查询词典 initiation

The summary results are below:1. GUS expression under the driving of the BjCHI1 promoter (-1060/+17) was essentially undetectable in the young seedlings under normal growth conditions. GUS activity was first detected in the stigma of young flowers, peaked in the young siliques, and decreased when the siliques became older. No GUS expression was found in the mature siliques, seeds or root.2. The BjCHI1 promoter (-1060/+17) was inducible by NaCl, PEG, wounding and MeJA treatments. High levels of GUS expression were detected in the transgenic tobacco and Arabidopsis plants after wounding, NaCl, PEG, and MeJA treatment, indicating that the BjCHI1 promoter responses to both biotic and abiotic stresses.3. RT-PCR analysis confirmed that the expression of the BjCHI1 gene in B. juncea was inducible by PEG and NaCl.4. The transcription start site was determined by 5′-RACE, and was located at the 17th nucleotide upstream of the translation initiation codon of the BjCHI1 gene.5. A -805/+17 promoter fragment was enough to response to wounding and MeJA induction, which was proved in transgenic tobacco and Arabidopsis plants. The 397 bp region between -805 and -409 of the BjCHI1 promoter contains a cis-acting element that is essential for the wounding and MeJA inducibility.6. The -695/-620 region was necessary but not sufficient to confer MeJA-responsive expression. A T/G-box locates in -353 play an important role in the expression of the BjCHI1 gene in response to MeJA treatment. The 76 bp region is coupled with the T/G-box to confer full MeJA-inducible transcription of the BjCHI1 gene.

主要结果如下：1、利用转基因拟南芥植株分析表明，正常生长条件下，BjCHI1启动子(-1060/+17)驱动GUS基因主要在花柱中表达，幼嫩的荚也有表达，并随着果荚的成熟而减弱，成熟的果荚、种子和根没有显示GUS活性。2、BjCHI1启动子(-1060/+17)能驱动GUS基因在转基因烟草和拟南芥中响应伤害的诱导，转基因拟南芥的分析还证明BjCHI1启动子也受MeJA、NaCl和PEG的诱导，证明BjCHI1启动子是一个伤害、MeJA、NaCl和PEG等生物和非生物因素诱导启动子。3、RT-PCR进一步证明芥菜中BjCHI1基因也受NaCl和PEG的诱导表达。4、5′-RACE法鉴定了BjCHI1启动子的转录起始位点，位于翻译起始位点ATG上游第17个碱基A.5、转基因烟草和拟南芥分析证明，-805/+17的启动子片段足以响应伤害和MeJA的诱导，-805和-409之间397 bp的启动子片段含有对伤害和MeJA诱导必要的元件。6、本明烟叶片瞬时表达系统分析证明，一段76 bp的序列(-695/-620)对BjCHI1启动子响应MeJA的诱导是必要的，但不足以响应MeJA的诱导，位于-353的T/G-box也参与MeJA的诱导。76 bp的序列(-695/-620)与T/G-box协同起作用，赋予BjCHI1启动子MeJA诱导性。

In addition concentrated QC can also inhibit the growth of skin tumor caused by shope papilloma virus. But neither concentrated QC, nor D77 could inhibit the mouse skin tumors caused by chemical carcinogen and tumor promoter with initiation and promotion protocol.

另外中药QC膏剂还可抑制Sh〓乳头瘤病毒所致的兔乳头瘤；D〓腹腔注射时对S-180腹水瘤也有一定的抑制作用；但无论是中药QC膏剂，或D〓对化学致癌物3-甲基胆蒽与促癌物巴豆油合用诱生的小鼠皮肤瘤无抑制作用。

In ancient Egypt priests and priestesses used scrying as part of their initiation ceremonies.

古埃及的牧师和女祭司将占卜做为他们仪式开始的一部分。

Soul schisms occur in the ascension process when a portion of one's soul is left behind in a particular segment of initiation.

当你在提升的个别阶段中，留下了灵魂的一部份，就发生了提升中的灵魂分裂。

Organization of large-scale chamber blasting is described in the case of Luoguogou, It is emphatically stated that the following points should be noted during technical desing:(1)spacing of charge roons and deter- mination of blasting parameters;(2)design of initiation network;(3) construction plan;(4) safety check and safety precautions.

以罗锅沟大爆破为例，介绍了洞室大爆破的施工组织，并着重问述了在进行技术设计时，应注意以下几点：（1）药室的布置及爆破参数的确定；（2）起爆网路的设计；（3）施工设计；（4）安全校核及安全措施。

Over the next few hours, initiation events occur at other replicons in an ordered manner.

复制子的平均大小是多少在基因组中的个数是多少

In order to elucidate the regulation mechanism of RU5 region to BFV gene expression, BFV3026 provirus DNA was used to construct the plasmids containing different deletion of R region, which were cotransfected with luc report gene locatied behind the IP promoter to BL12 cells, and luciferase activities was assayed, confirming that U5 region could repress the initiation function of LTR as well as IP. The BFV structure genes with different deletion of R region were cloned closely behind to heterogenous CMV promoter, then transfected to 293T cells, RT activity was performed, testifying the R region was required for BFV pol gene expression, and also the function domain was identified within the 100n.t. sequence at the 5′end.

以牛泡沫病毒(Bovine foamy virus， BFV)中国株BFV3026原病毒DNA为材料，构建R区系列缺失质粒，通过对其转染细胞中RT水平及对缺失质粒与luc报告质粒共转染细胞中萤火虫荧光素酶活性的测定，确立U5区对于BFV3026两类启动子LTR和IP均具有负调控作用；同时将带有不同R区的BFV3026结构基因片段克隆于异源启动子CMV之下，通过对其转染细胞293T中RT酶活性的测定，确立R区对于病毒结构基因pol的表达具有一定的调节作用，并将其功能区域初步界定在R区5′端100bp内。

Each of these sequences is important for the binding of rna polymerase and initiation of rna transcriptio ...

本文综述了dna疫苗的意义，特点，作用机理及研制技术，并展望了dna疫苗的应用前景。

Keeping this in mind, a study was undertaken to determine the effect of CA on early growth of mung bean (Phaseolus aureus=Vigna radiata), morphogenetic response in hypocotyl cuttings of mung bean, and changes in the activities of some associated enzymes – proteases, polyphenol oxidases, and peroxidases – and endogenous total phenolics at root initiation (third day; 48 h; appearance of root primordia; RI), root expression (fifth day; 120 h; appearance of roots; RE), and post-expression (seventh day; 168 h; PE) stages of rhizogenesis.

保持这一点，一项研究，以确定影响钙对早期生长绿豆（ phaseolus金黄色葡萄球菌=豇豆拉迪雅塔），形态发生反应，下胚轴插条绿豆，和变化，在活动的一些相关的酶-蛋白酶，多酚氧化酶，过氧化物酶-和内源性总酚的从根本上启动（第三天； 4 8小时；外观根原基；里），根的表达（第五天； 1 20改为H级；外观根；转口），和后表达（第七天； 168改为H级； PE ）的阶段rhizogenesis 。

By means of self-designed test method ,thermal-fatigue resistance and influence factor for surfacing metal of three self-produced flux-cored wires and one solid wire for roller hardfacing were studied deeply.The mechanisms of thermal-fatigue crack initiation and extension were investigated .

通过设计的热疲劳试验方法，对三种自制药芯焊丝和一种国产堆焊轧辊用实心焊丝的耐热疲劳性能及其影响因素进行了深入研究，同时对堆焊金属中热疲劳裂纹形成以及扩展机理做了进一步探讨。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。