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METHODS:Calcification of cultured rat VSMCs was produced by incubation with β-glycerophosphate. Calcium content, Ca2+ deposition and alkaline phosphatase activity were analyzed to estimate the extent of calcification.

目的：在离体钙化的血管平滑肌细胞上，观察内皮素受体阻断剂对血管平滑肌细胞钙化的影响，探讨内皮素促进细胞钙化的信号转导和分子机制。

The soil pH was found to be the main factor affecting BR formation of 14 C\|metsulfuron methyl among the basic properties of soil.(2)During the whole periods of the incubation,the 14 C\|BR of 14 C\|metsulfuron methyl in soils was mainly distributed in fulvic acid and humin.The relative percentage of 14 C\|BR in fulvic acid was higher than in humin,while the content of the 14 C\|BR distributd in humic acid only account for less than 1% of applied amount.

土壤pH是14 C 甲磺隆在土壤中形成BR的主要影响因子。14 C -甲磺隆在各类土壤中的14 C BR的最大值分别占引入量的 48 5%、46 5%、52 6%、1 9 3 %、49 7%、42 0 %和 46 5%；( 2 )在整个培养试验过程中，14 C 甲磺隆在 7种不同类型土壤中的14 C BR ，主要分布在富啡酸和胡敏素中，前者中的相对百分比大于后者，而在胡敏酸中的相对百分比较小。

It was found in this investigation that boron exerted considerable influenceon the nucleation and growth of pearlite. In a hypoeutectoid steel, boron delayedthe starting time of pearlite formation at the maximum pearlite formation temp-erature. Boron did not effect the relation of nucleation rate with time butonly increased the incubation period for pearlite nucleation.

从实验结果证明，硼对珠光体的成核及成长是有影响的；在亚共析钢中硼促使珠光体的开始形成时间推迟，增长了珠光体成核的孕育期，但对成核率与时间的关系，在近于A_1温度(700℃)时，略为加速，而在珠光体转变速度最大的温度(650℃)时却没有影响。

The culture ingredients and conditions were optimized with the orthonormal table L_9(3~4) method. Studies showed that the culture components for optimization of β-mannanase production were yeast extract 20 g/L, Konjak powder 20 g/L,(NH_4)_2SO_4 2 g/L, KH_2PO_4 1 g/L and MgSO_4 · 7H_2O 1 g/L; and the optimal culture conditions were seed incubation time 8-12 h, inoculation ratio 6%, culture volume 25 mL, pH 7.5, fermentation temperature 40 ℃ and fermentation time 25 h.

通过正交设计方法筛选优化该菌培养基配方和产酶条件，培养基配方为：酵母抽提物20g／L、魔芋粉20 g／L、(NH_4)_2 SO_4 2 g／L、KH_2PO_4 1 g／L、MgSO_4·7H_2O 1 g／L；产酶条件为：菌种种龄8～12h、接种量6％、装液量25 ml、pH7.5、培养温度40℃、培养时间25h。

A 30 min exposure to peroxynitrite (10〓μmol/L) followed by incubation in fresh medium induced apoptosis in cultured cerebellar granule cells, which was confirmed by DNA laddering, chromatin condensation, nuclei fragmentation, and the formation of apoptotic bodies.

原代小脑颗粒细胞经10μmol/L过氧化亚硝基处理30min，并继续培养一定时间后逐渐发生细胞凋亡，主要表现为DNA链断裂、染色质凝缩、核破裂、形成凋亡小体。

The nymph incubation period, Lagerstroemia Eriococcus resistance is weak and the prevention and treatment of the critical period.

4，若虫孵化期，紫薇绒性薄弱，预防和治疗的关键时期。

Eight weeks swimming could increase glucose uptake and phosphorylation of IRS-1 in rat lipocyte after RBP4 incubation respectively, and improve RBP4-induced insulin resistance.

RBP4 孵育后，运动组大鼠脂肪细胞IRS- 1 磷酸化程度较安静对照组显著

Effect of percutaneous penetration ,including influence of different formulations on penetration,and lipolytic activity of Siloxanetriol alginate were studied using the techniques of percutaneous penetration in vutro and adipose tissue incubation in vitro.

利用体外经皮渗透和脂肪组织体外培养技术，探讨了硅烷醇C 经皮渗透和配方的促渗透，以及对脂肪组织的脂解作用。

A laboratory incubation experiment was conducted to study the effect of soil moisture on N2O flux from loessial soil in northwest China.

通过室内模拟试验，研究湿度对西北地区黄绵土N2O排放的影响。

RESULTS: hADSCs presented fibroblast-like morphological feature with a flocked array. The 3rd passage of hADSCs had unique immunophenotypes and they were positive for CD73, CD44, CD166, CD105 and CD29, but negative for CD31, CD34, CD45 and HLA-DR. Cell cycle result showed that they had the typical growth characteristics of stem cells, namely, 83.81% cells stayed at G0/G1 stage, only 16.19% cells were stayed at S+G2/M stage; The latent phase of the primary culture cells was 2 days prior to and after incubation, followed by 3-6 days of logarithmical proliferation, reached a peak at day 6, and entering the growth platform phase with lower growth speed.

结果：分离培养的人脂肪间充质干细胞为成纤维细胞样，呈漩涡状排列。P3代人脂肪间充质干细胞呈CD73，CD105，CD166，CD44及CD29阳性，而CD31，CD34，CD45及HLA-DR阴性；具有典型的干细胞增殖特点，83.81%细胞处于G0/G1期，仅16.19%细胞处于S+G2/M期；细胞在接种后前2 d处于生长潜伏期，第3~6天处于对数生长期，第6天达峰值，以后细胞生长速度减慢，进入生长平台期，平均五六天传代1次。

Plunder melds and run with this jewel!

掠夺melds和运行与此宝石！

My dream is to be a crazy growing tree and extend at the edge between the city and the forest.

此刻，也许正是在通往天国的路上，我体验着这白色的晕旋。