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To investigate the effect of p38MAPK signal conducting pathway on livercancinoma cell's malignant phenotype induced by VEGF,we take theexperiment with cell growth test,scanning microscope and laser scanningconfocal microscope so that observe effect of p38MAPK signal conductingpathway on liver cancinoma cell growth,pseudopodium formation andframework of cytoskeleton induced by VEGF.results indicate that the cellbecame ellipse and there were more and thick pseudopodium in the cell'ssurface after being treated by VEGF,and destroyed framework ofcytoskeleton,which can be blocked by pretreated with a special inhibitor ofp38MAPK SB203580 so that VEGF promote metastasis by enhancing livercell migration and movement via p38MAPK signal conducting pathway,butVEGF promote cell growth without p38MAPK signal conducting pathway.

为进一步探讨VEGF通过p38信号传导通路诱导肝癌细胞转移时，对肝癌细胞恶性表型的影响，采用细胞增殖实验、扫描电子显微镜、激光扫描共聚焦显微镜观察VEGF对肝癌细胞增殖作用、细胞伪足形成、细胞骨架微丝结构的影响以及p38MAPK信号通路调控作用。结果显示，VEGF能够不通过p38信号通路促进肝癌细胞增殖；VEGF诱导肝癌细胞丝状伪足增多、增长，使细胞骨架微丝结构破坏、甚至消失。阻断p38MAPK信号通路，可以抑制VEGF诱导的肝癌细胞伪足形成，细胞骨架丝状结构呈束状，排列较规整。上述结果表明，VEGF可以通过p38信号传导通路诱导肝癌细胞伪足增多、增长，促使细胞骨架微丝结构破坏，使肝癌细胞迁移、运动能力增加，促进肿瘤转移。VEGF并不通过p38信号通路诱导肝癌细胞增殖作用。

The pyrexia may have negative influence on prognosis for patients with acute brain stroke. The treatment for infection and danger factors of infection is important for patients with acute brain stroke. The early identification for infection-induced pyrexia and uninfection-induced pyrexia is of great clinical significance.

急性脑卒中后发热影响患者的预后，感染与易感因素防治是急性脑卒中综合治疗的重点，感染性发热与非感染性发热的鉴别和深入研究具有重要临床意义。

RESULTS:(1)NO production and iNOS mRNA expression induced by LPS and TNF α was blocked by pyrrolidine dithiocarbamate or N-acetylcysteine.(2)LPS and TNF α triggered the activation and translocation of NF κ B, and PDTC or NAC inhibited the activation of NF κ B induced by LPS and TNF α.

结果：抗氧化剂可阻断内皮细胞培养体系LPS和TNFα诱导的NO生成及iNOS mRNA的表达；LPS和TNFα可诱导大鼠肺微血管内皮细胞NFκB的激活并可被抗氧化剂阻断。

In fever model of rats induced by carrageenin, D-KP(2.5、5、10 mg.kg-1) could significantly decrease the high temperature. And in rabbit fever model induced by typhoid vaccine, D-KP(1.25、2.5、5 mg.kg-1) could also decrease the abnormal body temperature.

结果 D-KP（5、10、20 mg.kg-1）能明显延长小鼠热板和热辐射甩尾的痛阈时间；D-KP（2.5、5、10 mg.kg-1）对角叉菜胶诱发大鼠的体温升高、D-KP（1.25、2.5、5 mg.kg-1）对伤寒疫苗诱发兔的体温升高均有明显的降温作用。

The techniques of optical beam induced current have found wide-spread applications in characterizing many semiconductor and optoelectronic devices. A two-photon confocal microscope is adapted for investigating the dynamics of light emitting devices through the contrast mechanisms of two-photon DC and radio frequency optical beam induced current.

摘要 光致电流的技术至今已经广泛的被应用在检测半导体特性以及光学元件上，共焦显微镜搭配双光子激发技术相当适合用来检测LED元件，与单光子激发相较之下，利用脉冲雷射双光子激发术可以使用光子能量小於能隙的长波长光源来完成激发，并且具有高度的空间解析度。

In order to investigate the excitation mechanism and response characteristics of wind-rain induced vibration of stay cable,an instrumentation system of wind-rain induced vibration measurement,which consists of anemometer,rain gauge and accelerometer,was installed on the Dongting Lake Bridge,Yueyang,China.

