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Finally, PMCs were incubated in culture medium containing 10.0 mg/L of LPS for 0, 1, 3, 6, 12, and 24 hours, and the hexokinase activity and the glucose uptake of PMCs were detected.

最后用含10.0mg/L LPS的培养基孵育细胞不同时间（0、1、3、6、12、24h）；测定HK活性和细胞葡萄糖的摄入量，分析HK活性与葡萄糖摄入量的相关性。

Different concentrations of glucose (0.10%, 1.50%, 2.50%, and 4.25%) and LPS (0, 0.1, 1.0, 10.0, 50.0, and 100.0 mg/L) were added into culture media. Then PMCs were incubated in culture medium containing 10.0 mg/L of LPS and 4.25% of glucose for 0, 1, 3, 6, 12, and 24 hours. The hexokinase activity of PMCs was measured with standard glucose-6-phosphate dehydrogenase (G6PDH)-coupled enzymatic assay.

首先将含不同浓度的葡萄糖（0.10%、1.50%、2.50%、4.25%）分别和不同剂量的LPS（0、0.1、1.0、10.0、50.0、100.0mg/L）加入培养基；其次用含4.25%葡萄糖和10.0mg/L LPS的培养基孵育细胞不同时间（0、1、3、6、12、24h）；6-磷酸葡萄糖脱氢酶耦联比色法测定HK活性。

Secretion of sAPPα and expression of PKCα were found to increase after cells were incubated with HupA, suggesting a possible correlation between these two changes.

Western blotting结果显示sAPPα和PKCα蛋白水平均随着与石杉碱甲孵育的时间增加而增高。sAPPα的显著上升出现于PKCα上升之前，提示石杉碱甲对sAPPα分泌的调节可能与提高PKCα表达水平有关。

The mixture, incubated at rather less than body temperature, produced carbon dioxide and hydrogen but no pollutants.

这种混合，在低于体温的环境中发生反应，产生二氧化碳和氢气，但没有污染。

To inactivate the hydrolase, the hydrolysates were incubated at 70 or 100 C, and the effect for this two methods was similar.

牛初乳经胰蛋白酶和胃蛋白酶水解处理后，70℃灭活水解酶和100℃灭活水解酶对水解物影响小肠上皮细胞的效果无显著差异（P＞0.05）。

The cells were incubated in RPMI 1640 without or with different contents of glutamine and with 10%fetal calf sera, at 37℃,5% CO2 and saturation humidity for 4 days.The initial living cell density was 5×108/L. After 4 days incubation,the cells were counted,collected, smeard and stained with Wright-Giemsa, DNA, POX, NAE and NaF inhibition,gulping ink and NBT etc. cytochemical technology. The stained cells were investigated under oil immersion lens.

从18例未经治疗的APL病人外周血中提取APL原代细胞，在无Gln的RPMI 1640培养液中补加10%胎牛血清，以活细胞密度5×108/L接种细胞，在37 ℃， 5%CO2和饱和湿度下培养4 d，计数活细胞，并收集细胞行Wright-Giemsa、DNA、POX、NAE及NaF抑制试验、墨汁吞噬试验和NBT还原试验等细胞化学染色，于油镜下观察。

Setting: University hospital research laboratories Subjects and Interventions: NB4 and A549 cells were separately cultured with ATRA and/or dexsamethasone for 1-3 days. NB4 or ATRA -NB4 cells were then placed in an upper insert and co-incubated with A549 cells or their conditioned medium located in a lower plate.

材料与方法：将NB4 细胞与 A549 细胞分别经有／无全反式维甲酸及 dexamethasone 处理 1-3 天，再将有／无全反式维甲酸处理过之 NB4 细胞置入嵌入式培养之上层舱，下层舱中则置放有A549 细胞或各种不同处理的条件培养液，再将上层舱嵌入下层舱中，於含 5% CO2 之培养箱中共同培养。

Semi-quantitative analysis results showed that expression level of BmDHR was different in 5 instar larvae and pupa of bivoltine race incubated under different environment conditions.

定时半定量分析显示，BmDHR基因表达水平在不同条件催青的二化性5龄幼虫、蛹体中存在差异。

Methods In vitro, human gastric cancer cell line SGC-7901 in log phase was intervened by As_2O_3 (final concentrations in order:1.0,5.0,10.0μmol/L) and 5-Fu (final concentration:50mg/L) respectively alone or in combination, and incubated for 24h、 48h、 72h. Blank group is set up. The cell apoptosis and cell cycle changes were observed by flow cytometry, and the expression of apoptosis-related gene Bcl-2 was analyzed by immunocytochemical method and absolute gray scales quantitative method.

将对数生长期的人胃癌细胞株SGC-7901(终浓度为2×10~5)经低、中、高三种浓度的As_2O(终浓度分别为1.0，5.0，10.0μmol/L)和5-Fu(终浓度50mg/L)单独及联合处理，设空白对照组，分别孵育24h、48h、72h后，收取标本采用流式细胞术测定细胞凋亡率及细胞周期变化；免疫组化绝对灰度定量分析检测凋亡抑制基因Bcl-2的表达。

The number of His-EGFP-CRP detection peaks increased after the protein was incubated with lysate of THP-1 cells.

为解决这一技术难题，构建了带his纯化标签和EGFP荧光标记的人CRP原核表达质粒［4］。

Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时，他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了