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We may well explain why ricin-induced apoptosis showed no typical DNA ladder. The presence of laddering in DNA gels implies an apoptosis process, but its absence does not rule out the apoptosis. The ricin 0.05μmol〓 had no effect on the 〓 phase of cell cycle, but induced 〓 arrest. The ricin may affect the balance of synthesis and destruction of proteins that controlling the cell cycle.

检测到DNA梯度表明细胞发生了细胞凋亡,未检测到DNA梯度不能否认凋亡的存在。0.05μmol.L〓的蓖麻毒素对HeLa细胞周期的G〓/G〓期无影响,而诱导了G〓/M期阻滞,蓖麻毒素可能引起一些控制细胞周期蛋白的合成与降解发生紊乱。

The effect of F 1C on joint swelling and the immune respones of AA rats were evaluated by lymphocytotic reaction, flow cytometry, and ELISA. Results F 1C by ig administration to AA rats decreased the primary joint swelling of AA rats, stronger than TW and weaker than CsA, promoted the decreased splenocyte proliferation induced by Con A, and inhibited the augmented thymocyte proliferation induced by Con A in AA rats. The evaluation in vitro showed the F 1C inhibited the augmented thymocyte proliferation from AA rats and promoted the deficient IgG produced by splenocyte from AA rats.

结果 ig给予F1C(30mg/kg)对AA大鼠原发性足肿胀具有明显的治疗作用,该作用较TW强,较CsA弱;F-1C对AA大鼠低下的脾细胞增殖反应具有改善作用;对亢进的胸腺细胞增殖反应具有抑制作用。F-1C体外对AA大鼠低下的脾细胞产生IgG水平具有明显的促进作用,并能抑制亢进的胸腺细胞增殖反应。

IM-induced autophagy did not involve c-Abl or Bcl-2 activity but was associated with ER stress and was suppressed by depletion of intracellular Ca2+, suggesting it is mechanistically nonoverlapping with IM-induced apoptosis.

IM诱导的自噬与c-Abl 或 Bcl-2活性无关,但与内质网应激相关并受细胞内Ca2+缺失抑制,提示其与IM诱导的细胞凋亡机制不同。

Bone marrow-derived mesenchymal stem cells are capable of chondrogenesis, making them a possible source of cells for injectable cartilage tissue engineering. There exist different ideas on the ability of mesenchymal stem cells's chondrogenesis in monolayer culture. Because of this, the effect of adult rabbit's bone marrow-derived mesenchymal stem cells chondrogenesis in monolayer culture was studied. The mesenchymal stem cells was isolated from adult rabbit's bone marrow and monolayer cultured. TGF-β1, Vit-C and Dexamethasone were used. Immunohistochemistry analyses and histological staining of H-E, Methylaniline blue and Alcian blue were performed to identify the expression of collagen type Ⅱ and cartilage associated matrix. The results showed that the induced cells expressed and produced collagen type Ⅱ and cartilage associated matrix. This suggests that the differentiation of adult rabbit's marrow-derived mesenchymal stem cells into chondrocyte in monolayer culture is feasible and may be induced by TGF-β1, Vit-C and Dexamethasone.

骨髓基质干细胞的软骨分化潜能使其可能成为可注射组织工程化软骨研究的种子细胞,为探讨体外培养的骨髓基质干细胞在平面诱导条件下软骨分化的可行性,我们进行下列实验:获取并体外平面培养成体兔骨髓基质干细胞,应用TGF-β〓、Vit-C和地塞米松对其软骨分化诱导,诱导后的骨髓基质干细胞行细胞爬片组织学和Ⅱ型胶原免疫组化,结果证实,诱导后骨髓基质干细胞可分泌Ⅱ型胶原,组织学染色可见类似于软骨细胞,由此证明体外培养的骨髓基质干细胞在平面诱导条件下可以软骨分化,其软骨诱导因子为TGF-β〓、Vit-C和地塞米松。

High dose copper induced decrease ofⅠ,Ⅱ,Ⅳ-2 content and increase ofⅢof spectrin. High dose copper can also decrease SA, PE content and increase SM, PS content.These showed that high dose can induce injury of erythrocyte by changing elementary structure and moity of membrane protein and membrane phospholipid, which may be one of the mechanisms of goat erythrocyte damage induced by high dose copper. 2. High copper caused the decrease of SOD and GSH-PX activity and the increase of MDA content.

