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Results The mortality rate of mice in 80 mg/kg, day cyclophosphamide group was 16.7%, and T level [ at 30th day :( 1.38 ± 0.31 );45th day:( 1.15 ± 0.26 ) ] and T/LH ratio [ at 30th day:(0.163 ± 0.014); 45th day:(0.127 ± 0.023 ) ] were significantly decreased (all P<0.05) at 30th day after induction;The concentration of MDA [at 15th day:(2.70 ± 0.41);30th day:(2.710.36);45th day:(2.67 ±0.43) ] was maintained at a high level (all P<0.05) during the 45 days ; Number of Leydig's cells [ at 15th day:(9.65 ± 0.75 ); 30th day:( 14.05 ± 0.67 ); 45th day:(8.49 ± 072)] and layers of spermatogenetic epithelia [ at 15th day:(4.75 ± 0.82);30th day:(3.60 ± 0.49);45th day:(3.74 ± 0.43 ) ] were significantly decreased ( all P < 0.01 ) and stabilized in a low level. The induced model was stable and the mortality rate was acceptable. In the 60 mg/kg, day cyelophosphamide group, the T level and T/LH ratio had no significant change (P > 0.05 ), and the concentration of MDA ,number of Leydig' s cell and layers of spermatogenetic epithelia recovered at 30th day after induction. The induced model was unstable.
结果 剂量每日为80 mg/kg体重小鼠成模后死亡率为16.7%,血清T[30 d:(1.38±0.31);45 d:(1.15±0.26)]及T/LH比值[30 d:(0.163±0.014);45 d:(0.127±0.023)]于诱导后第30天出现显著下降(P均<0.05),而诱导后睾丸组织内MDA含量[15 d:(2.70±0.41);30 d(2.71±0.36);45 d:(2.67±0.43)]维持高水平(P均<0.05),生精上皮层次[15 d:(4.75±0.82);30 d:(3.60±0.49);45 d:(3.74±0.43)]和间质细胞[15 d:(9.65±0.75);30 d:(14.05±0.67);45 d:(8.49±0.72)]均显著减少(P均<0.01)并稳定于低水平,模型稳定,死亡率适当;每日60mg/kg体重组小鼠血清T及T/LH比值于不同时段并未出现明显变化(P>0.05),且睾丸组织内MDA含量、生精上皮层次和间质细胞计数在30 d后有所恢复,模型不稳定;每日100 rag/ks体重组死亡率为30.0%,死亡率过高。
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The calculated results indicate that a bifurcated A. wave can be formed when the shock passes through the flame, and pre-induced shock transmission in the flame leads to the formation of Mach stem, followed by spheral shock wave. The Rayleigh-Taylor and Kelvin-Helmholtz instabilities occurred in the flame induced by shock wave lead to the flame distortion and breakup.
计算结果表明,激波掠过火团时,分叉形成λ波;前引激波在火团内透射并在轴线处碰撞形成Mach杆,进而逐渐发展为球形激波;火焰在激波作用下先后出现Richtmyer-Meshkov不稳定和Helmholtz不稳定,火焰逐渐变形以致破碎;燃烧速率因此剧增。
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Stress reduces neurogenesis in the dentate gyrus subgranular zone, but it is unknown if stress-induced changes in neurogenesis contribute to individual vulnerability. Using a chronic social defeat stress model, we explored whether the susceptibility to stress-induced social avoidance was related to changes in SGZ proliferation and neurogenesis.
长期压力已知会引起一些精神疾病,可是尚不清楚一些人比另外一些人更容易产生这些症状的神经器官基矗Bmelia Eisch及其同事研究了大脑的海马区——对进修和记忆具有重要性的区域——并探索了神经器官形成对受压力影响严重的大脑功能的作用。
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The most distinctive substructural feature of the stress-induced martensite after deformation at the characteristic temperature and in the critical strain range is the presence of the (001)〓 twins. Based on the analysis of the stability and shape memory effect of various kinds of martensite, the concept of stability of deformed stress-induced martensite was proposed and explained by the mechanism for the formation of the (001)〓 twins.
在综合分析各种马氏体的稳定性和记忆效应的基础上,提出了形变应力诱发马氏体稳定性的概念,并以其(001)〓孪晶亚结构的形成机制给以较为圆满的解释。
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Microinjection of glucocorticoid or mineralocorticoid (40 or 10 ng/0.1 ul/site) into the rVLM bilaterally caused an increase in BP, HR and the pressor response induced by the dPAG stimulation in the normotensive rat, respectively. Dose-dependence of both drugs was observed on the above effects in three directions. Nevertheless, there was no effect of both drugs on the tachycardiac response induced by the dPAG stimulation.
二、分别微量注射糖、盐皮质激素(40或10ng/0.1μl/site)至双侧rVLM,可引起正常血压大鼠的BP升高,HR加快,以及电刺激中脑中央周围灰质背侧区诱发的防御性升压反应增强,并均呈现出剂量-效应依赖性关系,而对电刺激dPAG诱发的加快心率反应无明显改变作用。
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After microinjection of the antagonists of glucocorticoid RU 38486 or mineralocorticoid spironolactone (40 ng/0.1 ul/site, respectively) into the rVLM bilaterally, a decrease in BP, HR and the pressor response induced by the dPAG stimulation was observed in the normotensive rat, and there was no change on the tachycardiac response induced by the dPAG stimulation.
分别微量注射糖、盐皮质激素的拮抗剂RU 38486(40ng/0.1μl/site)或螺旋内酯(40ng/0.1μl/site)至双侧rVLM,则引起正常血压大鼠的BP下降、HR减慢,以及电刺激dPAG诱发的防御性升压反应减弱,对电刺激dPAG诱发的加快心率反应无明显改变作用。
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When the steel changed in form between Ms and Md,its tensibility enhanced considerably due to s train-induced martensite transformations and transformation-induced plasticity of retained austenite.
