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The results showed that plant height increasing speed was significantly slow at the former and middle stage when K level was above 180 kg/hm2.On the contrary,the height of case-hardened plant improved markedly.When the K level was above 135 kg/hm2,the stem girth and maximal leaf area differed significantly as compared with CK,so did LAI.Yield and product value per unit area took the first place when 180 kg/hm2 potassium was used.Average price and proportion of middle and first grade tobacco leaf of 225 kg/hm2 potassium treatment were the highest as compared with other treatments and CK.Four economic characters of the two treatments differed at obvious significant level as companied with CK.The total sugar and K2O were increased,nicotine, total N,protein and Cl- were decreased with increased K levels.The potassium lacked symptom was alleviated with increased K levels,no symptoms was observed when 180 kg/hm2 potassium was used,the degree of weather infected fleck was decreased when the K levels was increased.

结果表明，在烟株生长前中期，当施钾量达到180 kg/hm2以上时，株高增长显著变慢，而定型株高则显著增高；茎围和最大叶的面积在施用135 kg/hm2以上的处理与CK之间的差异性达到极显著水平，叶面积指数也存在显著差异；单位面积产量和产值以施用180 kg/hm2的处理居首位，中上等烟比例和均价以施用225kg/hm2的处理最高，这两种处理的以上4个经济性状与CK之间存在极显著性差异；烟叶中的总糖和K2O含量随施钾量的增加而上升，烟碱、总氮、蛋白质和C-含量则下降；缺钾症状随施钾量增加而减轻，至施用180kg/hm2时已无缺钾症状；烟叶气候斑的发生程度也随施钾水平的提高而减轻。

Methods Calcein-AM stained H9/HIV-1 ⅢB cells were treated with difierent concentrations of PF in the first,second and third trimesters respectively,which was then mixed with MT2 cells and put under fluorescent microscopy for detection of HIV-1-mediated syncytium formation.MT2 cells were treated with cell-free HIV-1 Ⅲ B,and then washed and cultured with different concentrations of PF in the first,second and third trimesters respectively.Furthcrly,the protecting effect of PFs against HIV-1-infected cells Was determined with MTT,viral replication Was evaluated by measurement of the levels of p24 antigen in culture supernatants with ELISA and the inhibition rate Was calculated.

采用荧光染料Calcien-AM标记的H9/HIV-1 ⅢB分别与早、中、晚孕期不同稀释浓度的PF作用后，与MT2细胞混合培养，荧光显微镜下观察合胞体的形成；用游离的HIV-1 ⅢB感染MT2细胞，并分别与早、中、晚孕期不同稀释浓度的PF作用后，用MTT法检测HW-1感染细胞的存活率，用HIV-1 p24抗原试剂盒检测细胞培养上清中p24抗原含量的变化。

In C. indicus, the infected mechanism correlated with season. The infusoriform larvae of dicyemid mesozoan dispersal from the host to sea water in the autumn and winter, then entering host in the spring and summer. The change of dicyemid life cycle stage in the C. indicus kidney correlates with the sharp decrease of infection intensity. However, the dicyemid infection in Octopus marginatus correlates with body size, not with season.

在C。 indicus之分析中可发现，中生虫之感染与季节有关，有性幼体於秋、冬季自寄主章鱼体内扩散至外界，并於春、夏季大量进入寄主，接著在寄主体内发展，每一次生活史之时期转变，伴随著感染强度之骤降，直至成熟之菱形体时期，大量有性幼体出现肾腔中，至秋季有性幼体随寄主尿液排至外界，下一个生活史之循环接著开始。

Methods To analyze 28 cases with thyroglossal duct cyst and fistula (7 cases of recurrence after operation). The infected cases were treated with antibiotic first before operation, and judged the relationship between thyroglossal duct cyst and hyoid bone exactly through checking and sonography, and then to deal with hyoid bone. First, we should inject methylthioninium chloride in the auditory tube to trace one day before the operation or during the operation, with Sistruk operation, and separate the little branches during operation, handling the focus of infection with radio frequency and microwave or armillary transfixion and so on.

回顾分析28例甲状舌管囊肿病例（含7例术后复发者），术前感染病例先行抗炎治疗，通过查体及B超检查，准确判定甲状舌管囊肿与舌骨关系，处理舌骨；术前一天或术中先注亚甲蓝液入囊管内示踪，行Sistruk术式，仔细分离分支，手指触诊疑为残存病灶处，行环形缝扎或射频、微波等处理。

In the colons of infected mice, IL-4,IL-5 and IL-10 slowly increased with prolongated infection time. Conclusion In the mice infected by schistosome japonicum, the immune response of Th2 related cytokines took place mainly in the liver. The spleen played a role of immune regulation.