高铜能引起红细胞膜收缩蛋白Ⅰ、Ⅱ及区带Ⅳ-2蛋白含量均显著降低,而区带Ⅲ蛋白含量明显增高,红细胞膜唾液酸含量降低,磷脂酰乙醇胺含量降低,神经鞘磷脂和磷脂酰丝氨酸含量升高,表明高铜能够改变红细胞膜蛋白和磷脂等基本结构和组成成分,这可能是高铜致羊红细胞损伤的机制之一。

No callus could be induced from leaf or petiole explants of varieties F30 and Ⅱ cultured on MS medium if supplemented alone with 6-bezyladenine (6-BA) or thidiazuron, or kinetin. However, the calli were induced on MS medium containing 2.0 mg L-1 6-BA and 0.5 mg L-1 NAA (α-naphthaleneacetic acid), and the calli derived from petioles of variety Ⅱ could produce redifferentiated shoots, but of variety F30 could not. The later could only produce shoots directly from the base of the petioles.

将品种F30和Ⅱ的叶片和带叶叶柄接种到含不同浓度单一细胞分裂素(6-BA、TDZ、KT)的MS培养基上培养,均未能诱导出愈伤组织,而在含2.0mgL-1 6-BA+0.5mgL-1 NAA的培养基上可以诱导出愈伤组织,且由品种Ⅱ叶柄诱导产生的愈伤组织可再分化出芽,品种F30诱导的愈伤组织却不能分化,但在其叶柄基部可直接出芽。

The aim of this brief review is to discuss the safe contrast administration in radiological examination process, including iodinated contrast medium induced extravasations, allergy, and nephrotoxicity via intravenous route; gadolinium induced nephrogenic systemic fibrosis, attention information on other contrast medium or medications.

本文将讨论放射线检查之用药安全流程,包括注射含碘对比剂造成渗漏、过敏或对比剂造成的肾毒性;注射含钆类对比剂发生肾因性全身纤维硬化症、其他对比剂或药物的注意事项。

Chapterl Establishment of the chronic and pressure-induced SD rat models for acoustic neurinomas Object To establish the chronic and pressure-induced SD rat models of acoustic neurinomas for the purpose of further experimental research on acoustic neurinoma related electrophysiology, function and pathomorphology of facial nerves and cochlear nerves.

第一章 SD大鼠听神经瘤慢性压力模型的建立目的建立SD大鼠听神经瘤慢性压力模型,为听神经瘤所致面、听神经电生理、功能及病理学改变的研究奠定基础。

It is therefore concluded that FA can directly activate a subpopulation of primary nociceptor cells and the FA-induced AP discharges are likely to contribute mainly to phase 1, but not phase 2 of the formalin-induced nociception.

以上结果提示,甲醛溶液选择性地激活一个亚群的初级传入伤害性感受器细胞,甲醛激活的动作电位的发放主要参与福尔马林诱发的第一相伤害性感受的产生。

The above results showed that physiological AAI pacing maintained a normal EAS and VAS, and improved or enhanced VSS; while nonphysiological VVI pacing and physiological DDD pacing induced the decrease in VSS due to an abnormal EAS and VAS. Furthermore, with increasing the pacing rate, VSS was markedly decreased.The quantitative comparison of parameters of left ventricular systolic function obtained by computerized ERNA showed that AAI pacing induced the significant increases in ventricular ejection rate and stroke volume with concomitant shortening of ejection time.

应用计算机-ERNA相位分析技术,对13例生理性AAI、13例非生理性VVI及6例生理性DDD患者,分别进行等相位电影,相位图,相位直方图和VSS参数的静动态观察和定量比较,结果显示:AAI起搏可维持正常EAS和VAS,心室相位均匀一致,并且VSS参数-相角程,半高宽和峰相位标准差均较起搏前分别降低,提示起搏后VSS有所改善或增强。

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However, as the name(read-only memory)implies, CD disks cannot be written onorchanged in any way.

然而,正如其名字所指出的那样,CD盘不能写,也不能用任何方式改变其内容。

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