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The supernatant was removed and 100 μL dimethyl sulphoxide was added to each well for 4 additional hours of incubation. In addition, some cells were exposed to bFGF and EGF.

另外，一部分细胞用碱性成纤维细胞生长因子和表皮生长因子单独或联合对其进行诱导分化。

Recovery studies on the TEER showed that the effect of the LV and LTV vaccines on Caco-2 cell monolayer is reversible and proves the viability of cells after incubation with all vaccines.

而在TEER的恢复实验中，可以观察到LV与LTV组给予Caco-2之后，再给予细胞培养后细胞间紧密联合可以恢复，细胞也能存活。

Under the support of the National, Guangdong and Guangzhou industrial incubation project, we have set up the biggest manufacture system and technology in China, which are even seldom seen in the world. We own: 2000L liquid fermentation industry system; 3 national invention patents including its manucfature and transport tenology Two types of entomathogenic nematode have got the import permission from the Agriculture Department of United States. Advanced methods of culture media and liquid cultivation conduce to the three times of production compared with other nematode commercial companies in the oversea, as well as the high quality control and transport technology are contributed to the firm fundamention of commercialization.

在国家，广东省与广州市等一系列产业化孵化项目的资助下，我们现已建立了年产50万亿线虫（可用于100万亩苹果小食心虫的防治），目前国内规模最大，国际上为数不多的生产系统和生产技术；拥有： 2000L 液体发酵生产系统；昆虫病原线虫产业化生产和储运技术等自主知识产权（3项国家发明专利）；两个线虫产品已获美国农业部核准的&准入&许可优化的培养基配方和液体培养方案，可获得比国外线虫商业公司专利培养基高出三倍的线虫产量，以及完善的产业化质量控制技术和贮运技术，这些均为这类生物杀虫剂的产业化奠定了坚实基础。

After incubation,it was found that (1)the iodine uptake by the thyroid,(2)the conversion of inorganiointo organic iodine,(3)the thyronine synthesized and(4)the thyroid peroxidase activityin the LATS-IgG...

如果先用~(125)I体内标记2小时，再将甲状腺摘出体外培养，则LATS组不仅总的释放率增加，而且释放物的组成成分，也有所不同，激素碘占16.6%，无机碘占83.4%；而对照组分别为2.1%和97.9%。

According to the above results, feather, tryptone, yeast extract as well as incubation time and temperature were selected for further studies by RSM. Data obtained showed the maximal biomass production and keratinase activity occurred at above 96 h.

根据上述结果选择feather、tryptone、yeast extract外加两个物理因子进行第一次的RSM分析，结果发现欲得到较高的角蛋白酶活性与菌体产量，其培养时间需超过四天以上。

The characterization of photobiological hydrogen production for four marine green algae by sulfur-deprived and treated with uncoupler after dark anaerobic incubation was investigated.

分别采用培养基中无硫连续光照和厌氧暗诱导后添加解偶联剂光照的方法，研究几株海水绿藻的光照产氢特征。

Two techniques were compared that uses the UV absorbance at 280nm of 0.5mol/L K_2SO_4 extracts of fumigated and unfumigated soils and fumigationwaterlogged incubation to estimate the concentrations of nitrogen in the SMB.

用氯仿熏蒸-0.5mol/L的K2SO4直接浸提，280nm紫外比色法和熏蒸-淹水培养法测定了20种有机质、全氮和速效氮差异较大的土样的土壤微生物量N。

Results Incubation of TEC with MAC increased the expression of collagen type Ⅳ(P.05) in comparison with unstimulated cell.Conclusion MAC can i...

结论亚溶破剂量的MAC能使HKC的Ⅳ型胶原表达上调，可能是引起肾小管间质纤维化细胞外基质增多的原因之一。

ResultsTMZX granules obviously increased the urine volume of mice and rats, prolonged the incubation period of pigeon, and decreased the vomitive times of pigeon.

结果天麻止眩颗粒有明显的利尿作用；能延长硫酸铜和顺铂致家鸽呕吐的潜伏期，并能减少硫酸铜和顺铂所致家鸽的呕吐次数。

Methodsthe diuretic effect was studied by weighing method and metabolic cage to observe the urine volume of mice and rat;the emesis induced by both bluestone and cisplatin were studied by observing the incubation period of emesis of pigeon and the vomitive times of pigeon.

方法采用称重法和代谢笼法研究受试药对小鼠及大鼠的利尿作用；采用硫酸铜和顺铂致家鸽呕吐的模型，观察受试药的镇吐作用。

They weren't aggressive, but I yelled and threw a rock in their direction to get them off the trail and away from me, just in case.

他们没有侵略性，但我大喊，并在他们的方向扔石头让他们过的线索，远离我，以防万一。

In slot 2 in your bag put wrapping paper, quantity does not matter in this case.

在你的书包里槽2把包装纸、数量无关紧要。