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A warning signal from the system which indicates high EC or EC ratio of a dairy cow is used to inform the milker to pay attention to the suspected cow. The detection thresholds of EC and ECR to determine whether a quarter or a dairy cow is infected with mastitis correspond with the criterion of SCC = 500×103 cells/ml for quarter foremilk of a dairy cow. The performance of online system for mastitis detection in dairy cows is evaluated with several methods, such as the detection thresholds, running analysis and discrimination analysis.

以分房乳 SCC 500 × 103 cells/ml 作为乳房炎感染与否的门槛值，由牛旁采样分房乳样的检测与分析，探讨影响牛乳 EC 大小的相关因素及其关系，如分房乳中的氯离子浓度、挤乳时间、挤乳前后及乳成份，利用变异数分析探究未感染与感染乳房炎的分房乳之间，其 EC 及 ECR 物理性质，SCC、乳酸去氢酵素及 pH 化学性质及乳脂率、蛋白质及乳糖等乳成份的差异，进而利用统计鉴别分析探讨 EC 相关参数结合乳成份参数对乳房炎检测的准确度。

The results showed as follows: 3mg/LBA in differentiation medium was favorable for regeneration of callus; addition of 3mg/LABA, sucrose substituted with maltose, or sucrose partially substituted with mannitol in subculture media improved the status of callus in a degree and enhanced the differentiation frequency; partial desiccation treatment significantly increased the regeneration frequency of callus; addition of AgNO3 or CuSO4 in differentiation medium contributed to plant regeneration , The two resistant calli and fifteen regenerated plants were obtained when the calli of mature embryo of 7001S were infected with Agrobacterium carrying bar and CPTI gene.

结果表明：3mg/LBA对分化有利；继代培养基中加入3mg/LABA，以麦芽糖代替蔗糖，或以甘露醇代替部分蔗糖在一定程度上可以改善愈伤组织状态，提高分化率；干燥处理能明显提高愈伤组织分化率；分化培养中添加一定浓度的AgNO3和CuSO4有利于愈伤组织分化。借助农杆菌介导bar基因和CPTI基因对水稻进行遗传转化，获得2块抗性愈伤组织和15棵再生苗。

The results showed as follows 3mgLBA in differentiation medium was favorable for regeneration of callus; addition of 3mgLABA, sucrose substituted with maltose, or sucrose partially substituted with mannitol in subculture media improved the status of callus in a degree and enhanced the differentiation frequency; partial desiccation treatment significantly increased the regeneration frequency of callus; addition of AgNO3 or CuSO4 in differentiation medium contributed to plant regeneration , The two resistant calli and fifteen regenerated plants were obtained when the calli of mature embryo of 7001S were infected with Agrobacterium carrying bar and CPTI gene.

结果表明：3mgLBA对分化有利；继代培养基中加入3mgLABA，以麦芽糖代替蔗糖，或以甘露醇代替部分蔗糖在一定程度上可以改善愈伤组织状态，提高分化率；干燥处理能明显提高愈伤组织分化率；分化培养中添加一定浓度的AgNO3和CuSO4有利于愈伤组织分化。借助农杆菌介导bar基因和CPTI基因对水稻进行遗传转化，获得2块抗性愈伤组织和15棵再生苗。

To analyze 12 cases (male 10 and female 2) of double infected with typhoid fever and malaria over the past ten years in our hospital. Results: Of them, 8 cases suffered fioom typhoid fever with tertian malaria, 3 cases of typhoid fever with falciparum nalaria, anid the other one was diagnosed by anti-malaria treatment.

收集我院近十年来的伤寒、疟疾重叠感染患者12例(其中男10例，女2例)结果：12例伤寒重叠感染间日疟8例、恶性疟3例，疟原虫阴性的1例是在确诊伤寒后，按伤寒治疗，高热持续不退，贫血加重，经诊断性抗疟治疗，体温降至正常才诊断为重叠感染的。

Three kinds of method of virus elimination-shoot tip culture, thermotherapy and treated with antiviral chemical-were compared and evaluated with detection of strawberry mottle virus and Strawberry mild yellow edge virus by multiplex RT-PCR in Fragaria ananassa cv. Hokowase infected with viruses.

以感染病毒的草莓品种宝交早生为试材，利用多重RT-PCR同时检测SMoV和SMYEV技术，对茎尖培养、热处理、抗病毒药剂处理3种脱毒方法进行了比较和评价，以期为草莓脱毒苗培育提供重要参考。

Coli Min27 strain with mitomycin C. Twenty-one E. coli strains with various serotypes were infected with FMin27, and plaque formation and lysogenic conversion of them were investigated.

用丝裂霉素对该突变菌株进行诱导，结合抗性标记和PCR方法筛选出一株突变的志贺毒素2型噬菌体，命名为FMin27。

While AcMNPV and BmNPV had no stimulatory effect in nonsensitive cell lines BmNPV hr3 has been demonstrated to increase the transcriptional activity of cislinked hel510 promoter. In this study, Sf-21, Bm-N and Hi-5 cell lines were transfected with pBmhel510luc-hr3down, and then were infected with each of AcMNPV, BmNPV and HyNPV. The results indicated that viral factors could interact with hr3 to significantly copromote the transcriptional level of hel510 promoter.

BmNPV hr3已被证明具有增强子功能，将hr3与hel510启动子顺式连接的质粒pBmhel510luc-hr3down转染Sf-21、Bm-N和Hi-5细胞后，再用上述三种病毒分别感染每一种细胞，hel510启动子的转录活性得到显著提高，表明病毒因子能够识别hr3，二者通过相互作用，共同增强解旋酶基因启动子的转录水平。

Methods: Rats with 20-day age were anesthetized intravenously with pentobarbital sodium and their right conjunctival sac was infected with 50ul of virus suspension (containing about 104 TCID50 HSV-1 viron) after the cornea scarified.

以HSV-1悬液经角膜划痕接种结膜囊内复制大鼠HSV脑炎模型，用Northern杂交与ELISA方法检测实验动物治疗前后脑内NGF、BDNF的mRNA与蛋白含量。

Methods Balb/c mice were immunized subcutaneously three times （0,2,4 weeks） with rSj 32 emulsified with adjuvant. Each mouse was infected with （40±1） curacies of Sj （6 weeks）. After 42 days of infection, the mice were killed and the worm number was counted.

用rSj 32×103加佐剂免疫小鼠3次，分别在0、2、4周进行，第6周每鼠经腹部感染（40±1）条日本血吸虫尾蚴，感染后42 d剖杀，计算减虫率。

METHODS Human IL 6 gene was reconstructed in retrovirus vector and transferred into incasing cells PA317 by lipofectamine mediated method. The clones of the cells transferred with hIL 6 were selected by G418. Targeted NIH3T3 cells were infected with the virus granules secreted from PA317 and also selected by G418. The insertion and expression of hIL 6 gene in NIH3T3 cells were analysed with Southern blot and Northern blot. RESULTS Human IL 6 retrovirus vector (pZIPIL-6) was successfully reconstructed.

利用重组载体构建技术将质粒pUCIL 6 cDNA的目的片段连接于逆转录病毒载体上，并以脂质体介导的方法将重组载体转染包装细胞PA317，以G418筛选克隆细胞，浓缩克隆细胞上清以制备重组病毒液，继之感染NIH3T3细胞后，进行Southern blot和Northern blot分析，检测目的基因在靶细胞的整合与转录水平。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。