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After cultured with tumor cytolysis antigen for 2 days, the cells were incubated with auto T cell separated by E-rosette formation. Methyl thiazolyl tetrazolium assay was employed to test inhibition rate of tumor specific cytotoxic T lymphocyte on HO9810 tumor cell line. Results Our results indicated that DC precursors in cord blood can be induced to differentiate and mature in the medium containing IL-4, GM-CSF, and TNF-α.

将获得的DC与用 E-花环分离脐血单个核细胞得到的T淋巴细胞共同培养3 d，然后分为两组，实验组2继续与不同比例的HO9810细胞共同培养3 d后获得细胞毒T淋巴细胞，对照组2除加入HO9810外，还加入了无关细胞，用四甲基偶氮唑蓝法检测其对HO9810细胞的抑制作用。

Filtrate of cultured dermatophyte and candia albican was incubated with human keratinocyte,the level of growth factor and the effect were comparatively analysed.

取浅部真菌和白念珠菌培养滤液与原代培养人类角质形成细胞孵育，比较表皮因子值及其对角质形成细胞生长的作用。

Extensor digitorium longus and soleus muscles which represent two types of muscle fiber were studiedd. Results In the early postburn period as well as in septic states,the lactate production of muscle cells was significantly elevated as compared to the normal controls even though the muscles were incubated in a fully oxygenated media.The levels of aerobic glyoclysis,as well as its changes in postburn period and in septic states,vary depending on the difference in the fiber composition of the muscles.

结果 在可靠的肌肉组织充分供氧离体孵育系统中，两种肌肉组织在烧伤后和脓毒症情况下乳酸产生量均较正常对照组有不同程度的增加；不同类型的肌肉组织有氧糖酵解的水平及其在烧伤后和脓毒症状态下有氧糖酵解过程的改变具有各自不同的特点。

Every explant was pricked with a dissector at the center of one subregion before inoculated on MS+BA 1mg/L+NAA 4mg/L+2,4D 05mg/L medium and incubated in darkness for 7 days,then transfered to MS+BA 1mg/L medium.85% of the explants regenerated somatic embryos directly around the prick after 40 days.

每一叶片在1个亚区中心部位用解剖针刺伤1点后接种于MS＋BA1mg／L＋NAA4mg／L＋2，4D05mg／L＋蔗糖20g／L的培养基上，暗培养7d后，转接到MS＋BA1mg／L＋蔗糖20g／L的培养基上继续暗培养，40d后，85％的叶片在刺伤口周围发生直接类型体细胞胚胎。

The two lobes of thyroid gland from each mouse were separated into two groups and incubated in Earle's medium.

在相同的培养条件下，大鼠甲状腺过氧化物酶活性，实验组比对照组增高了约三倍。

The oxidative burst modulators were added and cells incubated for an optimized period of time prior to addition of the elicitor, unless stated differently.

氧化爆裂调制的补充和细胞的培养优化之前很长一段时间，增加了 elicitor ，除非表示不同的看法。

Methods Eukaryotic expression plasmid pCMVCD was constructed , and identified by re2 striction endoenzyme digestion. CD gene was transfected into NSCs from new2born Wistar rats using Lipofec2tamine2000. Positive clones (named NSCs/ CD cells) were screened by G418 presence. 52Fluorocytosine (52FC) ad2ministration of different concentrations were incubated with NSCs/ CD cells. NSCs/ CD cells viability ratios were mea2 sured by MTT assay.

通过构建真核表达质粒pCMVCD ，限制性内切酶消化鉴定后，采用Lipofectamine 2000 脂质体介导法转染新生大鼠室管膜下区神经干细胞(Neural stemcells ， NSCs)，G418 筛选阳性克隆，加入不同浓度的52氟胞嘧啶(52Flourocytosine ， 52FC)，MTT比色法测定NSCs的生存率。

EPC were harvested and incubated with vehicle or insulin (0.1, 1, 10 nmol/L).

胰岛素对于心血管的生理功能具有重要的调节作用，但其对EPC影响的相关报道较少。

Methods S.mutans were incubated respectively in 0.5%, 1%, 2%, 4%, 8%,12%, 16% erythritol or xylitol culture medium under anaerobic conditions. The A and pH value of the mediums were measured at 0, 2, 4, 6, 8, 10, 12, 18, 24 hours, following the profile plots by SPSS.

分别用含0.5%、1%、2%、4%、8%、12%、16%赤藓糖醇和木糖醇的TPY培养基在厌氧条件下培养变异链球菌，分别于0、2、4、6、8、10、12、18、24 h测量液体培养基的光密度值和pH值，运用SPSS描绘其变化曲线图。

Methods Three-dimensional gels of type I collagen containing rabbit keratocytes were incubated in the presence of different concentrations of exotoxin A (0.1,1.0,10 mg·L-1),cultivated for 24 h at 37℃, the change of keratocytes in morphology was observed under the light microscope,and the amount of lactate dehydrogenase was measured by enzyme linked immunosorbent assay.

含兔角膜基质细胞（1×105·mL-1）三维胶原凝胶表面添加提纯的不同浓度绿脓杆菌外毒素A（0.1、1.0和10 mg·L-1）后，放入无血清MEM培养液中37℃培养24 h，采用光学显微镜观察培养的兔角膜基质细胞形态学变化，采用酶联免疫吸附法检测损伤的兔角膜基质细胞释放的乳酸脱氢酶含量。

Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时，他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了