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60Mm anterior and 1. 20mm lateral to bregma and 4. 00mm ventral to the cortical surface . 2 days later, ICH was induced by the standard method. At 7d, animals were sacrificed to investigate distribution of EGFP-expressing or BrdU-labeled cells around hemotoma.

根据以上实验结果,在分离、培养、传代获得NSC的基础上,采用pEGFP转染或BrdU标记体外培养NSC后,将细胞移植入前囟前1.60mm,矢状缝向右1.20mm,深4.00mm的细胞增生活跃的前脑脑室下区。2d后诱导脑出血模型,出血后7d处死动物检测EGFP或BrdU阳性细胞在血肿周围的分布情况。

Objective To explore the mechanism of brick tea induced fluorosis in areas of higher sea level.

目的 探讨高海拔地区饮茶型氟中毒的发病机制。

The mechanism of brick tea induced fluorosis in higher sea level area is likely to be complicated.

高海拔饮茶型氟中毒可能存在较为复杂的发病机制。

Objective:With environmental pollution and invasion of the inflammation induced by some materials, bronchia asthma and chronic obstructive pulmonary disease have become the important social public problems in recent years.

近年来,在支气管哮喘和慢性阻塞性肺疾病的治疗药物中,肾上腺皮质激素可对抗炎症或过敏介质,通过对慢性阻塞性肺疾病者的前炎症因子、炎症细胞、氧化应激的影响而发挥良好的疗效。

This study was to compare the protein expression profiles between radiation-induced cancer cells and normal cells. METHODS: Immortalized human bronchia endothelial cell line BEAS-2B was irradiated by γ-ray to prepare malignant BR22P50 cells.

本研究通过建立辐射致癌的细胞模型,应用蛋白质组学技术观察辐射癌变细胞和正常细胞间的差异蛋白表达谱,以期获得辐射致癌相关蛋白。

Coli BL21 and purified. The binding activities of immune sera to three EDs were examined with ELISA and Western blotting analysis.②Asthma model was induced with ovalbumin in mice of vaccine group, control vector group and asthma group. Airway pressure-time index and number of cells in BALF were checked. The quantity of goblet cells in bronchiole and the level of MUC5AC mRNA in lung were investigated by PAS staining and RT-PCR assay.

采用ELISA和Western blotting法检测免疫血清抗体对ED的亲合力。2、对DNA疫苗组小鼠制备OVA致敏哮喘模型,检测气道压力峰值—时间指数、支气管/肺泡灌洗液中炎性细胞数量,PAS特染观察小支气管杯状细胞数量及RT-PCR法检测肺组织中粘蛋白MUC5AC mRNA水平,免疫组化和RT-PCR法检测GM-CSF的蛋白与mRNA水平。

Objective Hyperoxia -induced lung injury is the most common cause of bronchopulmonary dysplasia in neonates.

本研究动态观察高氧暴露对新生鼠肺组织VEGF表达的影响,探讨BPD的发生机制。

This is because it is not only the lifting of bronchospasm, but also so that the contraction of smooth muscle increased induced asthma attack and suppress the respiratory center, so that even more difficult to breathe, leading to respiratory failure.

这是因为它不但能解除支气管痉挛,还可使平滑肌收缩加剧,诱发哮喘的发作,且抑制呼吸中枢,使呼吸更加困难,导致呼吸衰竭。

We also discuss the directed motion of Brownian particle induced by the flashing noise in the periodic potential.

因此我们还分析了周期场中闪烁噪声驱动的布朗粒子的定向输运。

Study on the rapid Propagation Technique of Lycoris. Herb and get follow results: In the period found of axenic clone 0.1%HgCl2 is the best disinfector to deal with the Lycoris" bulb ,as to neat part, such as root, leave, and bulbil, is fit to use 0.1%HgCl2 antisepsis 7min. And found the best effect is the bulb scale with base. Root, leaf and bulb scale without base all were not inducement adventitious buds. Different position of bulb had different culture effect. 3-15 of middle part of bulb can be induced most adventitious buds but inner and outer of least it. Incised the bulb with three types (pieces of eight, pieces of six, piece of four), and found the type of pieces of six is best to Lycoris mass production. L.sprengeri is fit MS+BA5mg/l+NAA0.1mg/l and L.squamigera is fit MS+BA5mg/l+NAA0.5mg/l, but the medium fit to culture L.longituba haven"t be found.In the period of Subculture-Found of mass production, the most multiplication of adventitious buds in MS+BA5mg/l+NAA0.5mg/l during subculture of L.sprengeri, L.squamigera.

石蒜属植物快速繁殖技术研究中,在无菌无性系建立阶段:鳞茎以0.1%HgCl_2消毒10-12分钟效果最好,而叶、根尖、鳞芽等较干净部位选用0.1%HgCl_2消毒7分钟;用三种石蒜属植物的叶片、根尖、鳞芽及带基盘与不带基盘鳞片进行培养,以带基盘鳞片诱导分化效果最理想,鳞芽易培养出芽,但数量有限,而叶片、根尖与不带基盘鳞片均未诱导分化;带基盘鳞片为石蒜属植物快速繁殖最佳外植体,以鳞茎中部3-15层芽诱导率高,较外层稍次之,内部鳞片诱导率最低;选用八等分法、六等分法、四等分法切割鳞茎,六等分法综合效果最好;每外植体带三鳞片培养最为适宜;三种石蒜属植物各自适合的培养基成分不同,换锦花在MS+BA5mg/l+NAA0.1mg/l中培养效果最好,夏水仙为MS+BA5mg/l+NAA0.5mg/l,而白花长筒石蒜在各培养基组合中培养效果均不理想,其适合的培养条件有待于进一步研究。

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