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Geminiviruses infecting Ageratum conyzoides and Stachytarpheta jamaicensis were identified with molecular data in this paper.5 virus samples collected from Ageratum conyzoides and Stachytarpheta jamaicensis in Hainan were detected with triple antibody sandwich ELISA and PCR, results showed all the virus samples were infected by whitefly-transmitted geminiviruses.
本研究对侵染胜红蓟和假马鞭的双生病毒进行了分子鉴定,并获得如下结果:利用三抗体夹心ELISA及PCR检测的方法对采自海南的胜红蓟和假马鞭的5个病样进行了检测,表明均为粉虱传双生病毒。
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The early stage therapeutic effect on sheep infected artificially with Coenurus cerebralis using Oxfendazole was conducted in order to seek a new drug to cure Coenurus cerebralis of sheep,On the 8 and 15 days after infection, the sheep were treated with Oxfendazole at the dose of 30 mg/kg.bw.
为了寻找一种治疗脑多头蚴早期感染的新药物,应用奥芬哒唑于感染后第8 d和第15 d以30 mg/kg的剂量,对人工感染脑多头蚴的绵羊进行了单剂量和三剂量早期治疗试验。
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Direct avian-human H5N1 virus transmission is the predominant means of human infection, through direct contact with infected birds and virus-contaminated fomites or with fertilizer containing poultry feces.
禽流感病毒经过不断变异后有可能突破种属屏障而传播给哺乳动物及人类,从而进一步在病毒和宿主的多种因素作用下导致宿主发病乃至死亡。
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Rat glioma cell C6 was infected by the deficiency recombinant retrovirus (named C6TK). The sensitivity of C6TK cells to GCV or ACV increased 450 or 10 times than that of C6 cells respectively. Rat C6 glioma model was successfully built, and SD rats inoculated intracerebrally C6TK cells had normal gliomas. The average survival periods of the rats were about 15 days. However, after treated with ACV, the growth of C6TK tumors obviously regressed, and the survival periods extended to 57.8±8.1 days, especially in rats injected with mixed PA317TK and C6 cells or in situ PA317TK cells, which the tumors nearly disappeared after ACV administration and the survival periods extended to over 120 or 71.4±36.1 days respectively, P<0.001.
结果:构建了带有HSV-tk基因的反转录病毒载体GINaTK,应用脂质体转移方法将GINaTK导入反转录病毒包装细胞PA317,成为产病毒细胞PA317TK,用带有HSV-tk基因的复制缺陷型反转录病毒感染C6细胞,命名为C6TK细胞,对GCV和ACV的敏感性分别高于亲本450倍和10倍;成功地建立了大鼠脑胶质瘤模型,并证实转染细胞C6TK的成瘤效应未改变,存活期约为15天;而经ACV治疗后,含有C6TK细胞的肿瘤生长明显被抑制,大鼠生存期延长为57.8±8.07天,尤其是采用PA317TK细胞混和治疗组和原位治疗组,肿瘤基本消失,大鼠生存期显著延长,混和治疗组存活120天以上,原位治疗组存活至71.4±36.1天。t检验,P值均小于0.001。
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Results: GINaTK retroviral vector containing HSV-tk was constructed and transduced into PA317 retrovirus packaging cell by lipofectin (named PA317TK). Rat glioma cell C6 was infected by the deficiency recombinant retrovirus (named C6TK). The sensitivity of C6TK cells to GCV or ACV increased 450 or 10 times than that of C6 cells respectively. Rat C6 glioma model was successfully built, and SD rats inoculated intracerebrally C6TK cells had normal gliomas. The average survival periods of the rats were about 15 days. However, after treated with ACV, the growth of C6TK tumors obviously regressed, and the survival periods extended to 57.8±8.1 days, especially in rats injected with mixed PA317TK and C6 cells or in situ PA317TK cells, which the tumors nearly disappeared after ACV administration and the survival periods extended to over 120 or 71.4±36.1 days respectively, P <0.001.
结果:构建了带有HSV-tk基因的反转录病毒载体GINaTK,应用脂质体转移方法将GINaTK导入反转录病毒包装细胞PA317,成为产病毒细胞PA317TK,用带有HSV-tk基因的复制缺陷型反转录病毒感染C6细胞,命名为C6TK细胞,对GCV和ACV的敏感性分别高于亲本450倍和10倍;成功地建立了大鼠脑胶质瘤模型,并证实转染细胞C6TK的成瘤效应未改变,存活期约为15天;而经ACV治疗后,含有C6TK细胞的肿瘤生长明显被抑制,大鼠生存期延长为57.8±8.07天,尤其是采用PA317TK细胞混和治疗组和原位治疗组,肿瘤基本消失,大鼠生存期显著延长,混和治疗组存活120天以上,原位治疗组存活至71.4±36.1天。t检验,P值均小于0.001。
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Serum samples from cases with schistosomiasis or other helminth infections were tested. Results The positive rate of MPAIA was 96.7%(116/120) with the sera of S. japonicum -infected cases. No cross reaction was observed with sera of trichinellosis, paragonimiasis or cysticercosis cases.
