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Methods HUASMC were cultured by the explant method and incubated with 50 mg/L, 100 mg/L and 200 mg/L WEP.

贴块法培养人脐动脉平滑肌细胞，用50、100和200 mg/L蜂胶水提液进行干预。

To evaluate the fermentability of oat β-glucan in vitro, mice faeces were incubated in oat β-glucan medium that contained 50mg OG〓 or OG〓 and fermented for 24h.

OG〓高剂量组在实验期间，造成小鼠体重下降，说明具有一定的抗营养作用；观察小鼠小肠组织形态，发现小鼠小肠绒毛变短，杯状细胞数目增加。

Objective To investigate the changes and effects of HSP7O/HSP9O of in nasopharyngeal carcinoma cells FINE1 after thermotherapy. Methods HNE1 cells were incubated at 42℃ for 2 h.

目的 探讨体外鼻咽癌细胞系HNE1细胞热疗后热休克蛋白70/90(HSP70/HSP90)的表达变化及抑制HSP70/HSP90表达对鼻咽癌热疗的影响，并初步探讨其机制。

Methods BSA incubated withglucose were subjected to PAGE and SDS-PAGE,fluorescence spectrum and fluorescence intensity were measured using fluorometer.

牛血清白蛋白与葡萄糖在体外共同孵育后，经聚丙烯酰胺凝胶电泳及荧光分光光度计测定荧光值和扫描荧光光谱。

This technique was modified as follows: the small rubber particles were purified and diluted to give a protein concentration of an absorbance at 600 nm of about 2.0～2.5; 0.5% basic fuchsin was prepared by being dissolved in 50 mmol/L Tris-HCl(pH7.4); the reaction mixtures were incubated for 30 min at 25 ℃, and centrifuged for 5 min at a speed of 5 000 r/min; and B-serum can serve as the protein samples for the positive control of rubber particle aggregation.

几个关键参数为：（1）使用纯化的小橡胶粒子，适宜浓度为在600 nm处的OD值为2.0～2.5；（2）用50 mmol/L Tris-HCl（pH7.4）配制0.5％碱性品红；（3）25 ℃条件下孵育30 min；（4）5 000 r/min离心5 min；（5）B乳清可作为阳性对照的蛋白质样品。

Furfur was inoculated to the mycelial culture liquid medium and the rapeseed oil culture liquid medium, and then incubated in vibrator to induce the production of mycelial phase and yeast phase of M.

用全脂牛奶平板法、Folin-酚法、QuantiCleave蛋白酶试剂盒测定菌丝相及酵母相糠秕马拉色菌胞外蛋白酶的活力，并检测温度、pH值对蛋白酶活性的影响。

Neutrophils from normal animals were isolated from whole blood and incubated with plasma from rat gracilis muscle flaps on coverslips pretreated with ICAM.

嗜中性粒细胞从正常动物的全血中分离且用ICAM预处理过的盖玻片覆盖来自于大鼠股薄肌皮瓣的血浆孵化。

However, the eggs of Tibetan chicken can maintain higher hatchability when incubated at high altitude.

以上结果说明藏鸡在高原能够维持较高的孵化率虽然与母鸡饲养环境有关，但主要是遗传的影响。

Once the eggs have incubated, which is normally anywhere between seven and nine days, the eggs will hatch and the life of a head louse will begin.

一旦蛋孵化，这是通常之间的任何位置7和9天，将蛋孵化和生活，一头虱将开始。

Quiescent RASMCs were incubated respectively in 10% FBS DMEM as control group, 10% FBS DMEM supplemented with 0.1% dimethyl sulfide as vehicle group and 10% FBS DMEM supplemented with panaxynol(at concentration of 1,3,9×10-6 mol·L-1) as panaxynol groups. 1. Cell counting and double time. Cell viability was examined with trypan blue exclusion test and the cell number was counted at 0, 24, 48, 72h after the incubation using a hemocytometer.

实验中细胞分成如下几个处理组：①正常对照组：含10％FBS的培养液；②溶剂对照组：0.1％DMSO；③药物处理组：panaxynol 1、3、9×10-6 mol·L-1组。1、细胞计数及倍增时间测定：于接种并同步化后处理0、24、48、72h，用血细胞计数板计算活细胞数量，绘制生长曲线并计算细胞倍增时间。

Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时，他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了