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In the device, a four-post frame was adopted, two synchronously rotating grip holders were used to realize rigidity rotation of the specimen, and the axial movement of the upper grip holder implemented the tension/compression loading. Positioning accuracy in the axis, radial and circumferential directions were achieved by adopting many advanced and delicate technologies. Measurement on the device indicated that the actual positioning accuracy of the device was better than the kinematic positioning accuracy. Evaluation test on the system indicated that the spatial resolution is better than the pixel size without considering the noise.

该装置采用了四柱式框架结构,采用上下夹头同步转动实现试件相对于框架的刚性转动;上夹头作相对于框架的轴向运动实现对试件的加载;在加载和转动装置中采用了诸多高精技术,实现了试件高精度的轴向定位、径向定位和刚性角度定位;对装置的精度检测表明其实际的定位精度优于运动学定位精度要求;对系统的计量考评初步表明,系统不计噪声的空间分辨率优于X射线CCD的像素10.9μm。

In use, a coil 92 is connected to the output terminal 16, and driven in an energise mode in which current in the coil 92 is built up as indicated by arrow 100, a freewheel mode in which current circulates freely as indicated by arrow 102, and then may be switched off.

使用中,线圈92与输出端16相连,并且在激励模式和空转模式中被驱动,其中在所述激励模式中,线圈92中电流沿箭头100的指示建立;在所述空转模式,电流沿箭头102指示的方向自由循环,然后切换到关断。

The result indicated that (1) During 10 years, the number of unused land and grassplot was decreased, the other types of use land were increased;(2) The absolute index of land structure variation in Aksu area during 10 years was 8, among which the annual change of garden land and traffic land was great, the annual change of grassplot and unused land was small;(3) The relative change of introduced single type of land use indicated that there was significant regional difference in the change of land use in Aksu area.

结果表明:(1)在10年尺度上土地利用总体动态特征是未利用地和牧草地减少,其它土地利用类型增加;(2)10年间阿克苏地区土地结构变化绝对指数为8,其中:园地,交通用地的年变化率较大,牧草地、未利用地的年变化率较小;(3)引入的单一土地利用类型相对变化率,反映出阿克苏地区土地利用变化存在明显区域差异。

In the event of transferring the laborer during the labor-contract term to the occupational-disease-inductive post that is not indicated in the labor contract due to the change of post or duty, the Employer should faithfully reveal to the laborer the information indicated hereinabove and consult with the laborer for modifying the related provisions of the existing labor contract.

劳动者在已订立劳动合同期间因工作岗位或者工作内容变更,从事与所订立劳动合同中未告知的存在职业病危害的作业时,用人单位应当依照前款规定,向劳动者履行如实告知的义务,并协商变更原劳动合同相关条款。

Cyclic voltammetry indicated that there were Cr→Cr, Cr →Cr oxidation reaction on anode, while there were Cr→Cr, Cr→Cr, Cr →Cr deoxidization reaction on cathode. The graphite electrodes treated by acid and heating could enhance the reversibility and kinetic of the electrode reaction. The nature graphite electrodes had been eroded during charge/discharge process, while the graphite electrodes by activation could avoide corroding by hexad chromium ion. The reversibility and kinetic of the electrode reaction of chromium ion on activation electrode had been increased, so which indicated that graphite surface augment oxygenous function corps which could increase electrode hydrophile capability.

循环伏安实验表明:铬液流电池的电极反应非常复杂,在阳极存在Cr→Cr,Cr→Cr的氧化反应,在阴极存在Cr→Cr,Cr→Cr,Cr→Cr的还原反应;热处理的石墨电极和重铬酸钾处理的石墨电极能提高电极反应的活性和可逆性;在充放电实验中未处理的石墨电极发生严重腐蚀;分别用这两种方法处理过的石墨电极具有防止六价铬侵蚀的功能;铬离子在活化处理过的石墨电极上的反应活性和可逆性增加,可以认为:重铬酸钾溶液活化处理和加热处理后的石墨表面的含氧官能团增加,电极亲水性增强。

Results Preoperative MRI classification indicated: intact annuals in 82 levels, annuals tear with intact PLL in 123 levels, PLL tear in 70 level, free protruded mass under PLL in 48 levels; on the other hands, the amount of annuals tear and free protruded mass under PLL introoperative observation were more than that preoperative MRI indicated: intact annuals in 38 levels, annuals tear with intact PLL in 165 levels, PLL tear in 62 level, free protruded mass under PLL in 63 levels; dural sac penetration in 2 levels.

结果: MRI术前分型:纤维环完整者81个椎间隙、纤维环破裂后纵韧带完整者123个椎间隙、后纵韧带破裂者70个椎间隙、髓核后纵韧带后方游离者48个椎间隙。手术发现纤维环破裂数量以及后纵韧带后方游离髓核片数量明显高于MRI的提示:纤维环完整型38个椎间隙、纤维环破裂后纵韧带完整165个椎间隙、后纵韧带破裂髓核疝62个椎间隙、后纵韧带后方游离髓核63个椎间隙、硬膜囊下疝2个椎间隙。

The resul ts indicated:(1) When micrococcus existed,carbon steel corrosion was accelerated by a loose and heterogeneous biofilm formed on its surfaces;(2)Rpo an d Cc values were close after two days and four days immersion respectively in solutions with micrococcus,which indicated that the metabolization of bacteria became sta ble,and the biofilms under two conditions were tend to be ripe and accordant;(3) The equivalent circuits for situations without or with bacteria were proposed respectively,and the parameters in circuits were also calculated;and (4) Corrosi on reaction impedance with micrococcus showed dispersion effect.

