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METHODS: According to adherent + Thy1.1 antibody and complement-purification method, cranium was opened to expose olfactory bulb. Thereafter, two olfactory bulbs were obtained to remove cerebral pia mater, blood capillary, and peripheral tissues; additionally, olfactory nerve layer and olfactory bulb granular layer were sheared into 1-mm3 pieces for extract single-cell suspension. The cells were adjusted at the density of 1×107 /L and incubated with poly-l-lysine-coated culture bottle or culture plate in 5% CO2 incubator at 37 ℃. On the third day, cells were cultured with serum-free DMEM/F12 culture media.

在差速贴壁+Thy1.1抗体及补体纯化法的基础上,剪开大鼠颅骨,显露位于颅腔前方的嗅球,取出2只嗅球,在显微镜下去除嗅球表面的软脑膜和毛细血管及外周组织,保留富含嗅鞘细胞的嗅神经层和嗅球颗粒层,剪成1 mm3小块分离获取单细胞悬浮液,调整细胞密度至1×107 L-1,接种在用poly-l-lysine包被的培养瓶或培养板中,于37 ℃、体积分数为5%的CO2培养箱中培养,第3天用无血清DMEM/F12培养基换液培养。

This paper brings forward some new viewpoint and series of advices to the development of technique enterprise incubator and hope it can foster its development and combines technique and economy together and facilitate the transformation of technical product and its industrialization.

本文提出了一些新的观点,针对科技企业孵化器的发展也提出了一系列的建议和意见,希望对加快我国的科技企业孵化器的发展,加强科技与经济的结合,促进科技成果的转化和产业化,加快高新技术产业的发展,能起到积极的作用。

Huating Sunshine Oasis"is the representer of high-quality estate of the best lot in downtown of Wuhu;the international franchise "Darunfa Market"is the real estate project that is specially developed by Huayi domain. Huayi International New City Item is a large real estate project of 600 acres which gathers residential,luxious hotel,luxious office,shopping centre and supermarket together."Huayi Scientic Gardonis the typical of real estate industry and one of the best item of incubator equipment in East China.

"华亭阳光绿洲"是芜湖市中心代表黄金地段高品质楼盘的代表;国际连锁"大润发超市"是华亿置业精心打造的商业地产项目;"华亿国际新城"项目是集住宅、高档酒店、高档写字楼、购物中心、大型超市于一体的600亩大型地产项目;合肥"华亿科学园"是产业地产的经典和华东地区最佳的孵化器项目之一。

Some import incubator circuits are single route check ,2 step control Temperature sensor is unusually adopted the voltage concentration circuit .

而有些进口是单路检测、二级控制电路)温度传感器是先进的电压型集成电路温度传感器,而不是常用的热敏电阴、半导体、热电偶等传感器。

Space-saving low temperature incubator is ideal for small volume workloads.

节省空间的低温培养箱主要用于小工作量的实验。

A basic knowledge of microbiology, will be detected with a microscope observation of micro-organisms, medium Preparation, disinfection and sterilization, separation and purification of micro-organisms, bacteria counting method A chemical analysis of the theoretical knowledge and can use chemical analysis methods and equipment of the components of material analysis Master fermentation process principles, understanding of beer, liquor, fermentation production technology Master protein related knowledge, PAGE gel electrophoresis technology, high-speed centrifugal Genetic engineering master the basic theory of knowledge, the extraction and purification of genes, PCR principle, gene recombination technology Master of the basic theoretical knowledge, the immobilized enzyme, the enzyme preparation Application Master of animal and plant tissue culture techniques of experimental steps studied experimental apparatus: Balance (scales, electronic scales, Analytical Balance), or acidity, spectrophotometer, liquid gas chromatography, acid-base titration apparatus, microscopes, centrifuges, ultrasonic cleaning, constant temperature oscillation incubator, super-clean table, steam sterilization pot, the pot constant temperature water bath, PAGE device apparatus, oven, fermenter, such as PCR instrument.

具备微生物理论基础知识,会用显微镜检测观察微生物,培养基的制备、消毒与灭菌,分离与纯化微生物,细菌的计数法具备分析化学的理论知识,能运用化学分析和仪器分析方法对物质的组分进行分析掌握发酵工艺原理,了解啤酒、白酒、发酵生产工艺掌握蛋白质相关知识,PAGE凝胶电泳技术,高速离心掌握基因工程的基本理论知识,基因的提取和纯化,PCR技术原理,基因重组技术掌握酶的基本理论知识,酶的固定化方法,酶制剂的应用掌握动植物组织培养技术的实验步骤学过的实验仪器:天平(台天平,电子天平,分析天平)、酸度计、分光光度计、液气相色谱仪,酸碱滴定仪器,显微镜,离心机,超声波清洗器、恒温振荡培养箱、超净工作台,蒸气灭菌锅、恒温水浴锅,PAGE电泳装置仪器、干燥箱、发酵罐、PCR扩增仪等。

As for subsidiary facilities and services, there is a public technical service center, a customized property maintenance department, an intellectual property rights exchange market, a government industry association, a 3000㎡-creative business assembly hall, a serviced business apartment, a creative idea incubator, a display and product releasing center, a meeting center, an industrial and commercial agency, public accounting firms, a print and copy center, personnel service, industry information service, network and communication system installation and maintenance service, which as a whole provide all-round services.

在产业设施方面,设立公共技术服务中心、管家式物业服务部门、知识产权交流场所、政府行业组织、3000平方米的创意商务会馆、酒店式商务公寓、创意孵化中心、展览发布中心、会议中心、人事服务、产业信息服务、工商代理机构、会计事务所、印务中心、园区网络通讯系统安装维修服务等等,服务配套齐全。

This paper firstly introduces the deep excavation's engineering situation,geological and hydro-geological conditions of Wuhan University's Science and Technology Incubator Building,and mainly introduces design train of thoughts of choosing different timbering precepts according to field's circumjacent condition.

首先介绍了武汉大学科技孵化器大楼深基坑工程的工程概况及工程地质水文地质条件,重点介绍了根据场地周边条件的不同选择不同支护方案的设计思路。

You'll see how your incubator can tap into this market and create opportunities for underserved entrepreneurs in your community.

你将知道你的孵化器将怎样帮助他们创建机会进入市场。

Ningbo Golden Georg specialize in the production of household electrical appliances are electrical products, such as: an incubator, mini refrigerators, wine cooler, etc. In the past few years we have been committed to the development of new products in order to meet customers various demands.

宁波金格奥电器是专门生产家用的电器产品如:保温箱、迷你冰箱、酒柜等等,在过去的几年里我们一直致力于开发新的产品以此来满足客户各方面的要求。

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Moth In The Incubator
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In the United States, chronic alcoholism and hepatitis C are the most common ones.

在美国,慢性酒精中毒,肝炎是最常见的。

If you have any questions, you can contact me anytime.

如果有任何问题,你可以随时联系我。

Very pretty, but the airport looks more fascinating The other party wisecracked.

很漂亮,不过停机坪更迷人。那人俏皮地答道。