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In the first group, photosensitizer was injected intravenously quickly before irradiation as in the previous treatment. In the second group, photosensitizer was injected intravenously slowly throughout the first 15min of irradiation. In the third group, hot fomentation was made on the treated area for 30min, then photosensitizer was injected quickly as in the first group.

3建立PWS组织光学模型，采用Monte carlo方法模拟了临床常用532nm激光在PWS组织中的分布特点，分析皮肤各层组织对其吸收的特点、组织结构(表皮黑色含量、病变血管深度等)以及治疗中氧含量变化对光能流率在PWS组织中分布的影响。

Methods The tissues of cervical carcinoma with HPV16/18positivity was injected into the hypodermis of nude mice to make tumors and transmission,then Ad-p53gene was injected into the tumors to investigate the effect,while DDP,BLM and normal saline were injected into the tumors to as control groups.

将含HPV16/18型感染的宫颈鳞癌组织注入裸鼠皮下成瘤、传代，将以缺陷型腺病毒为载体的P53基因（Ad-P53）瘤内注射，同时用抗癌药DDP、BLM以及生理盐水作对照，观察P53基因治疗宫颈癌的有效性。

Methods The recombinant sequence for two CEA epitopes was tandemly engaged and inserted into-upstream of in-frame-varied HSP70 of Mycobacterium tuberculosis to construct a gene vaccine.Balb/c mice were muscularly injected with recombinant DNA vaccine;negative control (mice were injected with normal saline), positive control (mice were injected with DNA vaccine harboring tandem CEA epitopes plus aluminium hydroxide adjuvant) and experimental group(mice were injected with PCITri CEA625-667-met HSP70) were set up.

在已经构建含有变异热休克蛋白序列的基础上，插入重组CEA串联表位的编码片段获得CEA串联表位-HSP融合基因疫苗。3次肌肉注射免疫Balb/c小鼠，设立注射生理盐水的阴性对照组、注射氢氧化铝佐剂混悬CEA串联表位的阳性对照组及注射pCITriCEA625-667-mtHSP70的实验组。

MethodsNonheparinized autohemic blood of the rat was taken out from rat"s femoral artery and injected into its caudate nucleus immediately to establish a model of intracerebral hemorrhage.The changes of edema in the ipsilateral bassal ganglia , ipsilateral cortex, contralateral bassal ganglia and contralateral cortex were measured at 1,2,3,5 and 7 days respectively after intracerebral hemorrhage. The rat"s packed red blood cells, lysed red blood cells and hemoglobins at different concentrations were injected respectively into the rat"s caudate nucleus. The changes of edema in various regions of the brain at different time were measured as above. 5U thrombin was injected into the rat"s caudate nucleus and the changes of edema in various regions of the brain at different time were measured as above.

自体股动脉取血，并采用自体未肝素化新鲜血回注法建立大鼠尾状核出血模型，观察脑出血后1，2，3，5，7天血肿同侧基底节区、血肿同侧对应的皮质区、血肿对侧对应的基底节区及血肿对侧对应的皮质区脑组织水分含量的变化；分别以自体浓缩红细胞(PRBC，50％)、溶解红细胞(LRBC，50％)、不同浓度的大鼠血红蛋白注入大鼠尾状核，并观察不同时间不同部位脑组织水分含量的变化；以5U凝血酶注入大鼠尾状核，观察不同时间不同部位的脑组织水分含量的变化。

Fifty children undergoing arm operation were randomly divided into two groups:After ketamine based anesthesia,Plexus brachialis nerves block combined with ketamine propofol intravenous anesthesia(group A, n=25), 0.25％ropivacaine 0.5ml/kg was injected to plexus brachialis,ketamine propofol was injected intravenous connected during the operation;Ketamine propofol intravenous anesthesia(group B,n=25),ketamine propofol was injected intravenous connected.

50例行上肢手术患儿随机分为两组：氯胺酮基础麻醉后，臂丛神经阻滞复合氯胺酮－丙泊酚静脉麻醉组(A组，n=25)，臂丛阻滞成功后注入0.25％罗哌卡因 0.5ml/kg，术中以氯胺酮－丙泊酚持续静注；单纯氯胺酮－丙泊酚静脉麻醉组(B组，n=25)，术中氯胺酮－丙泊酚持续静注。

GdCl3(10mg/kg) or the same volume of NS was continually injected of vein at 48h and 24h before LPS(5mg/kg) was injected in the male kunming species mouse. Then took out of liver or isolated KCs 30 minute after LPS was injected and assay the protein expression and phosphorylation level of ERK1/2 and p38MAPK of liver or KCs in vivo.Ⅱ. Isolated and cultured KCs of mouse, 1 h pretreatment with GdCl3; Culture medium with LPS(100ng/ml) was added and continuatively incubated 30 minute. Assay the protein expression and phosphorylation level of ERK1/2 and p38MAPK of KCs in vitro and detection effect of GdCl3 on its phagocytosis function in respectively.

