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Some injected oocytes were activated by Sr(superscript 2+) after various incubation periods and observed for formation of a second polar body and pseudo-polar body.

在注射后的不同培养时间，对重构卵子以Sr(上标 2+)激活，观测第二极体和类极体的形成，同时对重构胚进行染色体计数，并将发育良好的重构胚进行输卵管移植。

It is found that the limited maximum number of injected Li+ ions is predicable through the variation trendaney of interface potential.

通过实验发现：界面电位差的变化趋势能够反映出氧化钨薄膜的Li+离子阈值注入量的大小。

AIM: To establish a transgenic mouse model of Hi1tchirtson-Gilford progeria syndrome and observe the effect of mutant lamin A on the implantation and survival ability of injected mouse zygotes.

目的：制备Hutchinson-Gilford早老症转基因小鼠模型，观察突变的核纤层蛋白A对小鼠受精卵着床和成活情况的影响。

In our experiment, we found two abnormal phenotypes that one only the last three segments develop into pupa, but the other segments are still keep the larva characters; another sample has differently develop speed between abdomen and backside, which resulted in the back develop into pupa but the abdomen were still the larva characters. When we add Az in the feed of injected EcR-dsRNA samples, in the 13 live larvae we found that 6 have the abnormal proleg phenotypes.

在另一组注射EcR-dsRNA以后通过浸叶法添食不同浓度的印楝素，在10ppm处理组存活的13个个体中有6个出现特异表型，其中两个幼虫为不完全蜕皮，尾部体节无法蜕皮导致发育受阻；另外发现4个供试虫体发育至五龄时出现腹足缺失表型，其中有两个是腹足几乎全部缺失，腹足应该生长的部位只有少数几个突起，没有发育成形的腹足；一个出现左侧腹足完全缺失，右侧第二腹足缺失表型；另一个个体只缺失了左侧最后一个腹足。

In some cases, the height of vertebra could be increased after the operation, however, it is not for the technology but for the posture of patients that the patients would pronate on the table and over-straight their bodies. In this situation, bone cement can be injected into the vertebras and enhance the int ensity, change the spine kyphosis, recover the spine physiological line, so it gets to the aim of treatment.

有时病椎术后高度增加，不是椎体成形术操作本身抬高椎体高度，而是在病人俯卧位时进行的体位引起过伸而复位，此时椎体内再加入骨水泥固化，使椎体固定在复位后的位置上，并且增加了耐压强度，同时改变了脊柱的后凸畸形，恢复了脊柱生理力线，这样就会达到治疗目的。

To make the transgenic mice: We purified the gene fraction nestin-hsp68-EGFP and injected it into pronucleus of mouse zygotes to generate the transgenic mice.

1。转基因小鼠的获得：纯化基因片段nestin-hsp68-EGFP，用显微注射方法把基因片段注射到受精卵的雄原核中，获取转基因小鼠。

Oocytes injected with frozen-thawed sperm were activated by various methods, and the effect on the pronucleus formation and blastocyst formation rate were examined.

方法］冷冻精子解冻洗涤后直接应用于ICSI，ICSI后采用不同激活方法激活卵母细胞，检测其原核形成情况。

Then the linear vector was microinjected into the pronucleus of the KM×B6D2F1 zygotes to produce the transgenic mice. Thirty-four adult mice were produced from 286 of injected embryos.

用显微注射方法，将线性化的载体DNA注入KM ×B6D2F1小鼠受精卵的的原核内部，然后将胚胎移植到输卵管内，13只受体怀孕产仔。

The concentration of the fragments was 2ug/ml, the copy number in lml was about 2.4x10, every 1 to 2pl of the prepared DNA solution was injected into the embryos at pronucleus stage .

以人肥胖基因cDNA为探针，用EcoRI消化总组织DNA，经Southern杂交反复确证其中两只母鼠(2~#与C_3)为转人肥胖基因小鼠。

Lamin A gene cDNA lacking of 150 basyls at the end of the eleventh extron was cloned into pcDNA3.1 carrier, and the recombinant plasmid pcDNA3.1-mutant LMNA cDNA was injected into the male pronucleus of mouse zygotes, then the zytotes was replanted into the oviducts of pseudopregnant mice.

实验方法：将缺失了第11外显子末端150个碱基的LMNA基因cDNA克隆到pcDNA3.1载体，应用原核显微注射方法将线性化的pcDNA3.1-mutant LMNA注射到小鼠受精卵的雄原核，再将注射后的受精卵移植至假孕鼠的输卵管。

The work of this paper is as follows: 1. Looking back the progressing history of the linear motor, introducing the features of the elevator driven by linear induction motor, radicating the topic of this paper "the digital frequency variable control of the elevator bi-side direct driven by linear induction motor". The research of this paper covers the conventional VVVF control, space vector based VVVF control, vector control and DTC.

本文主要开展了以下几个方面的工作： 1 回顾了直线电机发展历史，电机的驱动技术演变，特别是针对直线电机的驱动，简要介绍了直线感应电机驱动电梯的优点和不同结构类型，对传统的v／f控制，基于空间矢量法的v／f控制，矢量控制，和基于电压空间矢量的直接转矩控制进行了比较，确立了本课题的研究主题：直线感应电机双边直推式驱动电梯的全数字变频控制。

The article combines with the treatment of a superficial civil air defense work to introduce how the grouting method to improve the performance of the backfill soil.

文章从治理漂浮人防工事的角度提出了注浆技术在改善回填土性质方面的应用，并详细阐述了注浆技术的施工流程。