查询词典 incubation

The cells were incubated with Alexa 488 conjugated Staphylococcus aureus bioparticles opsonized with rabbit IgG for 1h, followed by fixation and incubation with Alexa 633 conjugated goat anti-rabbit IgG to distinguish ingested from extracellular bioparticles.

将兔抗IgG 作用于Alexa 488金黄色葡萄球菌的活性颗粒，后与实验细胞(上述4种)孵育1小时，再用Alexa 633羊抗兔IgG定位，以区别活性颗粒是被吞噬的还是位于细胞外的。

The cells were incubated with Alexa 488 conjugated Staphylococcus aureus bioparticles opsonized with rabbit IgG for 1h, followed by fixation and incubation with Alexa 633 conjugated goat anti-rabbit IgG to distinguish ingested from extracellular bioparticles.

将兔抗IgG 作用于Alexa 488金黄色葡萄球菌的活性颗粒，后与NTM细胞孵育1小时，再用Alexa 633羊抗兔IgG定位，以区别所吞噬的位于细胞外的活性颗粒。

Fibroblasts were passaged with 0.25% trypsin.After 24 and 48 hours incubation,MTT was used to measure the cell proliferation.

取第3代MCL细胞，培养24、48 h后，采用二甲基噻唑二苯基四唑溴盐法测定细胞增殖。

METHODS: BMSCs were obtained from Japanese big-eared rabbits, and in vitro cultured. Then the subcultured BMSCs were transfected by pCDNA3.1 plasmid, followed by incubation on swine SIS to construct the tissue-engineered skin. The growth of cells and phenotype of BMSCs were detected by flow cytometry.

日本大耳兔骨髓间充质干细胞进行体外培养扩增后，通过pCDNA3.1质粒将碱性成纤维细胞生长因子基因转染至生长状态良好的骨髓间充质干细胞，并将转染后的细胞接种于制备好的猪小肠黏膜下层上，进行体外联合培养，构建组织工程皮肤。

Objective To know if MCF-7 breast cancer cell line has breast cancer stem cell,and change of stem cell pool in diverse incubation circumstance.

以及在不同的培养条件下，干细胞池的变化情况。

Methods The chemical hypoxia was induced by incubation of U251 cells with cobalt chloride.

以氯化钴模拟缺氧，采用RT-PCR、免疫细胞化学方法分别检测缺氧诱导因子-1α和血管内皮生长因子mRNA和蛋白的表达，鸡胚绒毛尿囊膜模型检测缺氧时胶质瘤细胞血管生成情况。

The results showed that the amount of nitrogen mineralized from the green manure added to soil was corelated with the effective cumulative temperature i.e.y=k[(T—15)D]~n in which y is the amount of nitrogen mineralized, D the number of days, T the actual temperature during incubation and k and n constants.

结果表明，在20—30℃淹水培养条件下，加入土壤中的绿肥氮矿化率与有效积温(T—15)D的关系，符合y=K[(T一15)D]~n经验公式的规律。

Under the optimum concentration of 1.5% glycerol in fermentation medium and feeding of 1% glycerol as precursor after 60 h of incubation, the titer of 2,668 μg/ml was obtained in shake flask culture, an increase of 7.4 times compared with the original strain.

以XC-5-48菌株为试验菌株，当培养基中甘油初始浓度为1.5%，并且在发酵60h时补加1.0%的甘油，摇瓶发酵的效价可达2668μg/ml，较出发菌株(318μg/ml)提高了7.4倍。

After 72 hours incubation, the chondrocytes were digested into single cell suspension. Then they were incubated in the 96-well culture plate with the density of 1×10^8 L^(-1), 100 μL per well and six wells for per group.

白细胞介素1β加入量为5μg/L，培养72h将实验用细胞消化传代后制成单细胞悬液，以1×10^8L^的密度接种于96孔培养板，100μL/孔，每组6孔。

Biodegradation and degradation kinetics of anion-surfactant in the anaerobic water of a representative inlet of Lake Dianchi under different incubation conditions were studied by the'river die-away'test method.

LAS 的结构与生物降解性的关系[10 ，11 ] ；②利用驯化培养的高效菌株来降解废水中高浓度的LAS[12～14 ] ；③ LAS 在水处理系统中的生物降解[15～17 ] 。

They weren't aggressive, but I yelled and threw a rock in their direction to get them off the trail and away from me, just in case.

他们没有侵略性，但我大喊，并在他们的方向扔石头让他们过的线索，远离我，以防万一。

In slot 2 in your bag put wrapping paper, quantity does not matter in this case.

在你的书包里槽2把包装纸、数量无关紧要。