结果 用磁微粒分离酶联免疫法检测日本血吸虫虫卵抗体,阳性检出率为96.7%(116/120),与旋毛虫、并殖吸虫、嚢尾蚴等其他寄生蠕虫抗体无交叉反应现象,检测试剂4 ℃可保存12个月。
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First the subjects were injected with infected lung tissue taken from the dead and then sprayed in the eyes, nose, and mouth with infectious aerosols. If they still failed to succumb, they had their throats swabbed with discharges taken from the sick and dying. If all else failed, they were required to sit open-mouthed while a gravely ill victim was helped to cough into their faces.
首先,从死者身上取下感染的肺组织注射到试验对象的身上,然后用传染性的气雾剂喷在他们的眼睛里、鼻子里和嘴里;要是他们仍没有倒下去,就从病人和临终病人身上直接取来排泄物,抹在他们的咽喉里;要是所有别的办法都告失败,就要求他们张开嘴巴坐着,同时让重病人稍稍坐起身来,朝着他们的脸咳嗽。
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OPO11-964 shows 93 % identity with 1 EST sequence from Cabernet Sauvignon, shows 90% and 87% identity respectively with 2 EST sequences from green grape berries library, shows 83% identity with 1 EST sequence from multiple pathogen-infected sweet orange.
OPO11- 964序列与欧洲葡萄&赤霞珠&的1条EST序列有93%同源性,与&霞多丽&的2条果实不同发育时期获得的EST序列有90%和87%的同源性,与甜橙在多种病原菌侵染后通过cDNA文库获得的1条EST序列有83%的同源性。
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The progress levels of symptoms after pathogen infection showed that interaction of gray leaf spot pathogen and host has differentiation or polymorphism. The progress levels of symptoms is different.The aggressively has polymorphism. The pathogenicity of different isolates in same cultivar has polymorphism, or the host reaction type of same isolate in different cultivars has polymorphism.The host reaction type has differentiation. There are seven types of host reaction in cultivar and inbred after infected by GLS pathogen:Rectangular lesion without chlorotic halo, Rectangular lesion with chlorotic halo,Irregular lesion without chlorotic halo,Irregular lesion with chlorotic halo, Spot lesion with chlorotic halo,Rectangular and irregular lesionand Rectangular and spot lesion. The host reaction type is coalesced lesion when disease severely. Frequency of each reaction types is different.
通过对玉米灰斑病菌侵染后的显症过程的系统观察,可在组织水平上说明该病菌与寄主互作反应存在分化或多样性:玉米灰斑病菌不同菌株侵染后显症过程表现出明显的差异;玉米灰斑病菌在侵袭力上存在多样性,菌株间对同一种品种的致病性表现多样化,或者同一菌株对不同品种侵染引起的寄主反应表现为多样性;病斑反应型的分化,玉米灰斑病菌侵染玉米品种或自交系后,主要有7种病斑反应类型,即RH型、RN型、IRH型(不规则形具褪绿晕圈病斑)、IRN型(不规则形无褪绿晕圈病斑)、SH型、RI型(长矩形与不规则形混合病斑)和RS型(长矩形与斑点形混合病斑),若发病严重,病斑连成片,而且这些反应类型出现的频率也不同。
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To understand the infectivity by porcine endogenous retrovirus with porcine skin fibroblast cell in vitro and in vivo, porcine skin fibroblast cell established by our laboratory were co-cultured with neo/HEK293 cell for the infection of RERV in vitro, and were subcutaneously transplantated to SCID (severe combined immuno-deficiency) mice for the infection of PERV in vivo, laying the foundation for valuation of biologic safety of xenotrans-plantation. The event of neo/HEK293 cells infected by PERV occurred during co-culture of porcine skin fibroblast cells with neo/HEK293 cells, expanding the rang of the infection of porcine endogenous retrovirus. Afterpig cells transplantated subcutaneously in SCID mice, the microchimerism (78.57%) of pig cells occurred widel, and there was phenomena of integration of PERV provirus (85.71%) in several organs or tissues remote from the injected sites, indicating infection of PERV in SCID mice in vivo. yet, there is no evidence of active viral replication in analysis of PERV env RNA of these tissues or organs.
为了解猪皮肤成纤维细胞PERV在体外和体内的感染性,通过建立猪皮肤成纤维细胞系,将所建细胞系与人胚胎肾293细胞体外共培养,并移植于严重联合免疫缺陷鼠皮下进行猪皮肤成纤维细胞PERV的体外和体内感染性实验,结果表明,猪皮肤成纤维细胞与人胚胎肾细胞共培养过程中,猪内源性逆转录病霉感染人胚胎肾细胞,进一步证实和拓宽了猪细胞PERV感染人细胞的范畴;猪皮肤成纤维细胞移植SCID鼠皮下后,导致SCID鼠发生猪细胞微嵌合(78.57%)和PERV在体内感染(85.71%)并且波及远离移植部位的多种组织或器官,但是并未检测出SCID鼠组织中表达PERV env RNA。
- 推荐网络例句
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According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.
曝气设备的动力效率在叶轮转速为120rpm~150rpm时取得最大值,此时氧利用率和充氧能力也具有较高值。
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The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.
稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世,形体几无变化。
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When I was in school, the rabbi explained everythingin the Bible two different ways.
当我上学的时候,老师解释《圣经》用两种不同的方法。