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Chicken IL-15(ChIL-15), Which was discovered in 2001, plays a main role in activating NK cells and CD8+ memory T cells, and may therefore have potential as a vaccine adjuvant.According to published ChIL-15 gene sequence, a pair of primers were designed and synthesized to clone ChIL-15 cDNA from ConA-activated chicken splenocytes by RT-PCR. Recombinant plasmid pUC19ChIL-15 carrying the ChIL-15 gene was constructed. The gene encoding mature ChIL-15 (mChIL-15) was amplified from pUC19ChIL-15 by PCR and cloned into pMD 18-T vector. After digested with Kpn I /Pst I , the mChIL-15 gene was subcloned into prokaryotic expression vector pPRoEX橦Ta and transformed into E.coli DH5a .The transformant was induced by IPTG, and the recombinant ChIL-15(rChIL-15) was expressed as a fusion protein with a histidine hexamer tag at the N-terminal end of the protein. Following expression, the protein was purified by ProBond?Nickel-Chelating Resin. The uninduced and induced protein lysates and the purified protein were separated by SDS-PAGE and transferred onto nitrocellulose membrane to identify rChIL-15 by Western blot. The rChIL-15 was used to immune the guinea pig to obtain rChIL-15 polyclonal antibody.

参考已发表的ChIL-15基因序列，设计合成引物，应用RT-PCR技术从刀豆球蛋白A活化的白来航鸡脾淋巴细胞中克隆ChIL-15 cDNA，将其与SmalⅠ酶切处理的pUC19载体连接，构建重组质粒pUC19ChIL-15；用PCR技术从pUC19ChIL-15中扩增出去信号肽的成熟ChIL-15(mChIL-15)基因，与pMD 18-T载体相连构建重组质粒pMDChIL-15；然后用KpnⅠ／PstⅠ双酶切下mChIL-15基因片段，定向亚克隆到经同样酶切处理的带有6个组氨酸标签的表达载体pP_RoEX~HTa中，构建重组质粒pP_RoChIL-15，对其测序确认读框正确后，转入大肠杆菌DH5α中诱导表达并进行纯化，用重组ChIL-15(rChIL-15)免疫豚鼠，制备多克隆抗体；再用HindⅢ／PstⅠ从质粒pP_RoChIL-15上切下mChIL-15基因，定向亚克隆到经同样酶切处理的杆状病毒转移载体pMelBacB中，经酶切、PCR和序列测定鉴定后，与杆状病毒线形化DNABac-N-Blue~(TM DNA共转染Sf9昆虫细胞，经三轮蚀斑纯化，构建重组杆状病毒rBac-ChIL-15，用该重组病毒感染处于对数生长期的Sf9细胞，按不同的时间收取样品，离心后对其上清和沉淀进行SDS-PAGE和Western blot分析。

Instant electric field-induced domain switching, crack initiation and propagation in a poled BaTiO3 single crystal, as well as sustained field -induced indentation crack propagation and 90°domain switching in an unpoled BaTiO3 single crystal have been investigated in situ using optical microscopy.

金相显微镜原位观察了面内极化的BaTiO3单晶在瞬时电场作用下的畴变和裂纹形核、扩展的关系以及未极化BaTiO3单晶在恒电场下Vickers压痕裂纹扩展和电致畴变的关系。

The research which is the important part of the tunable erbium-doped fiber laser is composed of two main parts: 1. the gain part of the tunable erbium-doped fiber laser, which in fact is an erbium-doped fiber amplifier, its output characteristics are discussed both in theory and experiment, and the differences are also discussed between theory and experiment; 2. the saturated absorber part of the tunable erbium-doped fiber laser, also the part to narrow the line width of the fiber laser. The line-width narrowing mechanism in fiber laser induced by unpumped Er-doped fiber is presented from the aspect of coupling wave equation, and the main factor is considered as the standing wave induced by interference. The line-width is narrower when the standing wave is stronger, and the narrowing effect disappears with the disappearance of the standing wave. In the experiment, when adding a Faraday mirror in the laser cavity to remove the standing wave , the narrowing effect disappears. So the theory is proved by the result of the experiment.

本课题的研究内容是集成光学声光可调谐掺铒光纤激光器的重要组成部分，主要分两部分：1、声光可调谐掺铒光纤激光器中增益部分的研究，相当于对掺铒光纤放大器的研究，理论上对掺铒光纤放大器的输出特性进行分析，实验上实现了掺铒光纤放大器系统，并将实验数据与理论计算数据进行对比，分析了误差产生原因；2、声光可调谐掺铒光纤激光器中饱和吸收体部分的研究，即压窄光纤激光器输出线宽的研究，从耦合波方程出发，理论上解释了未泵浦掺铒光纤压窄光纤激光器线宽的原理，分析了影响该作用的主要因素是未泵浦掺铒光纤中相对传输的光干涉形成的驻波：驻波越强，压窄效果越好，相反当没有驻波时，没有压窄效果，在实验上通过在激光器腔内加入法拉第旋转镜使驻波消失，同时压窄效果消失，从而验证了理论分析的正确性。

MeJA treatment induced vanillic acid and caffeic acid productions; whereas treatment of MeSA and the damage caused by third instar larvae of the maize stalk borer induced the production of only caffeic acid and p－Hydroxybenzoic acid respectively.

三种浓度的MeJA处理均使根际土壤细菌群落相似性指数显著降低，6μL/L的MeJA处理使细菌群落的多样性指数显著降低。

The outbreak was induced by eating fresh or half-fresh pomacea canaliculata,indicating that outbreak of verminosis induced by food can also happen in the northland.

