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Methods BALB/c mice were anesthetized with ether and infected intracranially with 20 μl 4.5×108 plaque forming units of coxsackievirus B3. CVB3 RNA in mice brain was detected by reversal transcription polymerase chain reaction and the pathological changes of brain tissues were observed by HE staining.

BALB/c纯系小鼠分两组，实验组小鼠颅内直接注射滴度为4.5×108 pfu的柯萨奇病毒B3悬液20 μl，对照组颅内注射生理盐水20 μl，应用逆转录酶聚合酶链反应法测定脑组织中CVB3 RNA含量，HE染色观察脑组织病理学的改变。

The results showed that typical FMD clinical signs produced in cattle inoculated intradermally with pathogenic FMDV after 48 h. The 3ABC antibody produced in these cattle were detected to be suspicious on the 3rd day, 3ABC antibody in the 1/4 cattle were positive on the 5th day, and the 4/4 were positive on the 8th day post virus inoculation; In 10 co-housed cattle (breeded with artificially infected pigs and sheep separately), no 3ABC antibody were detected on the 7th day and percent of positive 3ABC antibody was only 30%(3/10) on the 15th dpi (4/10 had clinical symptom).

实验表明，4头牛在舌面接种感染FMDV 48h后全数发病，第3天即出现可疑的3ABC抗体，第5天检测到阳性的3ABC抗体，至第8天3ABC抗体全部为阳性；10头同居牛（分别与接种感染猪和羊同居）在同居第7天时未检出3ABC抗体，在同居第15天时，3ABC抗体阳性率仅为30%；肌肉接种病毒羊3ABC抗体产生时间与接种病毒牛一致。

Results: Our results indicated that while at the onset of acute HC infection a measurable CMI with effector function was detected in the majority of subjects, after approximately six months less than 10% of chronically infected indiiduals displayed significant CMI compared with 70% of subjects who cleared the irus.

结果：急性丙肝病毒感染初期，大多数样本就可以测量到细胞介导免疫效应子功能。6个月后，样本细胞介导免疫的测量值在病毒清除组（70%）与病毒慢性感染组（10%）间存在明显差异。

The cells infected and noninfected were combined with PLGA at a concentration of 5×106cells/ml to construct tissueengineered bones respectively.They were further implanted into 8 rabbits subcutaneously using PLGA as blank control(8 implants per group). Six weeks after surgery, the implants were evaluated with histological staining and computerized analysis of new bone formation.

方法］PLGA与腺病毒Ad-BMP-7感染后的兔MSCs共培养，电镜观察了解细胞在PLGA上黏附、生长和合成分泌骨基质成分的情况；将转染基因的细胞和未转染基因的细胞分别与PLGA支架复合，构建组织工程化骨并植入体内，通过组织观察新骨形成情况。

How does Vinod Mishra explained this findings, more women could be entering marriage already infected he says. Or they could be getting HIV from nonsexual causes, maybe they receive the injection with the needle that has been used before on someone with HIV.

Vinod Mishra如何解释这种研究结果，很多的妇女在婚前就受感染了，他说，或者他们能够通过非性关系感染HIV，可能他们的感染是通过使用已经携带者使用过的注射针。

Culture tender leaves in culture medium of MS+2,4-D1.5mg/L+30g/L suger+0.7% agar pH5.8 for 20 days in darkness at 25℃, and then subculture to induced Ⅱ-type calli. Use forceps cutting the tissue to nubble with 2mm2, and put the tissue into Agrodacterium tumefaciens LBA4404 liquid supplemented with AS 100mg/L,then, co-culture 3 days, resume 7 days in MS+2,4-D1.5mg/L+30g/L suger+500mg/L cef, take to MS+2,4-D1.5mg/L+30g/L suger+100mg/L cef+10.0mg/L kanamycin culture 20 days in darkness. After that to make it polarize in MS+30g/L suger+100mg/L cef+10.0mg/L km. The percentage of km resistant callus was reached max after infection for 45 min, the average is 29.66%. Simultaneity, tender leave callus are infected 5 min by A. tumefaciens liquid in different negative pressure, and kept on 15 min in Agrodacterium tumefaciens liquid without negative pressure. Then screen out resistant callus and obtain transgenic plants. When the negative pressure is -0.05MP the percentage of km resistant callus was reached max, the average rate is 37.5%.