结果表明:(1)小球菌存在时试样表面上所形成的疏松、不均匀的生物膜加速了碳钢的腐蚀过程;(2)有菌存在时暴露2 d、4 d的Rpo值和C c值较为接近,表明细菌的生长代谢过程渐趋于稳定,两种暴露条件下所形成的生物膜趋向于成熟一致;(3)分别提出了有菌和无菌条件下EIS的等效电路,并计算了相应的等效元件参数;(4)小球菌影响下腐蚀反应的阻抗行为存在弥散效应。

The results revealed that nine of the fourteen Y-specific markers cannot be used for investigating the swamp buffalo Y chromosome genetic diversity because of the reasons specified below: three microsatellites markers (INRA008, UMN0103 and UMN0504) were single allele and monomorphism, three (UMN1113, UMN0304 and BC1.2) indicated three alleles respectively but mornomorphism, and the other three (UMN0920, UMN0307 and UMN3008) showed irregular ladder-like bands. Only the remaining five microsatellites (INRA124, INRA189, BM861, PBR1F1 and UMN2001) indicated polymorphisms, and thus can be applied to study the swamp buffalo Y chromosome genetic diversity.

结果表明,3个标记(INRA008,UMN0103和UMN0504)只有1个等位基因,表现为单态;3个标记(UMN1113,UMN0304和BC1.2)均为3个等位基因,但呈单态;3个标记(UMN0920,UMN0307和UMN3008)呈现无规律的梯状条带,所以这9个标记都不适用于水牛的Y染色体遗传多样性研究;只有5个标记(INRA124,INRA189,BM861,PBR1F1和UMN2001)具有多态性,表明适用于水牛的Y染色体遗传多样性研究。

We found that during the growth process the double steps first grow separately and the connected by short bridge before tangential growth. After eighth or ninth layer the spiral steps would merge into intact elliptic layers. At the merging point right-handed spiral sources might appear but the left ones always overgrow it which made the whole growth topography looked like left-handed apparently. It indicated that the EAFP molecules has strong ability to adjust itself to adapt the environment. 5. Through various analysis of interactions in the EAFP crystals it indicated that there were 218 residue interactions between 12 symmetry-related EAFP molecules around one EAFP molecule, 18 strong ones and 72 weak ones .

EAFP在低过饱和度下的螺旋生长过程中会出现台阶的合并,从而表现出单-双的螺旋台阶,在小范围扫描中捕获到了这种现象,使得对螺旋生长台阶的合并过程有了更加深入的认识;并且台阶的生长受到沿晶体学b轴方向上的21螺旋对称的控制;当台阶生长至第八到第九层时,螺旋台阶会自动地形成完整的生长层,这充分说明了EAFP分子或分子簇具有良好的自身调节能力,这样在晶体生长过程中,晶体的缺陷减少,晶体质量提高,这也可说明EAFP晶体的x射线衍射能力很强的原因。

It also can be used as alternative antigen of newgeneration vaccine.In this experiment,we screen the major protective antigen hsp65 gene of MAP in order to developnew vaccine especially the DNA vaccine for the prevention of paratuberculosis disease.The hsp65 genewas amplified from MAP C-2 chromosomal DNA by using the PCR technique.We gained a hsp65 gene of 1 626bp.Then PCR product was cloned into pGEM-T vector by T-A clone technique and therecombinant clone was identified by plasmid size,enzyme digestion and PCR identification.The cloneplasmid of pGEM-T- hsp65 was successfully constructed.The nucleotide sequence and deduced aminoacid sequence ofclone gene was analyzed by DNASTAR software.The result indicated that the size ofhsp65 gene consist with M.paratuberculosis K-10 strain in GenBank and the sequential homogeneityreached 99.1%,the amino acid homogeneity reached 99.3%.The preceding analysis indicated that thehsp65 gene was very conservative in M.paratuberculosis.

为了研发预防副结核病的新型疫苗尤其是DNA疫苗及相关蛋白功能,本研究选择了MAP的主要保护性抗原Hsp65蛋白,以副结核分枝杆菌C-2株染色体DNA为模板,以hsp65基因的特异性引物进行PCR扩增,获得了1 626bp的hsp65基因,通过T-A克隆技术,将PCR产物克隆至pGEM-T Vector中,以质粒大小、酶切分析、PCR扩增及序列分析鉴定重组克隆,成功地构建出克隆质粒pGEM-T-hsp65,以DNASTAR软件分析了所克隆基因的核苷酸序列和推导的氨基酸序列,结果表明,所获得的hsp65基因与GenBank中MAPK-10株该基因核苷酸大小完全一致,两者核苷酸序列的同源性为99.1%,氨基酸序列的同源性为99.3%,表明该基因在副结核分枝杆菌中高度保守。

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