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Results Many endothelial cells were observed with higher vigor and adherance to the wall after incubation for 3-5h, and spreaded to form cell masses for 12-24h, and grew densely and converged into single layer which characteristically arranged to cobble-stone shape after incubation for 1-2 weeks.

结果 原代细胞培养3~5h后，大部分活力较高的内皮细胞已开始贴壁，12~24h后，内皮细胞己铺展形成由数个细胞组成的细胞群，1~2w后，细胞呈生长密集状并汇合形成单层，在倒置相差显微镜下，呈特征性"鹅卵石状"或"铺路石状"镶嵌排列生长。

The cytoplasmic trehalose concentration in red blood cells, free hemoglobin and ATP level were determined at different incubation temperatures (4, 22 and 37℃), different trehaolse concentrations (0, 200, 400, 600, 800 and 1000 mmol/L ) and different incubation times (2, 4, 6, 8 and 10 hours), the cytoplasmic trehalose, free hemoglobin, hemoglobin and mean corpuscular volume in fresh RBCs and RBCs strored for 72 hours at 4℃ were compared, when loading condition was ensured.

在不同孵育温度（4、22和37℃）、孵育时间（0、2、4、6、8、10小时）、不同负载缓冲液浓度（0、200、400、600、800、1 000 mmol/L）条件下检测新鲜红细胞对海藻糖的成功负载量及红细胞各项理化指标；在固定负载条件下，对新鲜红细胞和4℃保存72小时红细胞海藻糖负载、游离血红蛋白、血红蛋白和红细胞平均体积进行了比较。

Simplicissimum H5 was the most powerful degrader, which degraded 40.26% kraft lignin after 13 days incubation. In the 20-day incubation period, the LiP and Lac were secreted by P.

事实上在土壤、堆肥等系统中木质纤维素的转化绝不仅仅是高等担子菌作用结果，许多丝状低等真菌的作用不容忽视[1，2 ] 。

According to the experts concerned, because the place where Wang met snakes is damp, exposed to the sun, and with scattered rocks, this is a ideal base to breed snakes. And the snakes are fond of living gregariously during incubation. That time is just an ideal incubation period.

有专家说，王志洲遇见蛇群的地方潮湿、向阳、有岩石，是蛇群理想的繁育基地；而且孵化期中的蛇很喜欢群居，是一种自然现象。

This article demonstrates all kinds of techniques of fine-turning incubation temperature during incubation to keep highest hatchability

本文阐明了孵化温度的各种微调技术，以保持最高的孵化率及雏鸡质量。

At 2 900 m altitude,incubation with adding oxygen during incubation reduced embryonic mortalities during early and later phase,and increased hatchability.

但他们都是研究较高的海拔环境，实际生产中很少在这样高的海拔进行大量的孵化生产。

In Hi-Tech Incubation Park and the Western District Base, gymnasium was respectively installed to provide relaxing places for staff. In Hi-Tech Incubation Park, the Red Flag Multiple Shop was open and coffeehouse supported the service of buffet dinner in the noon, it supported the convenience for staff. Law Service Office was set up to provide effective service for enterprises.

高新孵化园、西区孵化园健身房启用，为广大企业员工提供锻炼身体休闲娱乐的场所；高新孵化园红旗连锁店开张，方便企业购物的需要；高新孵化园综合服务楼咖啡厅推出中西式自助餐服务，满足了企业不同进餐群体的需求；高新孵化园成立法律服务工作室，采用分工协作的方式组成律师工作团，为企业提供快捷、有效的服务。

In Hi-Tech Incubation Park and the Western District Base, gymnasium was respectively installed to provide relaxing places for staff. In Hi-Tech Incubation Park, the Red Flag Multiple Shop was open and coffee house supported the service of buffet dinner in the noon, it supported the convenience for staff. Law Service Office was set up to provide effective service for enterprises.

高新孵化园，西区孵化园健身房启用，为广大企业员工提供锻炼身体休闲娱乐的场所；高新孵化园红旗连锁店开张，方便企业购物的需要；高新孵化园综合服务楼咖啡厅推出中西式自助餐服务，满足了企业不同进餐群体的需求；高新孵化园成立法律服务工作室，采用分工协作的方式组成律师工作团，为企业提供快捷，有效的服务。

Results (1) Lens epithelial cell apoptosis began before lens opaqueness (lens became opaque after 6 hours of incubation, while the lens epithelial cell apoptosis was detected after 3 hours of incubation);(2) Taurine could apparently inhibit H2O2-induced bovine lens epithelial cell apoptosis.

结果 晶状体上皮细胞凋亡发生于晶状体混浊之前；牛磺酸可明显抑制H2O2所致的牛晶状体上皮细胞凋亡。

The steps are as the following:1、Preparing the cerebrospinal fluid one hour before the experiment,take some of them for cryopreservation;2、Without paralyse,cuting down the rat head quickly and dislodge the hippocamp part which is used in the experment in freezing liquid,and trim the hippocamp distrct.3、Fixing the hippocamp tissue on the vibratome,to cut down a brain slice with 300μm,use the haustorial tube to move the slice to the preincubate dish,cultivanting it for one hour.Then move it to the record incubation chamfer.4、Preparing the glass micloelectrode and fill it with NaCl,the corcentation is 3mol/L.5、adjusting the recorder,after cultivate the brain slice in the incubation chamfer for 2 hours,the expermentize can be gone on.6、In order to ensure the accurate of the experimental result,use only one medicine concentration for one brain slice in every experiment.

本实验研究方法是：1、实验前1小时配制好所需脑脊液并充以95% O2和5% CO2的混合气，取小部分冷冻保存；2、在乙醚麻醉下，快速断头并在冷冻液里取出包含海马的大脑部位，修整出所需海马区；3、将海马组织固定于振动切片机上切出300μm厚的海马脑片，用广口吸管转移至预孵育皿培养一小时，后再转移到记录用孵育槽内；4、拉制好玻璃微电极，并充以3mol/L的NaCl；5、调试好记录仪器，将脑片在孵育槽内培养2小时后进行实验；6、为了保证实验结果的准确性，每一块脑薄片只进行一个浓度的药品实验。

I am accused of being overreligious," she said in her quiet, frank manner,"but that does not prevent me thinking the children very cruel who obstinately commit such suicide.""

客人们在卡罗利娜·埃凯家里，举止就文雅一些，因为卡罗利娜的母亲治家很严厉。

Designed by French fashion house Herm è s, this elegant uniform was manufactured in our home, Hong Kong, and was the first without a hat.

由著名品牌 Herm è s 设计，这件高贵的制服是香港本土制造，是我们第一套不配帽子的制服。