查询词典 inducible

Northern analysis revealed that it was inducible by drought and cold stresses as well as ABA application.

Northern杂交结果表明，该基因的表达受干旱、寒冷及ABA的诱导。

Hydroxysteroid dehydrogenase/carbonyl reductase ( 3α-HSD/CR,EC 1.1.1.50 ) from Comamonas testosteroni is an inducible enzyme which catalyzes the oxidation of androsterone with NAD+ to form androstanedione and NADH. 3α-HSD/CR was a member of the SDRs family, whose crystal structure had been solved by X-ray diffraction in 2000. The structure of 3α-HSD/CR is a homodimer which shows a typical pattern of Rossmann-fold structure consisting of βαβ units, and reaveals similarity with other members upon the SDRs family.

中文摘要 3α-Hydroxysteroid dehydrogenase/carbonyl reductase ( 3α-HSD/CR，EC 1.1.1.50 )是从Comamonas testosteroni菌体中分离而来的诱发性酵素，利用NAD+为辅因子，将受质androsterone氧化成androstanedione并伴随NADH的生成而完成催化反应。3α-HSD/CR是属於SDRs家族成员之一，其立体结构已经在2000年利用X-ray晶体绕射所解出。3α-HSD/CR在结构上为一同源双聚体，并和其它家族成员一样具有βαβ二级结构所叠合组成的Rossmann-fold特徵。

Forty-eight hours after transfection, cells were harvested to determine luciferase activity by illuminometer. In the presence of RU486 a 40-fold maximum activation of the luciferase reporter gene was observed in cultured cells, whereas in the absence of RU486, no significant activation was observed. There was a positive correlation between the luciferase activation and RU486 concentration in a definite range. The results showed that the RU486-inducible regulatory vector was successfully constructed, which can be used to regulate the expression level of genes of interest in appropriate time by the inducer RU486. This inducible expression vector provides a platform for the research of gene regulation and gene therapy.

加入诱导剂RU486后，可以诱导表达荧光素酶，并在一定范围内两者呈正比，最高可以实现荧光素酶的40余倍的表达，而没有RU486时，几乎没有报告基因的表达，表明RU486诱导调控载体构建成功，可实现对目的基因的表达时间和表达水平的精确调控，为进一步的基因调控研究和和基因治疗提供了良好的工具。

Inducible and constitutive expression vectors for monocotyledonous plants were constructed and used to transfer into maize calli. Plants were differentiated and regenerated from the positive calli screened with gradient concentration of hygromycin. 4 transformed plants with inducible expression vector and 2 with con

PCR检测获得4株用诱导型表达载体转化的植株和2株用组成型表达载体转化的植株，为进一步研究抗逆相关转录因子基因分eb在单子叶中的表达调控，培育抗逆玉米材料打下基础。

To understand whether the GST can protect plant from the damage of ultra-violet radiation,a UV inducible GST gene was isolated from an Arabidopsis cDNA library.The plant expression vector containing the GST cDNA was constructed and transferred into Arabidopsis thaliana plants by the Agrobacterium-mediated vacuum method.Molecular genetic analyses showed that the overexpression of the UV inducible GST could increase the tolerance of the transgenic plants to UV radiation

为了阐明GST在紫外辐射下是否对植物有保护作用，以紫外强烈诱导表达的GST cDNA为探针，筛选拟南芥cDNA文库，获得了这种GST的全长cDNA；利用此cDNA构建植物表达载体，并通过农杆菌介导法转化拟南芥，使其在拟南芥中得到大量表达；通过对转基因植株的紫外辐射耐性分析，证实了该GST的过量表达可明显增强拟南芥对紫外辐射损伤作用的耐受性。

The lysis efficiency was about 90% for heat-inducible vector or around 30% forUV inducible vectors. This set of autolytic units should be transferable to other vectors.

本体系能够通过简单的物理方法，将具有生物活性的胞内酶释放到胞外的，解决了定向进化的一个瓶颈问题。

Gondii, were cloned by PCR respectively. The PCR products were digested by the corresponding enzymes and ligatd into the intermedial vectors. Finally, the inducible RNAi vector, pBSK-HSP70/5UTR-IntronC-HSP70/3UTR, with the HSP70 gene promotor as a promotor, the intron C sequence ofβ-tubulin gene as intervening sequence, 3UTR sequences of HSP70 gene as transcription stop signals, was constructed successfully, and the results of sequencing were correct. 3The construction of the inherited and inducible RNAi vector system of T. gondii: The fragment of SAG1/5UTR-eGFP-SAG1/3UTR in pBSK-SAG1/GFP vector was cloned into the vector of pBSK-HSP70/5UTR-IntronC-HSP70/3UTR to construct pBSK-GFP-Hairpin vector, then the fragment of GFP-Hairpin in pBSK-GFP-Hairpin vector was cloned into pHANA-0.5 vector.

