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During the ESR study of the effects of oxLDL on the ESR signal intensity of hydroxyl radicals in collagen (2 μg/ml)-activated platelets, we found that incubation of human platelets with collagen produced a typical four-line hydroxyl radical signal and a long-lived g = 2.005 radical detectable by spin trapper 5,5-dimethyl-1 pyrroline N-oxide.

在研究oxLDL降低由collagen 〈2 μg/ml〉刺激血小板产生的氢氧自由基〈OH●〉的ESR讯号强度的同时，我们发现以DMPO当自由基捕捉剂，collagen除了刺激血小板产生一个四线的典型氢氧自由基之外，还产生一个长半衰期 g = 2.005的自由基。

The characteristics of EIS of rebar in periods of stable passivation, pit incubation and pit propagation were studied.

研究了钢筋在不同pH和Cl~-浓度的混凝土模拟液中的电化学阻抗谱。

In an informationized academic environment,through a shift from mere course teaching to innovative teaching integrating information technology and academic learning to put the incubation of information accomplishment dominantly or recessively through the course teaching will be a major approach to cultivate students′ information accomplishment.

在学校教育中，重视和加强信息素养教育，培养学生在多媒体和网络环境中高效学习的方法与能力，既是时代的要求，也是信息化社会教育改革的基本方向之一。1 信息素养的内涵和特征信息素养的概念是从图书检索技能演变发展而来的[1]，1989年美国图书馆协会ALA

Add the resazurin sodium solution, mix, and place the medium in suitable vessels that provide a ratio of surface to depth of medium such that not more than the upper half of the medium has undergone a color change indicative of oxygen uptake at the end of the incubation period.

加入刃天青钠溶液，混匀，并将该培养基置于适当容器中，该容器应为培养基提供特定的面积－深度比，以使在培养期末表明氧气摄入的变色部分不超过培养基的上半部分。

Our data are showed that apoptosis parameters including DNA fragmentation, nuclear morphological change and sub-G1 fraction, appeared after 24 h by 5 μM rottlerin treatment. Furthermore, rottlerin caused a decrease in 72% of ODC protein expression and an increase in threonine, rather than serine and tyrosine dephosphorylation on ODC enzyme compared with the control after incubation for 10 h. To determine whether ODC could down-regulate rottlerin-induced apoptosis, HL60 cells were stably transfected with ODC cDNA. HL60-ODC cells exhibited a 2.5-fold increase in ODC protein compared with vector only (HL60-pcDNA3) or HL60 cells.

在我们筛选天然化合物实验中，发现rottlerin抑制鸟胺酸去羧化活性最具成效，之前的研究人员已知蛋白激Cd调节鸟胺酸去羧化的活性，因此在人类白血病HL60细胞处理rottlerin的实验中发现加入rottlerin二十四小时后，细胞进行计划性死亡，并可侦测到DNA片段、细胞核浓缩及sub-G1提增；而细胞处理药物十小时后与控制组细胞比较，观察到鸟胺酸去羧化的蛋白表现与活性下降50﹪以上；除此以外，测定鸟胺酸去羧化去磷酸化的情况，发现於酥胺酸位置有显著差异，而丝胺酸和酪胺酸位置并无差异。

Second, residues in the reactive site of the inhibitor were replaced by the consensus substrate recognition sequence of furin, namely, Arg at P_1, Lys at P_2, Arg at P_4 and Arg at P_6. In addition, the P_7 residue Asp was substituted with Ala to avoid possible electrostatic interference with furin inhibition. Finally, the extra N and C terminal residues beyond the doubly conjugated disulfide loops were further truncated. However, all resultant synthetic peptides were found to be temporary inhibitors of furin and kexin during a prolonged incubation, with the scissile peptide bond between P1 and P'1 cleaved to different extents by the enzymes.

高活性、低分子量的furin酶抑制剂有可能发展成为新型的抗细菌内毒素和抗病毒感染的药物，我们以绿豆胰蛋白酶抑制剂Lys片段长链22肽为模板，设计了一系列能抑制furin和kexin活性的突变体，采用如下三种方法逐步优化：一、去除Lys片段长链三对二硫键中的一对，用Ser替换Cys，减少可能存在的二硫键错误配对；二、抑制剂的活性中心根据furin底物的专一性逐步改变，即P1、P2、P4和P6位分别变为Arg、Lys、Arg和Arg，同时为了避免P7 Asp和P6 Arg之间可能存在的相互作用，将P7位Asp替换为Ala；三、将两对二硫键形成的双环的外面5个氨基酸残基去除。

In a two-step incubation experiment, high and low temperatures were combined with RH 70%, RH 80% and RH 90% to treat two silkworm races, Bibo x Dongtin and Chunlei X Zhenzhu, to investigate the causes for uhe occurrence of reversed silkworm in sericultural production and its hatching pattern.

针对生产上出现&逆出蚕&这一情况，通过催青实验调查发生逆出蚕的原因及其孵化方式。实验采用碧波×洞庭、春蕾×镇珠 2个蚕品种，分别在常温、高温与RH 70 %、RH 80 %、RH90 %组合的 6个条件下，进行两段式催青至孵化。

During the incubation phase, lasting up to 72 hours, the vampirism disease exhibits no symptoms, and may be cured by general spellcraft or cult blessings.

72小时的潜伏期间内，vampirism病不会显示任何征兆，使用治疗魔法或接受教团的祝福可以治疗此病。

Under the condition of laboratory incubation,the C loss of organicmaterials during their mineralization was greater than their N loss,inducing ahigher residual amount of organic organic N.During the decay of maize andrice staw with a wide ratio of C/N.soil microbes could fix N,but the fixationrate was quite low,only 0.2-0.9g N/assimulated 100g organic C.

在实验室培养条件下有机物料在矿化分解过程中C的损失总是大于N的损失，因此有机N的残留率明显高于有机C的残留；且由于灰分总量不变，有机物料的残留率也略高于有机C的残留率；〓比较宽的玉米秸和稻秸在腐解过程中发生了自生固N作用，但其固N效率仅为C：N=100：0.2-0.9，即每同化100g有机C，只能固定

Conversely the seeds had the longest mean length of incubation time and germination peak value when pollinated every day.4 The planting date had effects on yield and quality of carrot seeds from different seed umbels and poisons of seed disc, also had effects on the growth and development of carrot steckling.

不同授粉时期对种子产量、千粒重、发芽率、发芽势的影响不显著，仅对种子的平均发芽天数、发芽高峰日分别存在显著、极显著影响，且&每日授粉&的种子平均发芽天数及发芽高峰日最长，&隔1日授粉&的种子平均发芽天数及发芽高峰日最短。

Plunder melds and run with this jewel!

掠夺melds和运行与此宝石！

My dream is to be a crazy growing tree and extend at the edge between the city and the forest.

此刻，也许正是在通往天国的路上，我体验着这白色的晕旋。