本次疫情属于生吃半生吃福寿螺螺肉而发生的广州管圆线虫病暴发流行，提示食源性寄生虫病在北方也存在暴发可能。

Methods Both in vitro and in vivo models of ventilator-induced lung injury were used. Alveolar epithelial cells were stretched in vitro to mimic the lung injury in VILI. Rats were ventilated at large tidal volume to produce ventilator-induced lung injury in vivo. A total of 23 inflammatory cytokines were screened with micro gene array in stretched alveolar epithelial cells.

方法通过呼吸机诱导肺损伤的体内及体外模型，将分离的肺泡上皮细胞于体外拉长，利用基因微阵列技术分析并筛选出上调的炎性细胞因子，之后给予大鼠呼吸机机械通气，留取血清标本，测量这些细胞因子在血清中是否上调。

Compare the lung injury extent and the important physiological parameters to determine the optimal condition for rabbit ventilator induced lung injury, Choosing another 24 new Zealand rabbit, randomly divided into 4 groups:(1)conventional ventilation+PEEP group;(2)ventilator induced lung injury group: employ the experimental condition determined by the first part experiment;(3)VILI+HO-1 inducer hemin group:pre-treated with HO-1 inducer, the ventilation method is the same with VILI group;(4)VILI+HO-1 inhibitor Zn-PP group(Zn-PP group):pre-treated with HO-1 inhibitor, the ventilation condition is the same with VILI group.

第二部分24只家兔随机分为4组：(1)常规潮气量+PEEP组；(2)呼吸机所致肺损伤组：使用实验第一部分确定的最适条件；(3)呼吸机所致肺损伤+HO-1诱导剂干预组：预先给予氯铁血红素，通气条件同VILI组；(4)呼吸机所致肺损伤+HO-1阻断剂干预组：预先给予锌原卟啉，通气条件同VILI组。

The intensity ratio of TO and LO inMCT was observed to be different. Such difference was explained in terms of the different Ramangeometry arrangement.〓. The laser-induced micro-photoluminescence in the range of 1000～5000〓(1.34eV～1.83eV) was found for the first time in LPE MCT epilayer. The center of photoluminescence wasat 2750〓 or 1.62eV and the FWHM of luminescence was 2000〓 or 0.25eV. We assume thatthe photoluminescence is due to recombination of electron from an anion vacancy resonance levelto the top of valance. In addition, new Raman shift was observed at 750〓 in LPE MCTepitaxial film.〓. The laser-induced micro-photoluminescence with quasi-periodic structure was observed forthe first time at room temperature in one of MOVPE MCT epitaxial film samples. The range offluorescence was from 1.46eV to 2.21eV, i.e., 1.73eV above the conduction band edge.

2首次在LPE生长的碲镉汞外延薄膜的显微Raman谱中，在1000～5000〓范围发现了激光激发显微荧光，该荧光的发光范围换算为电子伏特标度为1.34eV～1.83eV，荧光的发光中心大约位于2750〓，即1.62eV，发光的半峰高宽约为2000〓或0.25eV；指出该显微荧光来源于碲镉汞薄膜中的阴性离子空位共振能级的激光激发发光；观察到了碲镉汞外延薄膜中一个新的Raman散射峰，位于750〓位置； 3首次在一块用MOVPE方法生长的〓Te外延薄膜的显微Raman谱中，发现了1.46eV至2.21eV范围并伴随有周期结构的显微荧光峰，该发光峰对应的能带中心位于〓Te材料导带底上方1.73eV，通过研究得出样品在1.46eV至2.21eV范围的显微荧光峰是由于改进 MOCVD 生长工艺，提高了碲镉汞外延薄膜的结构质量所致；通过分析指出该显微荧光来源于外延层中的阴性离子空位的共振能级发光。

The first-order Raman Scattering due to the lattice mismatch-induced strain and the oxygen vacancy-induced micropolar regions were observed in SrTiO〓 thin film.

极化TO2声子显示了很强的Fano型不对称性，而TO3和TO4声子则呈现出高度对称性，TO2声子的Fano效应证实了SrTiO〓薄膜中存在微极化区。

According to the distribution of time resolved relative intensity of continuum emission of the plasma induced by laser ablating Al in Ar,we have briefly discussed the mechanism of the continuum radiation of the plasma induced by laser ablating Aluminum.

根据连续辐射强度的时间分布，简要讨论了激光诱导等离子体连续辐射的产生机理。根据连续辐射强度的波长分布，提出了原子对激光诱导等离子体连续辐射共振吸收理论。

And discuss some key point of integrated management system design, include hazard identification and risk assessment and risk control in the occupational health and safety management, the measure of satisfaction target of customer, the flawlessness of law system, the integration of the integrated management system and enterprise standardization system, unify lead institution and work orgnization, integreted internal audit and integrated management review.

同时，论述了一体化管理体系构建中的几个主要问题，包括职业健康安全管理中的危险源辨识、风险评价和风险控制、顾客满意度的科学监测、法律、法规体系的完善、一体化管理体系与企业标准化体系的有机结合、统一的领导机构和工作机构、一体化的内部审核和管理评审。

Deepwater Taranaki is investigated for its petroleum potential, using all available seismic data tied to shallow-water wells.

运用与浅水井相关的现有全部震波探测数据考察了深海区塔拉纳基盆地的油藏前景。