将心叶接种于MS+2.4-D1.5mg/L+30g/L 蔗糖，琼脂0.7%，pH5.8 培养基中25℃暗培养20d 后继代一次，诱导产生Ⅱ型胚性愈伤组织，用镊子将其夹碎成2mm2大小的小块，置入添加100mg/L AS 的根癌农杆菌LBA4404 菌液中，侵染时间为45min，共培养3 天后，转入MS+2.4-D1.5mg/L+30g/L 蔗糖+500mg/L Cef 培养基中恢复7天，再转入MS+2.4-D1.5mg/L+30g/L 蔗糖+100mg/L Cef+10.0mg/L Km 中，遮光培养20d后，置入其MS+30g/L 蔗糖+100mg/L Cef+10.0mg/L Km 分化，卡那霉素抗性愈伤组织获得率在侵染45min 时达到最大值平均为29.66%；同时以甘蔗心叶愈伤组织材料，通过循环水式真空泵，产生负压，设定不同的负压值，在农杆菌菌液中感染5min 后，在负压条件下继续侵染15min 后，筛选出抗性愈伤组织并获得转化植株，其中在负压为-0.05 时转化率达到最大值，其卡那霉素抗性愈伤组织获得率平均为37.5%。

Methods P.berghei infected BALB/e mice were treated with 0.03μg of IL-12 alone or in combination with 40 U of IL-2 once a day for six consecutive days. Parasitemia was monitored and survival time was calculated.

BALB/c小鼠接种5 105个感染P.berghei的RBC，同时分别给予0.03 μg/d IL-12、40 U/dIL-2或IL-12联合IL-2处理，连续用药6 d，观察小鼠原虫血症变化及存活时间。

All these results above showed that the receptor for bovine rotavirus was a kind of glycoprotein with a complex structure.Sialic acid remnant is infected to be a main part of the receptor site reacting with bovine rotavirus.

实验结果表明牛轮状病毒受体是结构复杂的糖蛋白，唾液酸残基是受体与牛轮状病毒作用位点的组成部分。

There is no need to go into their bizarre activities here in any detail, but we may mention in passing the official's daughter who carried a whip with her to beat ricksha coolies; the division commander who asked an American doctor in Shanghai to kill his son because he had inherited his father's syphilis; the official's wife who loaded dogs onto a plane in place of her fellow-countrymen who were fighting to escape Hongkong after Pearl Harbor; and the general who in the summer of 1948 seduced the eight-year-old daughter of a minor official in Nanking, infected her with gonorrhea and then threatened to arrest the girl's father and his neighbors if they pressed suit against him.

珍珠港事件爆发后，香港的华人争相出逃，一位官太太把她的几条狗塞进飞机座舱，硬挤掉了几个人的机座。某将军在一九四八年夏诱奸了南京一个小公务员的八岁女儿，使她染上了淋病之后，又对女孩的父亲和邻居进行威胁，扬言如果他们胆敢控告他的话，就要把他们全都抓起来。他们之所以能这样为非作歹，就是靠着有这位独裁者在台上。

To study IgG RFs in patients with other autoimmune disease, we have constructed phage display antibody libraries from a hepatitis C virus infected patient with Sjgren's syndrome which is characterized by lymphocytic invasion of exocrine glands resulting in xerostomia and heratoconjunctivitis sicca.

为了了解IgG的类风湿性因子在其他自体免疫疾病上所扮演的角色，我们从C型肝炎病毒感染并发萧格式症候群Sjgren's syndrome 的病人上做了一个噬菌体展现抗体基因库。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。