该钢在Ms-Md温度之间形变时,应变诱导残留奥氏体向马氏体转变,使结构钢也能产生相变诱发塑性[3]。
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PTX at tenuates the ALI induced by LPS or sepsis in rat. The therapeutic effects of PTX include:improve oxygenation, at tenuate pulmonary edema formation, enhance animal survival in sepsis-induced ALI. The mechanisms may related with the effects of PTX inhibiting the chemotaxis, migration, activation of inflammatory leukocytes, diminishing the production of active oxygen, and inhibiting inflammatory cytokine release partly.
4PTX对大鼠内毒素性ALI及脓毒症性ALI均有一定的防治作用,主要表现在改善动脉血氧合,减轻肺水肿形成,提高脓毒症性ALI大鼠的存活率等方面,其机理可能与抑制白细胞趋化与激活,减少肺内白细胞渗出及活性氧产生,一定程度上抑制炎性细胞因子生成等有关。
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NodD binds to and bends target promoters through anchoring two tandem and individual specific DNA sites. NodD functions as a tetramer, which has a V-shaped main body. Tetrameric NodD is to change its own conformation rather than its oligomeric forms in response to small signal molecules. The specific interaction between each NodD DNA-binding domain and each specific DNA site does not alter itself in spite of naringenin induction, and the induced conformational change is transferred from protein to DNA. Only the DNA conformation incited by induced NodD is competent for RNA polymerase to form the transcriptional open complex. It cannot be excluded that NodD may have protein-to-protein contacts with RNA polymerase, and that the NodD conformational change may also directly contribute to the transcriptional open complex formation. However, the NodD conformational change itself cannot serve as the determinant of the transcriptional molecular switch.
通过研究,我们提出了初步的NodD操纵子激活模型:第一,四聚体是NodD蛋白的功能单位,它通过铆钉两个串联的相对独立的DNA靶位点结合被诱导的启动子;第二,小分子配基的结合是改变NodD四聚体的构象而不是引发不同形式的寡聚体,在我们的模型中,NodD四聚体缩小其V形主体的弯折角,进而缩短其DNA结合功能域的间距;第三,小分子信号的诱导并没有改变NodD的DNA结合域和其DNA靶位点的相互作用,NodD的构象改变由蛋白质经其双铆钉位点传递给DNA;第四,只有诱导状态的NodD激发的DNA构象才能有效地使RNA聚合酶形成转录开放复合物;第五,不排除NodD与RNA聚合酶可能有直接的相互接触位点,不排除NodD构象的改变可能直接有利于RNA聚合酶形成转录开放复合物,但是我们认为NodD构象改变本身不是充当转录激活开关的决定因素。
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Part II The experiment study on anti-tumour effect of dendritic cells derived from peripheral blood monocyte of patients with oral cancer after pulsed by tumour antigen in different ways. Collected 50ml peripheral blood from each patients and volunteers to induced DC in vitro. We parted the test group and control group into three subgroups respectively, untreated group A, freezed and thawn group B, RNA transfected group C. To group A, after induced DCs for five days , we added 200U/mlTNF-α in each hole and cultured on. To group B, we added protein antigen (DC/ tumor cells = 1 : 1 ,each hole) for 4h at 5d, and then added 200U/mlTNF-α in each hole and cultured on. To group C, transfected immature DC with tumor cell RNA(DC:RNA=1×105/ml: 5ug)at 5d, operated completely with the specification of invitrogen DMRIE-C reagents. After transfection we replaced transfection agent with complete medium, and then added 200U/mlTNF-α in each hole and cultured on.
第二部分口腔癌患者外周血来源的树突状细胞体外负载抗原后抑瘤作用的实验研究无菌采集20例口腔癌患者和20例健康志愿者外周血50ml,分离单个核细胞体外诱导培养DC,并将实验组和对照组细胞各分为三组,A组为未处理组,B组为冻融组,C组为RNA转染组。A组DC在培养5d后,每孔加入TNF-α200U/ml;B组DC培养第5d后,每孔中加入癌细胞蛋白抗原,使DC/肿瘤细胞=1:1,继续培养4h后,每孔加入TNF-α200U/ml;C组将培养至第5d的未成熟DC用肿瘤细胞RNA进行转染(DC:RNA=1×105/ml: 5ug,按照invitrogen DMRIE-C试剂说明书进行操作),转染结束后用完全培基取代转染剂,每孔加入TNF-α200U/ml。
- 推荐网络例句
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And discuss some key point of integrated management system design, include hazard identification and risk assessment and risk control in the occupational health and safety management, the measure of satisfaction target of customer, the flawlessness of law system, the integration of the integrated management system and enterprise standardization system, unify lead institution and work orgnization, integreted internal audit and integrated management review.
同时,论述了一体化管理体系构建中的几个主要问题,包括职业健康安全管理中的危险源辨识、风险评价和风险控制、顾客满意度的科学监测、法律、法规体系的完善、一体化管理体系与企业标准化体系的有机结合、统一的领导机构和工作机构、一体化的内部审核和管理评审。
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Deepwater Taranaki is investigated for its petroleum potential, using all available seismic data tied to shallow-water wells.
运用与浅水井相关的现有全部震波探测数据考察了深海区塔拉纳基盆地的油藏前景。
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If you're huffing and puffing at any point during the run, slow down and make sure you can take deep breathes.
如果你任何时候在跑步过程中气喘如牛,降低速度并且确保你能够深呼吸。