感染日本血吸虫病的小鼠，肝脏是Th2相关因子免疫应答的主要器官；脾脏是调节免疫的器官；而结肠Th2相关因子的升高可能是整体反应的一部分。

The recombinant viruses, with the gene of reference RV strain VP2, field strain T114 VP6 and T73 G1-serotype VP7, respectively, co-infected Sf9 cell at a multiplicity of infection of 0.5. Harvested the supernatants of culture when the cells were lysed fully (about 7-10 days), or harvested cells before cell lysates (about 4-5 days after infection) and then treated the cells with lysing solution. The supernatants of and cell lysates were ultracentrifuged twice with 40% sucrose cushion at 93 000g, and the sediment products were harvested with TNC buffer. The purified VLPs samples were separated by SDS-PAGE, and then stained by Coomassie blue and silver nitrate and detected by Western blot to analyze the composition of VP2, VP6 and VP7 and their specificity. The morphologic structure of recombinant VLPs was detected by EM.

含标准株VP2、地方株T114 VP6和地方株T73 G1型VP7蛋白编码基因的重组病毒以0.5 MOI共感染Sf9细胞制备2／6／7病毒样颗粒，待细胞完全裂解后收获培养基上清或感染后5天细胞裂解前收获细胞并用裂解液裂解，培养基上清和细胞裂解液样品两次40％蔗糖垫93000g超速离心，沉淀样品经SDS-PAGE胶分离，用考马氏亮兰和硝酸银染色以及Western blot检测样品中VP2、VP6和VP7蛋白的组成以及特异性，电镜检测重组病毒样颗粒的形态结构。

In order to verify the Fluorescent-Quantitation PCR kit specific for Borna disease virus nucleoprotein, compare molecular beacon with common probe and study its practical application, the sensitivity, distinctness, repeatability and stability were tested on BDV and non-BDV infected cells, as well as non-infected controls. Human and animal samples were also tested by the kit. The lowest quantity of virus RNA detected by the kit was 2.5×101, which corresponded to 1 virus copy.

为评价博尔纳病病毒(Borna disease virus， BDV)核蛋白荧光定量PCR试剂盒的各项指标，比较分子信标探针相对普通探针的优势，并了解其实际检测效果，本课题组使用BDV OL持续感染细胞株、非BDV病毒序列转染的OL细胞、正常的OL细胞，对BDV RT-PCR试剂盒的敏感性、特异性、重复性和稳定性进行评估，同时检测部分临床病人和动物外周血液RNA。

With this in mind, the researchers then reanalysed data from knowingly HIV-positive heterosexual subjects in Uganda and Zambia who had HIV-negative sexual partners. Those with a viral load of one million particles per millilitre were more than ten times more likely to accidentally infect their partners each year than those with a load of one thousand.But when the researchers calculated the transmission potential over the entire predicted asymptomatic period, those with viral loads between ten thousand and a hundred thousand copies per millilitre of blood were the most likely to infect their partners. HIV-positive subjects with viral counts of 50,000 infected roughly twice as many people as those with counts of one million.

伦敦帝国学院流行病学家Christophe Fraser和他的同事对收集于1982年到1993年之间的100多例HIV阳性的男性同性恋者的数据进行分析，并结合分析来自乌干达和赞比亚的已知有HIV阴性性伴侣的HIV阳性异性恋受调查者的资料，他们发现中度病毒负荷而无症状的HIV携带者更易播散HIV，这是因为中度病毒负荷的HIV携带者的无症状期较长，而那些高病毒负荷而无症状的HIV携带者虽然传染性稍强，但其传染的持续时间却很短，并且在未经治疗的HIV携带者个体中中度病毒负荷是最常见的人群。

The NS1 protein is produced in large quantities in infected cells, but it is not packaged in the virion. Since killed vaccines for influenza are primarily made with whole virions, a differential antibody response can be seen between naturally infected and vaccinated animals.

NS1蛋白可以在受病毒感染的细胞中大量产生，但是却不会被包装到病毒颗粒中去；由于灭活禽流感病毒疫苗的成分主要是完整的病毒颗粒，因而免疫接种后机体产生的抗体谱会有别于禽流感病毒感染。

Some patients may have been infected through contact with other infected animals, including a Gambian giant rat and a rabbit.

有些病人可能通过接触其他被感染的动物而感染，包括冈比亚巨田鼠和一种野兔。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。