弓形虫可诱导的反向重复序列RNAi载体的构建：设计引物，通过PCR分别扩增弓形虫HSP70基因5&UTR启动子序列(HSP70／5UTR)、HSP70基因3&UTR序列(HSP70／3UTR)及β-微管蛋白基因内含子C序列，通过酶切连接，构建以弓形虫热休克蛋白HSP70基因启动子进行驱动的，以β-微管蛋白基因内含子C序列作为间隔序列，以HSP70基因3UTR序列作为转录终止信号的反向重复序列RNAi载体pBSK-HSP70／5UTR-IntronC-HSP70／3UTR，序列测定结果正确。

MPGES, an inducible perinuclear enzyme, is preferentially coupled with the inducible COX-2 to promote delayed PGE2 generation.

研究PGES在各种生殖过程中的表达规律及其分子调控有助于阐明PGE_2的代谢及作用机制。

Base on the analysis, organophosphate inducible system has been established by treatment of the swimming seedling and drenching roots with organophosphate solution. This inducible system consists of model plant Arabidopsis thaliana and methamidophos, folimat or methyl parathion which are the representative types of highly toxical OPs. Meanwhile, the responses of the esterase isozymes and the total soluble proteins to OPs in Arabidopsis thaliana have been detected by Native-PAGE and SDS-PAGE.

在此基础上，采用模式植物拟南芥和甲胺磷、氧化乐果和甲基对硫磷三种极具代表性的高毒高残留有机磷农药，通过水培和灌根的方法，建立了有机磷诱导系统，并应用变性与非变性聚丙烯酰胺凝胶电泳技术，研究了拟南芥酯酶同功酶和可溶性总蛋白对有机磷胁迫的响应。

The summary results are below:1. GUS expression under the driving of the BjCHI1 promoter (-1060/+17) was essentially undetectable in the young seedlings under normal growth conditions. GUS activity was first detected in the stigma of young flowers, peaked in the young siliques, and decreased when the siliques became older. No GUS expression was found in the mature siliques, seeds or root.2. The BjCHI1 promoter (-1060/+17) was inducible by NaCl, PEG, wounding and MeJA treatments. High levels of GUS expression were detected in the transgenic tobacco and Arabidopsis plants after wounding, NaCl, PEG, and MeJA treatment, indicating that the BjCHI1 promoter responses to both biotic and abiotic stresses.3. RT-PCR analysis confirmed that the expression of the BjCHI1 gene in B. juncea was inducible by PEG and NaCl.4. The transcription start site was determined by 5′-RACE, and was located at the 17th nucleotide upstream of the translation initiation codon of the BjCHI1 gene.5. A -805/+17 promoter fragment was enough to response to wounding and MeJA induction, which was proved in transgenic tobacco and Arabidopsis plants. The 397 bp region between -805 and -409 of the BjCHI1 promoter contains a cis-acting element that is essential for the wounding and MeJA inducibility.6. The -695/-620 region was necessary but not sufficient to confer MeJA-responsive expression. A T/G-box locates in -353 play an important role in the expression of the BjCHI1 gene in response to MeJA treatment. The 76 bp region is coupled with the T/G-box to confer full MeJA-inducible transcription of the BjCHI1 gene.

主要结果如下：1、利用转基因拟南芥植株分析表明，正常生长条件下，BjCHI1启动子(-1060/+17)驱动GUS基因主要在花柱中表达，幼嫩的荚也有表达，并随着果荚的成熟而减弱，成熟的果荚、种子和根没有显示GUS活性。2、BjCHI1启动子(-1060/+17)能驱动GUS基因在转基因烟草和拟南芥中响应伤害的诱导，转基因拟南芥的分析还证明BjCHI1启动子也受MeJA、NaCl和PEG的诱导，证明BjCHI1启动子是一个伤害、MeJA、NaCl和PEG等生物和非生物因素诱导启动子。3、RT-PCR进一步证明芥菜中BjCHI1基因也受NaCl和PEG的诱导表达。4、5′-RACE法鉴定了BjCHI1启动子的转录起始位点，位于翻译起始位点ATG上游第17个碱基A.5、转基因烟草和拟南芥分析证明，-805/+17的启动子片段足以响应伤害和MeJA的诱导，-805和-409之间397 bp的启动子片段含有对伤害和MeJA诱导必要的元件。6、本明烟叶片瞬时表达系统分析证明，一段76 bp的序列(-695/-620)对BjCHI1启动子响应MeJA的诱导是必要的，但不足以响应MeJA的诱导，位于-353的T/G-box也参与MeJA的诱导。76 bp的序列(-695/-620)与T/G-box协同起作用，赋予BjCHI1启动子MeJA诱导性。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。