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The best optimum conditions of the Agrobacterium mediated genetic transfornation were that leaves explants were immersed in the diluter 10 times Agrobacterium bacilli at OD600 of 0.6 for 8 minutes after being pre-cultured for 2 days,and then co-cultivated for 3 days.Then these explants were transferred to selected and rooting medium which contained 50 mg/L Kanamycin and 500 mg/L Carbenicillin.According to the transformation system, 54 transgenic plants were initially screened,then after PCR analysis and RT-PCR analysis 25 transgenic plants were obtained.

根癌农杆菌介导的芪合酶基因遗传转化的最佳条件为：将预培养2 d的烟草外植体，用稀释10倍的GV3101菌株菌液（OD600=0.6）浸染8 min后，共培养3 d，然后转入含卡那霉素50 mg/L和羧苄青霉素500 mg/L的筛选分化培养基上诱导分化，待抗性芽长到2～3 cm后，转入含Km 50 mg/L和Cb 500 mg/L的筛选生根培养基上进行筛选，在以上最佳遗传转化条件下，初步筛选到54株转化体，经PCR和RT-PCR检测获得25株转化烟草植株。

Both our patients presented with an acute onset of facial diplegia accompanied by other mild symptoms such as diplopia, dysphagia, and neck and limb weakness. The presence of hyperreflexia confused us, and in both patients, cerebral disease was initially suspected. Brain MRI was unremarkable, but electrophysiologic studies indicated peripheral involvement of the facial nerves.

但2例患者进一步的脑部MRI检查均未见异常，电生理检查却均提示周围面神经受累；况且，2例患者的一些临床特点均另我们考虑到格林-巴利综合征的可能：患者1有前驱水样便病史，而患者2的脑脊液则表现出蛋白细胞分离现象。

Initially done as a photo book project, each 'empty bottle' portrait is presented as diptych alongside a blank institutional blue-green page of the same size, in homage to the color used in public municipal space around China.

起初这一作品是做成相册形式的，每一张"空瓶子"的照片旁都有一张同样大小的蓝绿色的空白页面，共同组成一幅两折画面，这也是为了表达摄影师对于在中国公共市政空间里随处可见的绿色的敬意。

A diagnosis chart was made by applying the method of the maximum likelihood discriminatory analysis. Thus the qualitative diagnosis was initially achieved.

从而对中医证候诊断的规范化、定量化和标准化研究进行了尝试，也为其它证候诊断定量化研究奠定了方法学基础。

Initially it considers the process whereby the concept has become discursively fixed into something seemingly homogeneous, and located carefully in relation to both established and emerging debates about, for example, community, design and sustainability.

最初认为，这一概念已成为话语，采取一些看似同质固定的进程，并发展成为新兴的争议，例如，设计位于社区，具有可持续性。

The introduction of such travel technology initially led to disorientation and required a new outlook.

这种旅行科技的引入，最开始导致我们迷失方向，并需要一个新的前景。

It was studied initially that the dispersive stability of TiO2 and antibacterial capability.

并对其分散的稳定性及抗菌性能进行了初步的探讨。

This text is mainly about the analysis of the palindromes of Y chromosome. We mainly used DOTTER program for detailed comparison of two sequences to obtain these results. The main contents are as follows:Chapter one mainly introduces the whole characteristics of human Y chromosome;Chapter two mainly describes the characteristics, functions and recombination modes of the palindromes in Y chromosome;Chapter three mainly analyses the symmetries of 8 palindromes of Y chromosome, we find a mechanism of symmetry breaking in P2 palindrome from a detailed analysis;initially introduces other 7 palindromes.

本文主要借助于DOTTER程序对Y染色体的回文序列进行分析，主要内容如下：第一章主要介绍人类Y染色体的整体特征；第二章主要描述Y染色体中回文序列的特征、功能及重组模式；第三章主要是对Y染色体8个回文序列的对称性分析，主要对P2回文进行了详细分析，提出了P2回文的对称性发生破缺的机理；对其他7个回文进行了初步分析。

This text is mainly about the analysis of the palindromes of Y chromosome. We mainly used DOTTER program for detailed comparison of two sequences to obtain these results. The main contents are as follows:Chapter one mainly introduces the whole characteristics of human Y chromosome;Chapter two mainly describes the characteristics, functions andrecombination modes of the palindromes in Y chromosome;Chapter three mainly analyses the symmetries of 8 palindromes of Y chromosome, we find a mechanism of symmetry breaking in P2 palindrome from a detailed analysis;initially introduces other 7 palindromes.

本文主要借助于DOTTER程序对Y染色体的回文序列进行分析，主要内容如下：第一章主要介绍人类Y染色体的整体特征；第二章主要描述Y染色体中回文序列的特征、功能及重组模式；第三章主要是对Y染色体8个回文序列的对称性分析，主要对P2回文进行了详细分析，提出了P2回文的对称性发生破缺的机理；对其他7个回文进行了初步分析。

Compared to that of two-arm PCL18–DBAS (Figure 1a), the NMR spectrum of the mixture revealed that characteristic methylene proton signals of DBAS, which initially overlapped with signals corresponding to the PCL main chains, shifted downfield to d=4.5–4.7 ppm upon complexation (Figure 1c).

相比于二臂PCL18-DBAS（图1a），此混合物的核磁共振谱揭示，DBAS的特性亚甲基质子信号，他们一开始是与相应于PCL主链的信号重叠的，而在络合作用下则向下面漂移到δ＝4.5～4.7ppm（图1c）。

The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了，排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意，我建议我们在价格上大家各让一半。