection of the caspase-3 inhibitor Z-DEVD-FMK lasts longer than the effect of a peribulbar injection.

Caspase-3抑制剂Z-DEVD-FMK的玻璃体腔注射可能比球周注射作用更加持久。

Objective To approach the therapeutic effectiveness of early intervening treatment of diabetic footwith ciwujia injection and decoagulant.Methods 100 diabetes mellitus with early pedopathy was divided into treatment group and controlled group with the ADA diagnostic criteria in 1997. 50 patients of the treatment group were subjected to intervening treatment with ciwujia injection and peroral decoagulant(persantine、enteric-coated aspirin), while 50patients of the control group received peroral VitB1、VitB6、complexdanshen.

目的 观察刺五加注射液及抗凝剂对糖尿病足早期干预治疗的影响方法将符合1999年WHO诊断标准的100例糖尿病合并早期足病变的患者分两组:干预治疗组50例,对照组50例,在控制血糖、血压、糖尿病教育的基础上,干预组予以静滴刺五加注射液及口服抗凝剂治疗,对照组为口服VitB1、VitB6、复方丹参片治疗。

An experimental research on protective effect of compound injection of Salvia miltiorrhiza by retrobulbar injection in photic injury rat retinopathy.

枸杞在保护大鼠视网膜光损伤中作用的研究。

The male and female rats (n=15, respectively) were randomly divided into three groups, and Fluoro-Ruby, a fluorescent tracer, was stereotaxically injected into the ventromedial hypothalamus, bed nucleus of the stria terminalis and posterodorsal nucleus of the amygdale by iontophoresis, respectively.Five days later, the rats were perfused, and the sections containing SDN-POA region and site of injection were cut on a freezing microtome.the sections were viewed to assess extent of tracer deposition in the injection site and to reveal the EGFP or Fluoro-Ruby fluorescence in SDN-POA under fluorescence microscope.

雌、雄性大鼠(各15只)随机各分成3组,分别通过离子电渗法将示踪剂Fluoro-Ruby注入下丘脑腹内侧核(VMH、终纹床核、杏仁核后背侧核。5 d后,灌流固定鼠脑,做冷冻冠状切片,荧光显微镜下确定示踪剂注入位置,并观察SDN-POA绿色荧光和Fluoro-Ruby标记荧光。

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