为了研究斜拉桥拉索风雨振的机理及响应特征，在岳阳洞庭湖大桥建立了由风速仪、雨量计、加速度传感器组成的风雨振现场观测系统，观测到了多次拉索风雨振现象；得到了发生拉索风雨振时风速、风向及雨量的基本条件，分析了风雨振时拉索振动的幅值、主振频率、模态参与特性及空气动力负阻尼比等响应特征。

The blank group, the pure chondrogenic inductor group and the group of the G ranula of P enetrating Bone and Removing Pain mixed with chondrogenic inductor. We adopted pro-culture solution, pure chondrogenic induced culture solution （ TGF-β310ug/L , Dex10-7mol/L , VitC50mg/L ） and the chondrogenic induced culture solution which included the serum of the G ranula. All groups were cultivated in 50ml cell culture bottles. The effects of the G ranula of P enetrating Bone and Removing Pain on chondrogenic phenotype differentiation of BMSCs were investigated after being cultivated for 1, 2, 3 weeks, then cells observed by inverted phase contrast microscope and immunocytochemical stain.

3将第2代SD大鼠BMSCs分为空白对照组、单纯软骨诱导剂组及透骨消痛颗粒含药血清加软骨诱导剂组，采用原培养液、软骨诱导培养液（TGF-β310ug/L，Dex10-7mol/L，VitC50mg/L）及透骨消痛颗粒含药血清的软骨诱导培养液，50ml细胞培养瓶内进行培养，1、2、3周后通过倒置相差显微镜及免疫细胞化学染色等实验技术，研究透骨消痛颗粒对BMSCs诱导成软骨细胞的影响。

In this paper, the author sets up the dynamic model of the vibratory extracting-driving piling machine, and carries out the dynamic analysis, parameter calculation and Matlab's simulation for this model. Using the equation reciprocity law to carry out the calculation of soil parameter and simulation, and using the genetic algorithm to make optimization of the machine on the basis of the simulation, the author gets the optimum self-induced vibration frequencies of the machine changing with the depth of the pile and the optimization result of other parameters, thus reaching the conclusion that along with the increase of pile sinking depth, the optimum self-induced vibration frequency becomes bigger, and the vibration mass becomes smaller, hence deepening the knowledge of the dynamic performance of this system.

本文建立了振动沉拔桩机的动力学模型并对这一模型进行了动力学分析、参数计算和Matlab仿真，并利用方程对等法进行土壤参数的计算和仿真，又在仿真的基础上使用遗传算法对桩机进行了优化，从而得出了路基加固振动沉拔桩机随沉桩深度变化而改变的最佳激振频率以及其它参数的优化结果，并得出了随沉桩深度的增加，桩机的最佳激振频率由小变大，最佳参振质量由大变小的结论，深化了对该系统动力学性能规律的认识。

In this paper, the author sets up the dynamic model of the vibratory extracting-driving piling machine, and carries out the dynamic analysis, parameter calculation and Matlabs simulation for this model. Using the equation reciprocity law to carry out the calculation of soil parameter and simulation, and using the genetic algorithm to make optimization of the machine on the basis of the simulation, the author gets the optimum self-induced vibration frequencies of the machine changing with the depth of the pile and the optimization result of other parameters, thus reaching the conclusion that along with the increase of pile sinking depth, the optimum self-induced vibration frequency becomes bigger, and the vibration mass becomes smaller, hence deepening the knowledge of the dynamic performance of this system.

本文建立了振动沉拔桩机的动力学模型并对这一模型进行了动力学分析、参数计算和Matlab仿真，并利用方程对等法进行土壤参数的计算和仿真，又在仿真的基础上使用遗传算法对桩机进行了优化，从而得出了路基加固振动沉拔桩机随沉桩深度变化而改变的最佳激振频率以及其它参数的优化结果，并得出了随沉桩深度的增加，桩机的最佳激振频率由小变大，最佳参振质量由大变小的结论，深化了对该系统动力学性能规律的认识。

In another experiment, the addition of AGE to a rat erythrocyte suspension reduced the rate of peroxynitrite-induced hemolysis in a concentration-dependent manner, suggesting that AGE protects erythrocytes from membrane damage induced by peroxinitrite.

在另一个试验中，AGE加入大鼠红细胞悬液中可以以浓度依赖的方式减少过亚硝酸盐诱导的溶血速率，提示AGE可以保护红细胞膜免受过亚硝酸盐损坏。

I will endeavour to find you some assistance.

我尽力帮你找人帮忙。

At first I only know bruck is the idol of American younglings, afterwards I returned back to Taiwan ,even in Beijing last year ,I saw her poster everywhere, I was so surprised at her charm.

起初我只晓得布鲁克雷德丝是美国少男少女崇拜的偶像，后来回台湾，甚至去年在北京，居然也四处看见她的海报，才惊讶她的魅力之大。