Ⅰ 雄性昆明种小鼠在注射LPS（5mg/kg）前48h、24h，分别静脉注射GdCl3（10mg/kg ）或等量的生理盐水，于LPS或生理盐水注射后30min，分别取出肝脏或分离KCs，检测肝脏或KCs ERK1/2、p38MAPK蛋白表达及磷酸化水平；Ⅱ体外培养小鼠KCs经GdCl3（100μM）预处理1h，加入含LPS（100ng/ml）的DMEM培养基继续孵育30min，分别检测体外培养KCs ERK1/2、p38MAPK蛋白表达及磷酸化水平和GdCl3对其吞噬、分泌功能的影响。

Thus, the analgesic effect of morphine injected into PAG was significantly attenuated by β-EP AS injected into the nucleus accumbens, and vice versa. This antagonistic effect was totally abolished by lesioning of the ARH. Moreover the analgesic effect of morphine injected into nucleus accumbens could be blocked byβ-EP AS administered te amygdala or habenula.

在PAG内注射吗啡产生的镇痛作用可被伏核内注射β-EP抗体所削弱，损毁家兔的ARH后这一对抗效应基本消失；在伏核内注射吗啡产生的镇痛作用可被PAG内注射β-EP抗体所对抗，损毁ARH后上述对抗效应基本消失；伏核内注射吗啡产生的镇痛作用亦可被杏仁核内或缰核内注射β-EP抗体所阻断。

Two hundred 16-day-old of age commercial broiler chickens were randomly divided into 4 groups as follows: 50 birds were exposed to normal temperature (20℃) and peritoneally injected with normal saline as control group; 50 birds were exposed to low temperature (7-9℃) and peritoneally injected normal saline as low temperature group; 50 birds were exposed to low temperature and peritoneally injected with low dose BQ123 as low temperature low dose BQ123 group; 50 birds were exposed to low temperature and peritoneally injected high dose BQ123 as low temperature high dose BQ123 group.

本试验采用高选择性ETA受体拮抗剂BQ123预处理低温肉鸡，探讨ET在低温诱发AS模型的肺血管重塑和肺动脉高压发生发展中的作用。200只16日龄AA肉鸡随机均分为4组：常温(20℃)对照组、低温(7～9℃)对照组、低温低剂量BQ123组和低温高剂量BQ123组。23日龄、30日龄时进行肺动脉压的测定，肺组织取材、石蜡切片，Weigert间苯二酚复红染色。

Following successful modeling, rats of bFGF group were intratracheally injected with 400 U bFGF and rats of VEGF group with 2 μg VEGF, once a week for three times. MSCs group was injected 1 mL suspension of 4×109/L MSCs into tail vein. MSCs+VEGF group was injected MSCs into tail vein and intratracheally injected VEGF (2 ug, three times) at the same time. Model control and normal control groups were intratracheally injected with equal volume of sodium chloride.

成功造模后，碱性成纤维细胞生长因子组气管内注入400 U碱性成纤维细胞生长因子，血管内皮生长因子组气管内注入2 μg血管内皮生长因子，1次/周，共3次；单纯细胞移植组于尾静脉注入4×109 L-1骨髓间充质干细胞悬液1 mL；血管内皮生长因子+细胞移植组气管内注入血管内皮生长因子的同时，尾静脉注入骨髓间充质干细胞；模型对照组、正常对照组给予相同体积的生理盐水。

The mice in experimental group and control group were exposed to 350 cGy radiation produced by 60Co. After 3 h, karyocytes at different concentrations in the fresh human umbilical cord blood were injected into the mice in experimental group A, B, C via their tail veins, and the equal volume of normal sodium was also injected into control group via tail veins. After one month, carbon tetrachloride (CCl4) was injected into experimental group A, B and control group via abdominal cavity, and the equal volume of normal sodium was injected into experimental group C. After two months, immunohistochemistry and reverse transcriptase polymerase chain reaction were used to detect the expressions of human cytokeratin-18 (CK18), cytokeratin-19 (CK19) and albumin in liver tissues of all mice.

采用60Co治疗仪γ射线对实验组和对照组行350cGy的亚致死剂量照射，实验组A、B、C3组照射后3h内经尾静脉分别输入1.0×10^7个/只、2.0×10^7个/只和3.0×l0^7个/只人新鲜脐血有核细胞，对照组经尾静脉注入等体积无菌生理盐水。1个月后对实验A、B组和对照组裸鼠经腹膜腔注射四氯化碳(CCl4)，实验C组注射等体积生理盐水。2个月后采用免疫组化和RT-PCR方法检测裸鼠肝组织人源性CK18、CK19和人源性白蛋白的表达。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。