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Methods:Rat hepatocytes were isolatcd by a single two step perfusion method,placed in 96 hole tissue plates and incubated for 1.5 hours,then exposed to different concentrations of HGF for 48 hours and the proliferation of rat hepatocytes was determined by MTT colorimetric assay.

利用胶原酶原位二步灌注法分离大鼠肝细胞，接种并预培养 1 。5 h后，用含不同浓度 HGF的培养基继续培养至 48h，MTT比色法检测肝细胞生长因子对原代培养大鼠肝细胞增殖的影响。

Methods The β-sitosterol and/or total protein of Pinella were incubated at different concentrations with cervical cancer cell line SiHa. The effects of β-sitosterol and/or total protein of Pinella on the viability of cells were tested by MTT assay.

在子宫颈癌细胞SiHa的培养基中加入不同浓度的β-谷甾醇和／或掌叶半夏总蛋白培养1~7d，采用MTT比色法检测细胞的活性。

Methods The mRNA and protein expression levels of NCAM were detected by reversed transcriptive polymerase chain reaction and Western blot after the rat hippocampal neurons were incubated with 20, 40 and 80μg/ml sodium fluoride for 24 hours in vitro.

原代培养海马细胞暴露于20、40、80μg/ml氟化钠24 h后，用RT-PCR和Western blot方法分别检测细胞内NCAM mRNA和NCAM各蛋白亚型的表达。

Methods After L-02 cells were incubated with 40, 80, and 160μg/ml sodium fluoride for 24 hours, the cell survival rate, the level of P53 protein and mRNA expression was assessed by MTT, Western blot and RT-PCR analysis respectively. The cell survival rate in L-02 cells treated by high concentration of fluoride was significantly lower than that in the control group.

方法］对体外培养的人胚肝细胞染毒氟化钠40、80、160μg/ml 24h后，分别采用MTT试验、蛋白印迹技术和逆转录－聚合酶链式反应方法检测人胚肝细胞存活率、P53蛋白和mRNA水平。

Methods Mesangial cells were incubated in the media with various concentrations of SPARC and its peptide cultured in vitro.

采用体外传代培养的HMC，用不同浓度的SPARC全长蛋白及其肽段分别作用不同时间。

The females incubated at 33 , their ovarium has powerful cortex, the germinal epithlium produce oocyte, its medulla degenerate into ovarian cavity. During sexual differentiation, mitochondria and smooth endoplasmic reticulum in cells of ovary increase in quantity, they disintegrate after sexual differentiation. There are a lot of big grana in cells of ovary before sexual differentiation, in the course of sexual differentiation the quantity of the big grana decrease, while small grana increase and peakafter sexual differentiation. The males incubation at 26 , cortices of their testes degenerate into a thin albuginea. Medulla are formed by testicular cords in which scatter spermatogonium.

33℃下孵化的小龟卵巢皮质发达，由原始生殖细胞产生卵原细胞，髓质退化成为卵巢腔，分化时卵巢细胞的超微结构显示线粒体和内质网的数量大大增加，它们在分化结束后解体，分化前细胞内有较多的大颗粒，在分化中大颗粒减少，小颗粒增多，在分化后小颗粒达到最多。26℃下孵化的小龟精巢皮质退化成为一层薄的白膜，髓质有许多睾丸管构成，管腔内有散落排列的精原细胞。

Changes on the shape of the statolith were observed among different incubated conditions. The shape of the statolith at hatching had obvious differences among different embryonic developmental conditions. The statolith developed in the colder environment had smaller dorsal dome, thinner and shorter rostrum than that developed in the warmer condition.

不同条件下培养的幼体在其平衡石的型态上有所差异，其型态上的变化随著培养的条件而有显著的改变，在较冷的条件下培养的个体拥有较短小的钝端比例之平衡石，而在较高温条件下培养的个体则是拥有较长比例钝端的平衡石。

METHODS: A series of N-octyl-N'-succinyl chitosan labeled with fluorescein, were incubated with HepG2 liver cancer cells, K562 leukemia cells, A549 human lung cancer cells, and BGC cancer cells respectively and followed by using flow cytometry and enzyme-linked immunosorbent assay to assess the affinity and inhibition of N-octyl-N'-succinyl chitosan for the four kinds of tumor cells already mentioned.

用异硫氰酸荧光素对N-正辛基－N'-琥珀酰基壳聚糖进行标记，再分别与人肝癌细胞(HepG2)、人白血病细胞(K562)、人非小细胞肺癌细胞(A549)及人胃癌细胞共培养，通过流式细胞仪及酶联免疫检测仪测定N-正辛基－N'-琥珀酰基壳聚糖对肿瘤细胞的亲和性及抑制力。

Based on data of teliospore germination of Tt2 on day 50 after incubated,the model of relationship between teliospore germination rates and temperature for Tt2 was constructed as Y =0.150 77exp-(0.029 49( X -4.957 6)2.These results of biological experiments will provide fundamental data for risk assessments of establishment of TCK in China.

根据分离菌Tt2不同温度下培养50 d时的萌发率，建立了萌发率与温度的模型为Y=0.150 8 exp[-0.02949( X -4.957 6)]2，此结果为TCK的风险评估提供了基础数据。

Methods Seven probe drugs were incubated with or without methyl protodioscin respectively in human microsomes. The specific substrates used in this study were caffeine (CYP1A2), dextromethorphan (CYP2D6), tolbutamide (CYP2C9),-mephenytoin (CYP2C19), chlorzoxazone (CYP2E1), coumarin (CYP2A6) and midazolam (CYP3A4). The concentrations of the substrate metabolites (paraxanthine, dextrorphan, 4-hydroxytolbutamide, 4-hydroxymephenytoin, 6-hydroxychlor-zoxazone, 7-hydroxycoumarin and 1-hydroxymidazolam) were determined by HPLC and LC-MS/MS. Results MPD 1-10 μmolL^(-1 had no significant inhibition on the activities of the seven cytochrome P450 enzymes.

将MPD和CYP450酶7种亚型的特异性探针底物咖啡因(CYP1A2)、右美沙芬(CYP2D6)、甲苯磺丁脲(CYP2C9)、s-美芬妥因(CYP2C19)、氯唑沙宗(CYP2E1)、香豆素(CYP2A6)及咪达唑仑(CYP3A4)与人肝微粒体进行孵化反应，采用HPLC和LC-MS/MS法测定对应的7种代谢产物（1，7-二甲基黄嘌呤、去甲右美沙芬、4-羟基甲苯磺丁脲、4-羟基美芬妥因、6-羟基氯唑沙宗、7-羟基香豆素和1-羟基咪达唑仑）的浓度，通过与对照组比较，确定MPD对以上7种酶活性的影响。

Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时，他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了